FastQCFastQC Report
Sat 31 Dec 2022
SRR5847427.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847427.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91356
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT21702.3753229125618462No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC18822.0600726826918865No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC4450.48710538990323565No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC4280.46849686939007834No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT2880.3152502298699593No Hit
TAAACGGGGGTAGAATACAATGCATTGTTAGTCATGATTTCCATAAAGCTTC2450.2681816191602084No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA2180.23862691010989973No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGT1850.20250448793730025No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAT1510.1652874469109856No Hit
AATGATGGGGAAGGAGTGAAGTTCGATGAAAGTGAGAAAACTAAGGAGAGTC1470.16090897149612504No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTATACAC1370.1499627829589737No Hit
CGGTAAGGGATGGCAAAAATACTTCCCTGAGAATAAAGGCACCTAAGCTCTG1350.14777354525154343No Hit
CAAACCGGGCTGCACATCCTAGCAGCAGTAGCACCATATGTGACTTTGGGGT1320.144489688690398No Hit
GTTCCAGGGTGACACTGTTCCCACACTGAGTTGTTTTGGCTGCAGGGGTGGG1290.14120583212925258No Hit
TATTTGAGGGGATCTTGAGTAGGGTAATCTTGTTATTATAATTGAAATGGTA1280.14011121327553747No Hit
AGTTTGGGAAGATAAAGTCTCTGGATCTAATACGTAAACAAAGTTGTGATGC1280.14011121327553747No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGT1270.13901659442182232No Hit
ATAAATGGGGTATCCTTAAGGTTATAACTGAAAGAAATTGGATTTAAAGATT1260.13792197556810717No Hit
CACAGAGGGGGGAGTGGCTGTGGCAATTTCTTAAAATAAGACAACAATGAAG1200.13135426244581636No Hit
CATCGGGGGAAGGAATAGCAGCTTATGAAATCTTTTTTTAATAGTCAGATGA1190.1302596435921012No Hit
CTATGGGAAAATTGTTATGTGTGATTTGATCCTGTCATCATGATGCCAGCTG1120.12259731161609529No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTA1110.12150269276238014No Hit
CACTAGGGCTGAAGGATCATCTATGTATTCAAGTAATTATTTTTTAGATAAT1060.11602959849380445No Hit
GGGTATGGGTAACTTTTGTCAAAGAAATACTCTCAGAAGAGAAATGGGGCTT1020.11165112307894391No Hit
TCAGCTGGGGCAGTGAGGGAAGAGATAACCAAAATATTGCATCCTGATTAGT1020.11165112307894391No Hit
AACAGTGGGAATCTCATAAATAGAAAGAAAAATAATCTAGAAATTTTTCAAA1000.10946188537151365No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTC980.10727264766408337No Hit
TCACCCGGGGATCTGGTATAGGGATCAGATTTCTGTAAATAGCATTCTAAAG980.10727264766408337No Hit
TCTCTTGGGCTTCCGATCTGCGGTAGGGGGACTGAAAGAAGTAAATTATAAT980.10727264766408337No Hit
GCATGTGGGGTATTGAAAAAAGTAAGGGATTTAATGTAATCCAATATTAGCT940.10289417224922282No Hit
GCTGCCGGGTACCACATCTACTGACATTTGGCAGTGTCTTCCATATAGTAAG940.10289417224922282No Hit
TATCGAGGGTCATATATGTGGTCTGTTTTTGACTGAAATGATGTCATGTGGT930.10179955339550768No Hit
ATGCTGGGGATCTCAGTATGGGATTGGAAAGGAAGAAGTAAAACTTTCTGTT930.10179955339550768No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTC930.10179955339550768No Hit
AAGAATGGGGAACCACAGTGTTTTATTTATTTTTCTAAAACTCTTACTAAAT920.10070493454179255No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)