FastQCFastQC Report
Sat 31 Dec 2022
SRR5847453.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847453.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178638
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTGTATATCTCGTATGC10810.6051344058934829No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTGTATATCTCGTATGCCGTCT9010.5043719701295357No Hit
ACGTTCGGGTCAGCATCGTTGCCCATGGTTGGCACTCAGTAAACGTTGGTTG2530.14162720137932577No Hit
GCCCTTGGGATCCCTAAGTCTTACTAAACACATTCAAGGAGAAATGGGGAAG2480.13882824483032727No Hit
ACACTGGGGACTGTAGCTTTGTAGTAAGTTTTGAAGTCAGGAAATGTGAGTC2260.1265128360147337No Hit
GTAATGGGGAAGTATTCTTCACGGCAGCCAGAAGTTAATGGTGGTAGCAGCT2200.12315408815593548No Hit
AATACGGGGCAAACTATCTCTCTTTGCAGGTGATTTGATTCTCTACCTAGAA2070.11587680112853929No Hit
TCTTACGGGAAACCACCTTTAGCTGAGAGTATTATCATATCAATTGTTTCAA2050.11475721850893986No Hit
GGCACGATTGTTCTTTCGGTTCTTCTTCACCGGCCCACCTCGTTTTTGCCTA2040.11419742719914017No Hit
TCCGTGGGAAATATTAAATATTCAATGAATGATAGCTGCCTCTACTTCTCCT1990.11139847065014163No Hit
TCTACGGGGACATTAAAGAAACTTCTTGCTCTAATAGTTATGTGTATGATAC1930.10803972279134338No Hit
GCCCAAGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC1890.10580055755214457No Hit
AGCTACGGGCTGGGGGCCCAGCCTCCCAACCGGCCAAGTCACAGTCTCTCCT1850.10356139231294573No Hit
TCCAAGGGTAACCTGAAATTTACTGCTATTTCAAATTCTACTCAATTTAGAG1830.10244180969334632No Hit
CTCACCGGGAGGAATAGATAAGGTTCTTGTCAACTCTAAGAAAATATAATTA1820.1018820183835466No Hit
CTCGATACGGGTGCCTAGGATGATTGGAGTGTGGGGGAAATAGTTGCTTTCA1820.1018820183835466No Hit
TGCAGGAGGGCAAGTGGAGCCCCTGGCCAAGAAGCCAGCCAAACCAGCAGAG1810.10132222707374691No Hit
TACGCAGGTCAGACACGAAAACCAGAACTGCATCAGGATATGGTGGTACCAG1810.10132222707374691No Hit
GCTGGCGGGAGAAGAGAAGTTTTGGAACATTCTTGACAGAAAGGAAAGATTA1810.10132222707374691No Hit
CACTAAGGGAAATGTTGGATATGTTGAAGCAGACAGGAAGGCCGGAGATGGT1790.10020264445414749No Hit
CGTGCTGGGTGGTAAAGGGATCAATTCAACAAGAAGAGCTAACTATCCTAAA1790.10020264445414749No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)