FastQCFastQC Report
Sat 31 Dec 2022
SRR5847424.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847424.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52498
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT25824.918282601241952No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC24114.5925559068916915No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC4260.8114594841708256No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC4020.7657434568935959No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT2940.5600213341460627No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA2940.5600213341460627No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTATACAC1490.2838203360128005No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGT1320.25143815002476283No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAT1280.24381881214522458No Hit
TACACTGGGAGGAACTAAAATGTCTATAAAGGCTTCTTAGGGGAGCAAGAGA1270.24191397767534No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCC1190.22667530191626348No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTA1150.21905596403672523No Hit
AGATCTGGGGGGCTGTGGAGAGAGCGCGGAGACAAGCGCAGAGCGCAGCGCA1130.2152462950969561No Hit
TTTCGTGGGGGGGAGCCGGGGTAGTCGCCGCCGCCGCCGCCGCTGCAGCCAC1100.20953179168730238No Hit
GCATGTGGGATGACTGAATGTGAGCTAAGCATTTTCTCCTGTGGAGCCCTAG1060.20191245380776413No Hit
AGTTATGGGATTGCGGCAGTGGTACCTTGCCTTCAAGACTTGGGAAATCTGA1030.19619795039811042No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAACTCGTATGC1030.19619795039811042No Hit
TTATGCGGGGTCACACTTCCCGCCCCACACTCAACTGTTGTGTGAATGAGCC1020.19429311592822585No Hit
TACCTGGGGACTTTTAAAGATGAGTAAAATTCAGATCAGCAAAGATGGGGGT1020.19429311592822585No Hit
TAAAAAGGGTGGAAGAGGAGTCTCTGGGCTCGGTGCCAGCGCCTGCCTGCGC980.18667377804868757No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACCTCGTATGC970.184768943578803No Hit
GATGCAGGGGGGATAACCTCTGTGCGCCCCAGGGTTCAATGTTTGGGTGGAG960.18286410910891843No Hit
GTAATAGGGGAATTTTTGTCATGTAAAAATATTAAATTGCAAAATCAAAATC940.17905444016914931No Hit
CAAAAAGGGGCTGTAAGAATTTCTTAAAATCTGGCATATTTGTCCTTGTCAA900.17143510228961104No Hit
ACCTAAGGGGAATGTATTTTCTTCTGTGCTTTTCACTTCTCAGTAGTTTGTT880.1676254333498419No Hit
GCATGAGGGGTTGTACCAGTTTCTTAATGATATTTTGTTACTTCTAGAATAT880.1676254333498419No Hit
AATACTGGGAACATTTTAATTCTGTAAAAAGAATAGAACATGCTCACTGATC860.16381576441007278No Hit
GCGAATGGGGACACTGGAGATTGCTTGAAGAGGAAGGGTGGTAGGGAGCTAT850.16191092994018821No Hit
CAACTAGGGCTGTTCAAAGGAAATTTTAATTTCAGTCTACCAGAAAAGTTAA850.16191092994018821No Hit
CCACTTGGGGTGTCAAAGAAGAAATTGAAAAGGAAACTAGAAAGTGTATAAG830.15810126100041907No Hit
TTTGCTGGGGTGGTCATGGTCACCCAGGAGAGTGACAGCAGCTTTCTGGTCA830.15810126100041907No Hit
