FastQCFastQC Report
Sat 31 Dec 2022
SRR5847436.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847436.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84917
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT12821.5097094810226457No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC10351.2188372175182827No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC2530.29793798650446907No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC1760.2072612080031089No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT1700.20019548500300294No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA1370.16133400850242002No Hit
TTCAGCGGGAGTCACAAGAAGTGGTGGAATGTTACGAGAAGCTATTGATTAA1160.13660397800204907No Hit
TATGAGGGGATTTTCTTTGTATAAACACTTCTACCTTCAAATTGTGTCATTA1100.12953825500194308No Hit
AATGCCGGGGACAGAATCTCCGTGTGCTTTGGTGGCCAGCCAGTACGGATGG1000.11776205000176643No Hit
AAATGGGGGAGCCATTGCTGTAACAACCTTATTTTCCACTAAGTCAGAATAA980.1154068090017311No Hit
CTGCTTGGGTGACAGTGCTTTATGAGCCTTAAATTCGGCCTCAGCTTTCTCC950.1118739475016781No Hit
ACACAAGGGATAATTATTACATTTAAGCTCTTGGGACATTACAATTGAATGT930.10951870650164279No Hit
GATACACGGGAGTGATTGATGACCTCGATATGGATTTAGATTATCAAATGTG920.1083410860016251No Hit
ATTTGCGGGAGTAAATTAACTACTTGTTTATTTGTGGTAATTTATTTTTAAT900.10598584500158979No Hit
TTGACTGGGGACCTCTATGTCAGTTACATCCATGTGCTGACAGTGGGCACAC890.10480822450157211No Hit
AATTTAAGGGCTTCTACAAATGCTGTGACAATGACAATTCTGTGAACACCTA890.10480822450157211No Hit
AGATTTGGGGAGGGGGCTGGGAATAGCCGCTCATGTCGGCTAACGGAGCGGT860.10127536300151914No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)