FastQCFastQC Report
Sat 31 Dec 2022
SRR5847433.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847433.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67934
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC15632.300762504784055No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT15152.2301056908175583No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC2950.43424500250242887No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC2720.40038861247681573No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT2250.3312038154679542No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA1930.2840992728236229No Hit
ATAGTAAAGGGACAGGTGGATAACAAAACCTCATAGCCAATGAACAGGACAG1620.238466747136927No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCC1300.19136220449259575No Hit
TACGCAGGGATCTCCTAAGAATGTACCGAAGCCAGCAAATAAAGGATGCAAT1290.1898901875349604No Hit
AAAAACCGGGCTAGGGTTGTGTAATGCTTAGAAAGGACAATGTAGTCATATT980.1442576618482645No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTATACAC940.13836959401772309No Hit
CTAGTTGGGGAGAGAAACTTAGCTCTTTCATAAGCATGGGAAGCTTTGTTTT920.13542556010245238No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAT890.13100950922954632No Hit
TCGCCAGGGGGCATTGCTGCTAAGTACATGCTGGATTGTAGAACTTCTCTCT890.13100950922954632No Hit
TAAACAGGGGCAGTCTTTGACTTCTGAAATATACAGAAGACTTACTGTTTCT870.12806547531427562No Hit
CTGAAAGGGACCTTGAGAGAAGAAAGAAATTGACTTAGGAATAAAGAATTCT870.12806547531427562No Hit
CACATGGGGAGCCAGTGGTTGTGGACAACCATTTACTTGGTGTAAAGAACTT860.12659345835664026No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAACTCGTATGC860.12659345835664026No Hit
CTCGCTGAGGGAGCCTGGGGTTTTCATCTTCAATGTGCTGGATGGCGGTGTG860.12659345835664026No Hit
CATAATGGGGTTCTTGGAATCCAGATTTACTTCTGTTTTTTTCTTCCCTACG850.1251214413990049No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTA840.12364942444136957No Hit
GAAACAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGGCCGGGGTC830.12217740748373422No Hit
TAAAATGGGGAAGAGAGAGACTGAAGGTCCCTAGGAAGAATTCTGCCAAGGC810.1192333735684635No Hit
CACAATGGGATCCAATGACATGTGCACTAAAGCACTTCTGTGAAAAAATACA800.11776135661082816No Hit
AGTCCAGGGAACCTCCACCTCCTGGGTTCAAGCAGTTCTCCTGCCTCAGCTT790.11628933965319281No Hit
TTTGTAGGGGGAGAGACTTCCCCCAGGCAATTATAGGGTCTCCAAATAACAG780.11481732269555744No Hit
GGTGCTGGGGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATAGAGA780.11481732269555744No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGT770.11334530573792209No Hit
GCCAAAGGGCTTGTCTTTGTCTGCGGTTTGTCGTGACCTCTTTACTGCTTCA770.11334530573792209No Hit
TATCTTGGGAGATAAAATCTTCTTGCATGACAGAGGGAATTCGAACAGTTTC770.11334530573792209No Hit
TTTATGGGAAATCTAATAAGAACTAATATTTATGGAGTACTGATATTGCATT760.11187328878028675No Hit
TAGTAAGGGATTGATTGTCCTTTAAAAAAGAAAAGTGCATATTTATCCAAAA760.11187328878028675No Hit
TAAGCTGGGGATCTGACAGAGGGGCTATAGATTTAAATTCATTGATTAGTTT750.1104012718226514No Hit
CCATTTGGGATCCTTAAATTTATGTGTGCCACTTCACCTACCTCTTTCAATT750.1104012718226514No Hit
TAACAGGGGCTCAGCCATCCCGGGGGCTCCTCGCTAGCCAAGAGCCGGTTCC740.10892925486501603No Hit
AATCTCGGGCTCCAGCCATAGCTGGGCTTGGCATATTTGCACCATCCCGGGT740.10892925486501603No Hit
AGCACGGGGGGAGAACGCTGGTGGGCCTGTTGTGGAGTACGCTTTGGACTGA740.10892925486501603No Hit
GTAGTGGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT740.10892925486501603No Hit
TTTGTCGGGGATTCTGGTAAATGTATATTTATGCAGAGAAAATTTTCTAGAA730.10745723790738068No Hit
AGAATAGGGATACATAAACTGATCCTAAAAAATTCTTCCTACATTTCTGCAA720.10598522094974534No Hit
CTGTTAGGGGTAAAAAGTGAAAACTGAACAAGGTTGACAGTCTGGTTAATAG720.10598522094974534No Hit
ATCAAAGGGGTTGTTGTGTTAGTTTAAAACGATAATAAAAAGAAAGAAATTA720.10598522094974534No Hit
TTAAGCGGGGTAATACCATACCGCATTTTGAAAATAATTCAATATTCTGTTT720.10598522094974534No Hit
AACTGTGGGAACTGTTTTAATACCGGATTTTGTATTAAAACAACTTAAATGA710.10451320399210999No Hit
CGGCCTGGGAAATATAGGGTCATGTGTCACTTAATGGTGGAGATACATTCTG710.10451320399210999No Hit
GGTTAGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG710.10451320399210999No Hit
GGTGGTGGGATCACAATGTGAACTCTTCAAAATTGTCTCTCCATAACAGTTT710.10451320399210999No Hit
ACATATGGGGATATTAATAATACCTATCTCTTGGGTTAGTTGTGAATATCAA700.10304118703447464No Hit
GCGTAAGGGCTGCTAGAGGGGATTAGTGAAAAGAAAAAGATGGCGATAAGAA700.10304118703447464No Hit
ACCACCAGCCTCCTAAAAATAATTTTATTCAGTAAAAATAATCCTTAATATT700.10304118703447464No Hit
GAATATGGGAGGAAAAAGTGTTCAATGTAAAAGGGAAATTATGTTTACAAAG700.10304118703447464No Hit
TCTGTCCAAGGGATCTGAGTGGCACTAAATTTGTTCACCTGTCATTCTTCTA700.10304118703447464No Hit
GCATATGGGAGTTTTCTTTTTGCTTTTTAAATTGAAGTACCAGGTGTTTATA700.10304118703447464No Hit
ATAGTAGGGATCCAGTGAAGATGTTTTTAAAATGTGATTAATTAACATTTTT690.1015691700768393No Hit
TTATTAGGGGCATATGGATATCCAGTTGTCCCAGCTCCATTTATTAACGAGA690.1015691700768393No Hit
GCCGTGGGGACAGAAACAGATGTCTGGTTTTCTAAACAGAGGAAAGATTTTG690.1015691700768393No Hit
AGCAAAGGGCATCATAATCATGGTTCTCCTCACCTTAAGGCCAAGCATACCA690.1015691700768393No Hit
CGGTTTGGGTGGCTTTAATTATGTGATTGTATAAGAATGTTCTCAATGATCT690.1015691700768393No Hit
ACAACTGGGATTGTGAAGCTATAATTTCCTGGGTTATATTTTAGATGGGTTG690.1015691700768393No Hit
GCCAATCAGTCCTCACTCCCTTTCCCTTTCCCACAGAAGCAGACACTCTGAA680.10009715311920393No Hit
GAGTATGGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAA680.10009715311920393No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)