CGACTGGGGGTCAAGACACCCTCTTCCTGAGGGCCAAGGATGAGGCTAGTGC810.1542915920606499No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACCTCGTATGCCGTCT800.15238675759076536No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTC790.1504819231208808No Hit
ACAAAAGGGATGGCGCCACTGCACTCCAGCCTGGCGACAGAGCAAGACTCAG790.1504819231208808No Hit
ACAGTTGGGAAAACTCTGTCATATAAGTGACTACCATGGATGAAATAGGTGT780.14857708865099623No Hit
AGATTTGGGAAGGGACATCTGTATTTAACTCCTTTGTAGACATGAATTTCTA770.14667225418111166No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTC770.14667225418111166No Hit
ACTCATGGGAACTTCACTATAGTAGAAAATATCAACTACCATCCTTTTCTCT760.14476741971122709No Hit
TTTAGCGGGCTCTGGGCCCAGAGGGAGAAGAGCTGGCGAGAGTGGCAGAGGG760.14476741971122709No Hit
AACGCAGGGAGGTATGAGCTGTTTTAGAAGCAGCGTGTTGCCTTCATCTCTC750.14286258524134252No Hit
TTAGCCAGGGGGAGATCATCGAGGCTATCTACAAAATGGTAGGCACTGTGAT740.14095775077145795No Hit
CGAAGTGGGATATCACTATATATTGATTTTTATTTTATATATTTCCATGTAA730.13905291630157338No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCTGTCTCTTATA730.13905291630157338No Hit
CATTAGGGGCAAGCGAATAACTGTGACTATTTAGACAGCATTTTATTTTGTA720.13714808183168883No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCA720.13714808183168883No Hit
TTTTGGGGAACAGAATCATATCCTTAAGGATGACCATCATTCCCCCAACTAA720.13714808183168883No Hit
GCGCGCGGGAAACTTATAGATAAATGATGACTAATGGGAACGTAATGCATGT710.13524324736180426No Hit
ATGCAGGGGATTTGATAAAGATGGCGATGGCACCATCACAACAAAGGAACTT710.13524324736180426No Hit
TAACATGGGCTTATTCCTTTGTATGATAGAAGAGTAGATTTTAAAATATAAG700.1333384128919197No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCTGTCTC700.1333384128919197No Hit
GTTAGCGGGACCTGGGGGTGTCCCAGGGACTCTGTCAGTGCCTTCAGCCCAC690.13143357842203512No Hit
AGGCATGGGATCTCTATTTTTCTTAGAGGTTTATTAATTTTATTGACCATTT690.13143357842203512No Hit
AGCATCGGGGTGGCATGGCCCTGTTTCCTGTTGATATGGTGATAGTTGGAGA680.12952874395215055No Hit
TACTTTGGGCTGAGCTACTAAGGAGACTGAGACGGGAGAATTGCTTGAACCC680.12952874395215055No Hit
TGCTTGGGGCATGTCGGGACAGATGCAGATGCCATGAAGTACACCATTGTGG680.12952874395215055No Hit
CAGACTGGGAAGATTGGAGGGTAGGTGATGGTAGAAAATTCTGCGGCAAACA680.12952874395215055No Hit
AATTTGGGGATTTCTCAATCTGTGAGTAATGGACTAATTGCTATTAAATTTG670.12762390948226598No Hit
CTTTAAGGGATAACAAAACATCTGGGCTTTATAAGTATGTAAAGAATAATGA660.12571907501238141No Hit
AGGCTTGGGGTTATAAGTGTGTAGGAGTTATAACTTATTTTAAGAAGCAGTA660.12571907501238141No Hit
CAGCCAGGGAAGGGAGCCCCTAGCCCCTGGCAGACATAGCTGCTTCAGTGCC640.12190940607261229No Hit
TTATCGGGGAGGGCCATGTCAGAGGACACCAGGGTGGCCAGGAGGAGAAGCC630.12000457160272772No Hit
GTCACGGGCCCAAGAGGGTCTGCTCTGAGCCTGCGTTCCTAGGTGGCTCGGC630.12000457160272772No Hit
ATACTAGGGACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGA630.12000457160272772No Hit
CTTATGGGGGGCAGTTCTCCTCCACTGCCTTATCCTTCAGCTGGCCAGCCCA610.1161949026629586No Hit
ATGAAAGGGGGAAGTTAATACAAAAAAATTTCAAATTATTTCTATTGTAAAT610.1161949026629586No Hit
