FastQCFastQC Report
Sat 31 Dec 2022
SRR5847454.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847454.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179730
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTGTATATCTCGTATGC11010.612585544984143No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTGTATATCTCGTATGCCGTCT10380.5775329661158404No Hit
ACACTGGGGACTGTAGCTTTGTAGTAAGTTTTGAAGTCAGGAAATGTGAGTC2400.13353363378400934No Hit
GCCCTTGGGATCCCTAAGTCTTACTAAACACATTCAAGGAGAAATGGGGAAG2300.1279697323763423No Hit
ACGTTCGGGTCAGCATCGTTGCCCATGGTTGGCACTCAGTAAACGTTGGTTG2220.12351861125020865No Hit
GGCACGATTGTTCTTTCGGTTCTTCTTCACCGGCCCACCTCGTTTTTGCCTA2120.11795470984254158No Hit
TCTTACGGGAAACCACCTTTAGCTGAGAGTATTATCATATCAATTGTTTCAA2020.11239080843487453No Hit
AATACGGGGCAAACTATCTCTCTTTGCAGGTGATTTGATTCTCTACCTAGAA1990.11072163801257441No Hit
GTGATAGGGAATTTGGACACAGAAAAATAAATGTATAGGGAGATAACTGTAT1950.1084960774495076No Hit
CTCGATACGGGTGCCTAGGATGATTGGAGTGTGGGGGAAATAGTTGCTTTCA1930.10738329716797419No Hit
CACAATGGGAAAGTCAGAAAGAAGAAAAAGAAGGGAACCATTACAGCAAATG1880.10460134646414064No Hit
GGACACGGGATTGTGAATACTTAATATATAACATCAAAAACTACAACTCCTA1870.10404495632337395No Hit
GTAATGGGGAAGTATTCTTCACGGCAGCCAGAAGTTAATGGTGGTAGCAGCT1870.10404495632337395No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)