FastQCFastQC Report
Sat 31 Dec 2022
SRR5847425.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847425.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89456
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT22152.476077624754069No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC17942.005455195850474No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC4520.5052763369701306No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC3770.42143623680915754No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT2930.3275353246288678No Hit
TAAACGGGGGTAGAATACAATGCATTGTTAGTCATGATTTCCATAAAGCTTC2150.2403416204614559No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA2080.23251654444643174No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGT1820.2034519763906278No Hit
AGTTTGGGAAGATAAAGTCTCTGGATCTAATACGTAAACAAAGTTGTGATGC1350.1509121802897514No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAT1320.14755857628331248No Hit
CTATGGGAAAATTGTTATGTGTGATTTGATCCTGTCATCATGATGCCAGCTG1280.14308710427472723No Hit
ATAAATGGGGTATCCTTAAGGTTATAACTGAAAGAAATTGGATTTAAAGATT1250.13973350026828832No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTATACAC1250.13973350026828832No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGT1180.13190842425326416No Hit
CAAACCGGGCTGCACATCCTAGCAGCAGTAGCACCATATGTGACTTTGGGGT1170.13079055625111788No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTA1170.13079055625111788No Hit
CGGTAAGGGATGGCAAAAATACTTCCCTGAGAATAAAGGCACCTAAGCTCTG1160.12967268824897157No Hit
TATTTGAGGGGATCTTGAGTAGGGTAATCTTGTTATTATAATTGAAATGGTA1160.12967268824897157No Hit
AATGATGGGGAAGGAGTGAAGTTCGATGAAAGTGAGAAAACTAAGGAGAGTC1140.12743695224467894No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCC1110.12408334823824003No Hit
GTTCCAGGGTGACACTGTTCCCACACTGAGTTGTTTTGGCTGCAGGGGTGGG1080.12072974423180112No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTC1060.1184940082275085No Hit
CAGCGGGGGAGACACAAGCTCATAGGTGGAATGAACGTGACTTGGGTATCAG1030.11514040422106958No Hit
AAAACTGGGTCAAGATCCTTTCTCTCTGACTGAAAGGTCTCTCACCACTAGA1010.11290466821677696No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAACTCGTATGC1010.11290466821677696No Hit
CACTAGGGCTGAAGGATCATCTATGTATTCAAGTAATTATTTTTTAGATAAT980.10955106421033806No Hit
TCAGCTGGGGCAGTGAGGGAAGAGATAACCAAAATATTGCATCCTGATTAGT970.10843319620819174No Hit
TATGTTGGGAAGTAGGGCACTAATACCTTTTCTTATTCAAACTGAGTTACAG930.10396172419960652No Hit
TCTAAACGGGGGGTGTCCCCAGACCTCTAACCCTTGCCCCTAGCAGCCTCTT930.10396172419960652No Hit
GCTGCCGGGTACCACATCTACTGACATTTGGCAGTGTCTTCCATATAGTAAG920.10284385619746021No Hit
CATCGGGGGAAGGAATAGCAGCTTATGAAATCTTTTTTTAATAGTCAGATGA920.10284385619746021No Hit
GGGTATGGGTAACTTTTGTCAAAGAAATACTCTCAGAAGAGAAATGGGGCTT920.10284385619746021No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)