TACACTGGGGTCTGGCTGAAGATTTGATGTGGTTCCTCCTTAAGCTATGCGT610.1161949026629586No Hit
CTGTCTGGGCATTCCCATACACAGAAACACAAAACACTTTGATCATAAACTT610.1161949026629586No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATG610.1161949026629586No Hit
TTCAAAGGGGGGGCATGAAGCACATTAATTTGCAGTGGCTGCTTCATATGAG610.1161949026629586No Hit
GACGTGGGAAGGATATCTACGGCAACACTGCTCTCCATTATGCCGTGTATAA610.1161949026629586No Hit
TTGTAGGGGATATGATGAGACGCGGAGTGGTAGTGCTCACGAATACAGCTCC610.1161949026629586No Hit
CTTGAAGGGGTGCATAGAAAAGCTATTTAATATTTATCTTGTATCCAGGTAT600.11429006819307402No Hit
GTGCCGGGGACAGCACTCTATTTAAGTAGTATGTTTCAGTCATACTCCTATT600.11429006819307402No Hit
CATGACGGGGTTTTTACTCAGTAGACAGGACGGAAAAGTCTAGAACTGTCAA600.11429006819307402No Hit
TCTGCCCGGGATCCTGTCGCCTTGCTGCTTGTCAGCTCACACCTGCTAGTTG600.11429006819307402No Hit
ATCCGGGGAAGTCTATGAACTGTTAAATCAGCATTCATTTTAAGAAAAGCAA600.11429006819307402No Hit
GTGTCTGGGGACTCTCATGTGACCAATCAATCCCTGTTGGAAGCTGGGCCCT600.11429006819307402No Hit
AAGCTGGGGTAAAGTTTGGGACTTATAGACTTCTTGTTCTGATGTCACGTCC600.11429006819307402No Hit
CACATAGGGAAACATTAAAAAGTACCGCATGTTGGAGAGGTTGTTTAGATAC590.11238523372318945No Hit
CCCAAAGAGCCATTTCAACAGAGAAGGGAAATGACAAAGGGGCAGCTGGCCA590.11238523372318945No Hit
CAATTGGGGAAAAACATATCATGTAATTCAAAAACACTATTGATATTGAATA580.1104803992533049No Hit
GTATGCGGGAACCTAAAAACATGGAGCCAAGCTAAAAATGCCCTGGGGGTAA570.10857556478342033No Hit
CTTAAAGGGAGAGGGAGATACAGAAACCGACAGGGGCCAGGCGCCCGGTGGC570.10857556478342033No Hit
GGACACGGGGGTCACCAGAAAGACCAATTATGTAATTAGAAGAACTTTCAGC560.10667073031353576No Hit
AAGCGCGGGGTTGTGCTGTGGGGAAGGGAGAAGGATTTGTAAACCCCGGAGC560.10667073031353576No Hit
AAACCAGGGTCACTATCCGTGATCCAGTCTTAATTTGAGCATGAGAGCAAAA560.10667073031353576No Hit
CGCTGAGGAAATTGACTTTGAATATCCTTTTAGACTCTTTTGTTTTAGAAAG560.10667073031353576No Hit
GTCAAGGGGACTCACACATTCTTGGGGAAAAAAATCAAATGTCAGTCCTAGC560.10667073031353576No Hit
CTCTTTTAGGGAGATGATAATATGCTAAATTTTCCTCTATCCTGTACATTTC560.10667073031353576No Hit
CCGCCCGGGATCGATCAATTTGGGGAACACTGACATCTTTGTTATATTGAGT550.10476589584365119No Hit
ACTTCGGGGTGCTGGCTTTAGCAGAGAATAGGAGGAACCATATGAAAAAGAT550.10476589584365119No Hit
CAAAATGGGATATTAGTCAAACCAGAATTCAGATTCTAAATTTATCTAATGG540.10286106137376663No Hit
AACGTAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC540.10286106137376663No Hit
TTTAATGGGCAATCTGATTTTCCCATTCTTACTACTTCACAGCCATGAATGT540.10286106137376663No Hit
CGTATTGGGATGTGAATATGGCTCTTGTAAAGGATTAACTATTGTAATTTTA540.10286106137376663No Hit
CTTTATGGGGGGATGAGAGTTTTGGATGCAGTGCTCGCTGTTGCTGAATAGG540.10286106137376663No Hit
TAGTGAAGGGATTGTTAGAAATAATTACATTAGTCTTGTACCATGAACATTA540.10286106137376663No Hit
GGCCTCGGGGAGCACTGAAATATGAAAGGTATGTGAGAAAAGTGCCATCCAC530.10095622690388206No Hit
AGTTATGGGATGAAGGAGATGAAATAAGCATGATGCTGGTGCTGTCCAGACA530.10095622690388206No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)