FastQCFastQC Report
Sat 31 Dec 2022
SRR5847431.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847431.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67205
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT15512.307863998214419No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC14632.176921360017856No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC2680.3987798526895321No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC2590.38538799196488355No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT2340.34818837884086007No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA1760.26188527639312553No Hit
TACGCAGGGATCTCCTAAGAATGTACCGAAGCCAGCAAATAAAGGATGCAAT1480.2202217096942192No Hit
ATAGTAAAGGGACAGGTGGATAACAAAACCTCATAGCCAATGAACAGGACAG1260.1874860501450785No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCC1260.1874860501450785No Hit
TCGCCAGGGGGCATTGCTGCTAAGTACATGCTGGATTGTAGAACTTCTCTCT1150.17111822037050817No Hit
CACAATGGGATCCAATGACATGTGCACTAAAGCACTTCTGTGAAAAAATACA1090.1621903132207425No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAT1010.15028643702105499No Hit
CTGAAAGGGACCTTGAGAGAAGAAAGAAATTGACTTAGGAATAAAGAATTCT930.13838256082136746No Hit
GGTGGTGGGATCACAATGTGAACTCTTCAAAATTGTCTCTCCATAACAGTTT900.13391860724648463No Hit
AAAAACCGGGCTAGGGTTGTGTAATGCTTAGAAAGGACAATGTAGTCATATT870.1294546536716018No Hit
GTTGTCGGGAATTCATAAAAACAGAATGTAGAATGGTGGTTACCAGGGGTTC860.12796666914664087No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGT860.12796666914664087No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTATACAC850.12647868462167994No Hit
ATGACTGGGATGTTATCTCTGCTTCAGATGCACACTTTTTCTAACTGAGACA840.12499070009671899No Hit
GCCAAAGGGCTTGTCTTTGTCTGCGGTTTGTCGTGACCTCTTTACTGCTTCA840.12499070009671899No Hit
GGTGCTGGGGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATAGAGA840.12499070009671899No Hit
ATCAAAGGGGTTGTTGTGTTAGTTTAAAACGATAATAAAAAGAAAGAAATTA820.12201473104679712No Hit
GCCGTGGGGACAGAAACAGATGTCTGGTTTTCTAAACAGAGGAAAGATTTTG790.1175507774719143No Hit
TATCTTGGGAGATAAAATCTTCTTGCATGACAGAGGGAATTCGAACAGTTTC790.1175507774719143No Hit
TAAAATGGGGAAGAGAGAGACTGAAGGTCCCTAGGAAGAATTCTGCCAAGGC780.11606279294695335No Hit
CGGTTTGGGTGGCTTTAATTATGTGATTGTATAAGAATGTTCTCAATGATCT780.11606279294695335No Hit
GTCTCAGGGGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTAC770.1145748084219924No Hit
ACCACCAGCCTCCTAAAAATAATTTTATTCAGTAAAAATAATCCTTAATATT770.1145748084219924No Hit
TAAACAGGGGCAGTCTTTGACTTCTGAAATATACAGAAGACTTACTGTTTCT770.1145748084219924No Hit
ACAACTGGGATTGTGAAGCTATAATTTCCTGGGTTATATTTTAGATGGGTTG770.1145748084219924No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAACTCGTATGC760.11308682389703147No Hit
GCGTAAGGGCTGCTAGAGGGGATTAGTGAAAAGAAAAAGATGGCGATAAGAA750.11159883937207055No Hit
CTTACACGGGCAACCACAGCCAGAGAGCGAGTGCCCGCCACAAAGACGGTGC750.11159883937207055No Hit
CTCGCTGAGGGAGCCTGGGGTTTTCATCTTCAATGTGCTGGATGGCGGTGTG750.11159883937207055No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTA740.1101108548471096No Hit
CATAATGGGGTTCTTGGAATCCAGATTTACTTCTGTTTTTTTCTTCCCTACG720.1071348857971877No Hit
CTATAACCGGGGATGAGGAAATAAGACATACAGTCACAAATTCCCAAAAAAT720.1071348857971877No Hit
TAACAGGGGCTCAGCCATCCCGGGGGCTCCTCGCTAGCCAAGAGCCGGTTCC720.1071348857971877No Hit
GTAGGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTGAG710.10564690127222677No Hit
TTTATGGGAAATCTAATAAGAACTAATATTTATGGAGTACTGATATTGCATT700.10415891674726582No Hit
GTTTATGGGAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAA700.10415891674726582No Hit
TACGCTAGGGTGGCACCTAGCACTGGGTTTCTCTCAGCAGACCTCTATTAAA700.10415891674726582No Hit
ATGAAGGGACCAGAGTGCTTTGTATGTATGTCCCGTTTATACACATACATTA690.10267093222230488No Hit
ACAGTTGGGAAGAGGGTTTCAGAAAGAACAAGAATGAGGATAATTTTCCATG690.10267093222230488No Hit
TAGAGCCGGGTCATTAAATGACCGTGCTACAGGTTTCAAAGGATTTTAAGAT690.10267093222230488No Hit
AAAAAGGGGGTCAGACTAGCTATATTTTAGAGAGTTTGTTAAATGCAGTAAG690.10267093222230488No Hit
GTAGCCGGGAAGAGTGGCTCACTGTGCCTAAACTGTTCGTACAAACAATGTG680.10118294769734394No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)