FastQCFastQC Report
Sat 31 Dec 2022
SRR5847432.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847432.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66789
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC15752.3581727529982484No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT15602.335713964874455No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC2750.4117444489362021No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC2720.4072526913114435No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT2320.34736258964799593No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA1840.27549446765185887No Hit
ATAGTAAAGGGACAGGTGGATAACAAAACCTCATAGCCAATGAACAGGACAG1530.22907963886268698No Hit
TACGCAGGGATCTCCTAAGAATGTACCGAAGCCAGCAAATAAAGGATGCAAT1350.20212909311413557No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCC1110.16619503211606704No Hit
TCGCCAGGGGGCATTGCTGCTAAGTACATGCTGGATTGTAGAACTTCTCTCT1010.15122250670020512No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTA1010.15122250670020512No Hit
CTCGCTGAGGGAGCCTGGGGTTTTCATCTTCAATGTGCTGGATGGCGGTGTG930.13924448636751563No Hit
CTGAAAGGGACCTTGAGAGAAGAAAGAAATTGACTTAGGAATAAAGAATTCT880.13175822365958464No Hit
AATCTCGGGCTCCAGCCATAGCTGGGCTTGGCATATTTGCACCATCCCGGGT870.13026097111799848No Hit
CTAGTTGGGGAGAGAAACTTAGCTCTTTCATAAGCATGGGAAGCTTTGTTTT820.12277470841006752No Hit
TAGTAAGGGATTGATTGTCCTTTAAAAAAGAAAAGTGCATATTTATCCAAAA820.12277470841006752No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTATACAC800.11978020332689514No Hit
TATCTTGGGAGATAAAATCTTCTTGCATGACAGAGGGAATTCGAACAGTTTC800.11978020332689514No Hit
AAAAACCGGGCTAGGGTTGTGTAATGCTTAGAAAGGACAATGTAGTCATATT800.11978020332689514No Hit
CTACTCGGGGAAGGTTTGAGGTAAGATATGCTGGAAAGGCAAGATGTAGGCT770.11528844570213657No Hit
ATATCTGGGATCTCATATTCTGCTCACCTCCTACCCCTGACAACAATTACTG770.11528844570213657No Hit
ATGACTGGGATGTTATCTCTGCTTCAGATGCACACTTTTTCTAACTGAGACA770.11528844570213657No Hit
ACAGTTGGGAAGAGGGTTTCAGAAAGAACAAGAATGAGGATAATTTTCCATG770.11528844570213657No Hit
TCTGTCCAAGGGATCTGAGTGGCACTAAATTTGTTCACCTGTCATTCTTCTA770.11528844570213657No Hit
GGTTAGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG760.11379119316055038No Hit
CACAATGGGATCCAATGACATGTGCACTAAAGCACTTCTGTGAAAAAATACA760.11379119316055038No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAACTCGTATGC760.11379119316055038No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGT750.1122939406189642No Hit
GCAGTAAGGGATAGTATATGATTTTAACATCACAGTCCATCTTCAAAAAATA750.1122939406189642No Hit
ACAACTGGGATTGTGAAGCTATAATTTCCTGGGTTATATTTTAGATGGGTTG740.110796688077378No Hit
TAAAATGGGGAAGAGAGAGACTGAAGGTCCCTAGGAAGAATTCTGCCAAGGC730.10929943553579181No Hit
GCGTAAGGGCTGCTAGAGGGGATTAGTGAAAAGAAAAAGATGGCGATAAGAA730.10929943553579181No Hit
GCGTGGGGGATATCAAAGAATTTCTTAAGTAAAACGGTACAAGCTGAACCAC730.10929943553579181No Hit
CAATAAGGGGGTTCTCCTGCCTCGGCCTCCGGGGTGGCTGGGGTTGCGGGCG720.10780218299420563No Hit
CATAATGGGGTTCTTGGAATCCAGATTTACTTCTGTTTTTTTCTTCCCTACG720.10780218299420563No Hit
GAGTGGGGGGTTTATGTTCTTTGTTCAGATTTCCAGGATAAAGACTTCATAT710.10630493045261943No Hit
TAAACAGGGGCAGTCTTTGACTTCTGAAATATACAGAAGACTTACTGTTTCT710.10630493045261943No Hit
AAAAAGGGGGGCAGGCCAGCCTGCAGTGACAGTGAGACATGGCTTTGACTTG700.10480767791103325No Hit
TTTGTAGGGGGAGAGACTTCCCCCAGGCAATTATAGGGTCTCCAAATAACAG700.10480767791103325No Hit
GTAGTGGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT700.10480767791103325No Hit
GTTGTCGGGAATTCATAAAAACAGAATGTAGAATGGTGGTTACCAGGGGTTC690.10331042536944705No Hit
ACCACCAGCCTCCTAAAAATAATTTTATTCAGTAAAAATAATCCTTAATATT690.10331042536944705No Hit
TTTATGGGAAATCTAATAAGAACTAATATTTATGGAGTACTGATATTGCATT690.10331042536944705No Hit
CACTACGGGAAGCTTAAAAATGACGCTATCTGCTTGTATGTGAGAGAGAGAG690.10331042536944705No Hit
GGTGGTGGGATCACAATGTGAACTCTTCAAAATTGTCTCTCCATAACAGTTT690.10331042536944705No Hit
TTTGTCGGGGATTCTGGTAAATGTATATTTATGCAGAGAAAATTTTCTAGAA690.10331042536944705No Hit
AGAATAGGGATACATAAACTGATCCTAAAAAATTCTTCCTACATTTCTGCAA680.10181317282786087No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAT680.10181317282786087No Hit
TTGATGGGAAGAGCTCATAACCGTAATAAAAGCCCCCACCTCCTTTGGTTAT680.10181317282786087No Hit
AGCGCTGGGGTTTTTGTGTTCAAAATGCATTATTTTATGGTAATTTACATTT680.10181317282786087No Hit
GATGCCGGGGCCAGGCTAGAGTGCAGTGGCACGATCTCAGCTCACTGCAACC680.10181317282786087No Hit
GGTTCTGGGATGCTATTGAAATTTAACTTTGTATGATGCTTAAAAACCACTA680.10181317282786087No Hit
CTGTTAGGGGTAAAAAGTGAAAACTGAACAAGGTTGACAGTCTGGTTAATAG670.10031592028627467No Hit
CACCTTGGGCTATTTTGATTTGGCATGACTACTTAATTTTTTAAAAAAATTG670.10031592028627467No Hit
TAAGCTGGGGATCTGACAGAGGGGCTATAGATTTAAATTCATTGATTAGTTT670.10031592028627467No Hit
GGCTAAGGGAAGAGAGTCTTAATCTGTCAAATTGATCAGATTGGATTTATCT670.10031592028627467No Hit
GGTGCTGGGGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATAGAGA670.10031592028627467No Hit
GCTGAAGGGTGGGATTTGCCTATCTATGTCATCAGTGTTGAGCCCGGAGGAG670.10031592028627467No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)