FastQCFastQC Report
Sat 31 Dec 2022
SRR5847423.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847423.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52362
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT24924.759176502043467No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC24254.631221114548718No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC4650.88804858485161No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC3810.7276269050074481No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT2920.557656315648753No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA2820.538558496619686No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTATACAC1860.355219433940644No Hit
TACACTGGGAGGAACTAAAATGTCTATAAAGGCTTCTTAGGGGAGCAAGAGA1430.2730988121156564No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGT1390.2654596845040296No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAT1380.26354990260112293No Hit
TACCTGGGGACTTTTAAAGATGAGTAAAATTCAGATCAGCAAAGATGGGGGT1330.2540009930865895No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAACTCGTATGC1180.22535426454298918No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTA1180.22535426454298918No Hit
TTTCGTGGGGGGGAGCCGGGGTAGTCGCCGCCGCCGCCGCCGCTGCAGCCAC1170.2234444826400825No Hit
TAAAAAGGGTGGAAGAGGAGTCTCTGGGCTCGGTGCCAGCGCCTGCCTGCGC1140.21771513693136244No Hit
CAAAAAGGGGCTGTAAGAATTTCTTAAAATCTGGCATATTTGTCCTTGTCAA1100.21007600931973572No Hit
TTATGCGGGGTCACACTTCCCGCCCCACACTCAACTGTTGTGTGAATGAGCC1080.2062564455139223No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCC1080.2062564455139223No Hit
GATGCAGGGGGGATAACCTCTGTGCGCCCCAGGGTTCAATGTTTGGGTGGAG1040.19861731790229556No Hit
CGAAGTGGGATATCACTATATATTGATTTTTATTTTATATATTTCCATGTAA1040.19861731790229556No Hit
TTTAGCGGGCTCTGGGCCCAGAGGGAGAAGAGCTGGCGAGAGTGGCAGAGGG1040.19861731790229556No Hit
GCATGAGGGGTTGTACCAGTTTCTTAATGATATTTTGTTACTTCTAGAATAT960.18333906267904207No Hit
CGACTGGGGGTCAAGACACCCTCTTCCTGAGGGCCAAGGATGAGGCTAGTGC950.18142928077613535No Hit
GCATGTGGGATGACTGAATGTGAGCTAAGCATTTTCTCCTGTGGAGCCCTAG950.18142928077613535No Hit
TTTGCTGGGGTGGTCATGGTCACCCAGGAGAGTGACAGCAGCTTTCTGGTCA920.1756999350674153No Hit
GCGAATGGGGACACTGGAGATTGCTTGAAGAGGAAGGGTGGTAGGGAGCTAT910.1737901531645086No Hit
GCGCGCGGGAAACTTATAGATAAATGATGACTAATGGGAACGTAATGCATGT910.1737901531645086No Hit
ATACTAGGGACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGA890.16997058935869525No Hit
AGATCTGGGGGGCTGTGGAGAGAGCGCGGAGACAAGCGCAGAGCGCAGCGCA890.16997058935869525No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCA870.16615102555288186No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTC860.16424124364997517No Hit
AGTTATGGGATTGCGGCAGTGGTACCTTGCCTTCAAGACTTGGGAAATCTGA860.16424124364997517No Hit
CCACTTGGGGTGTCAAAGAAGAAATTGAAAAGGAAACTAGAAAGTGTATAAG860.16424124364997517No Hit
AACGCAGGGAGGTATGAGCTGTTTTAGAAGCAGCGTGTTGCCTTCATCTCTC860.16424124364997517No Hit
GTTAGCGGGACCTGGGGGTGTCCCAGGGACTCTGTCAGTGCCTTCAGCCCAC830.1585118979412551No Hit
AGGCATGGGATCTCTATTTTTCTTAGAGGTTTATTAATTTTATTGACCATTT820.15660211603834842No Hit
ACAGTTGGGAAAACTCTGTCATATAAGTGACTACCATGGATGAAATAGGTGT800.15278255223253506No Hit
AATACTGGGAACATTTTAATTCTGTAAAAAGAATAGAACATGCTCACTGATC770.14705320652381498No Hit
ATGAAAGGGGGAAGTTAATACAAAAAAATTTCAAATTATTTCTATTGTAAAT770.14705320652381498No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATG770.14705320652381498No Hit
