FastQCFastQC Report
Sat 31 Dec 2022
SRR5847435.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847435.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83865
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT12381.476181959100936No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC10941.3044774339712633No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC2580.30763727419066356No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT1840.21940022655458175No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC1770.21105347880522266No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA1400.16693495498718178No Hit
TATGAGGGGATTTTCTTTGTATAAACACTTCTACCTTCAAATTGTGTCATTA1170.13950992666785905No Hit
TTCAGCGGGAGTCACAAGAAGTGGTGGAATGTTACGAGAAGCTATTGATTAA1090.12997078638287723No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTATACAC1000.11923925356227269No Hit
GATACACGGGAGTGATTGATGACCTCGATATGGATTTAGATTATCAAATGTG980.11685446849102725No Hit
TTTGTGGGGACTCCATACATATTCTCTTATTAACTAAGACAGGTATGTGAGA960.1144696834197818No Hit
ATTTGCGGGAGTAAATTAACTACTTGTTTATTTGTGGTAATTTATTTTTAAT930.1108925058129136No Hit
GTGTGCGGGTAATAGACCCTATGTCATAATGGCACTTTAGTGTATTGTTCTG900.10731532820604543No Hit
GTTCACGGGGTGCCTTTGGGACATTCAAGAGTGGATATCAAGTAGACTGTTG890.1061229356704227No Hit
AAATGGGGGAGCCATTGCTGTAACAACCTTATTTTCCACTAAGTCAGAATAA880.10493054313479998No Hit
CAATCTGGGAAACTTTAAGCAGTGCATATTTATGTTATAGATAAGATAAACA840.10016097299230906No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)