GTAATAGGGGAATTTTTGTCATGTAAAAATATTAAATTGCAAAATCAAAATC770.14705320652381498No Hit
TCTTGTGGGGTAACCTAAAAAGTTTTGGCATATTTAGGTCAGCCTAGCGGAA770.14705320652381498No Hit
ACTCATGGGAACTTCACTATAGTAGAAAATATCAACTACCATCCTTTTCTCT740.1413238608150949No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCTGTCTC730.13941407891218824No Hit
GTGTCTGGGGACTCTCATGTGACCAATCAATCCCTGTTGGAAGCTGGGCCCT730.13941407891218824No Hit
ACAAAAGGGATGGCGCCACTGCACTCCAGCCTGGCGACAGAGCAAGACTCAG730.13941407891218824No Hit
AGCATCGGGGTGGCATGGCCCTGTTTCCTGTTGATATGGTGATAGTTGGAGA710.13559451510637485No Hit
ATGCAGGGGATTTGATAAAGATGGCGATGGCACCATCACAACAAAGGAACTT710.13559451510637485No Hit
CAGACTGGGAAGATTGGAGGGTAGGTGATGGTAGAAAATTCTGCGGCAAACA710.13559451510637485No Hit
TTAGCCAGGGGGAGATCATCGAGGCTATCTACAAAATGGTAGGCACTGTGAT710.13559451510637485No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCTGTCTCTTATA700.13368473320346816No Hit
CATTAGGGGCAAGCGAATAACTGTGACTATTTAGACAGCATTTTATTTTGTA690.13177495130056147No Hit
TAACATGGGCTTATTCCTTTGTATGATAGAAGAGTAGATTTTAAAATATAAG690.13177495130056147No Hit
AGGCTTGGGGTTATAAGTGTGTAGGAGTTATAACTTATTTTAAGAAGCAGTA690.13177495130056147No Hit
AAGCTGGGGTAAAGTTTGGGACTTATAGACTTCTTGTTCTGATGTCACGTCC690.13177495130056147No Hit
TGCTTGGGGCATGTCGGGACAGATGCAGATGCCATGAAGTACACCATTGTGG680.1298651693976548No Hit
TAGGATGGGAATGCAGGGACTAGAAGCCTCAGTTTCCCCATTTTACCCTTCC680.1298651693976548No Hit
TTTTGGGGAACAGAATCATATCCTTAAGGATGACCATCATTCCCCCAACTAA680.1298651693976548No Hit
CTTGAAGGGGTGCATAGAAAAGCTATTTAATATTTATCTTGTATCCAGGTAT670.12795538749474808No Hit
ATCCGGGGAAGTCTATGAACTGTTAAATCAGCATTCATTTTAAGAAAAGCAA670.12795538749474808No Hit
TACACTGGGGTCTGGCTGAAGATTTGATGTGGTTCCTCCTTAAGCTATGCGT660.12604560559184141No Hit
ATCAGGGGGAGACTCTGATAAGTTATGTATGTATAACAAAACACCTAGAGAA650.12413582368893472No Hit
CTTATGGGGGGCAGTTCTCCTCCACTGCCTTATCCTTCAGCTGGCCAGCCCA650.12413582368893472No Hit
ACACCAGGGATATTAGAAGTGGTAAAAATATACAAGTGTCTCAGTGTCAACC650.12413582368893472No Hit
CAAGTAGGGATCATTCAGGGGTGAAAAACAAAGAGCCGTTTTAATGATGTTG640.12222604178602804No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTC640.12222604178602804No Hit
GGTCACGGGTACGGCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACG640.12222604178602804No Hit
CACATAGGGAAACATTAAAAAGTACCGCATGTTGGAGAGGTTGTTTAGATAC630.12031625988312133No Hit
AGATTTGGGAAGGGACATCTGTATTTAACTCCTTTGTAGACATGAATTTCTA630.12031625988312133No Hit
CCATATGGGGGGGCACAGAGTGCCCACGTTAGCCCCGGGCTCTGATAGAGAG620.11840647798021466No Hit
CAAAATGGGATATTAGTCAAACCAGAATTCAGATTCTAAATTTATCTAATGG620.11840647798021466No Hit
CAACTAGGGCTGTTCAAAGGAAATTTTAATTTCAGTCTACCAGAAAAGTTAA620.11840647798021466No Hit
TAGAGTCGGGCTACGAAGGGGATTGCCAGAATCTTAGTAAATGTAATGTTTT610.11649669607730798No Hit
ACACTAGGGATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACA610.11649669607730798No Hit
ACCTAAGGGGAATGTATTTTCTTCTGTGCTTTTCACTTCTCAGTAGTTTGTT600.11458691417440128No Hit
TAGTGAAGGGATTGTTAGAAATAATTACATTAGTCTTGTACCATGAACATTA600.11458691417440128No Hit
AATTTGGGGATTTCTCAATCTGTGAGTAATGGACTAATTGCTATTAAATTTG590.11267713227149459No Hit
AGTTATGGGATGAAGGAGATGAAATAAGCATGATGCTGGTGCTGTCCAGACA590.11267713227149459No Hit
TTGCATGGGAACATCATAATTAATCATGTGGCTGTGAAATTCACAGTAATAT590.11267713227149459No Hit
ACACATGGGATTGAGATTTTTAAGATACATGCAAAGGTTTGGAAATAGAACC590.11267713227149459No Hit
ACAATCGGGCTATAATATATGATGGATTTTTTCCTAATTTTTTATATTTCCT590.11267713227149459No Hit
TTATCGGGGAGGGCCATGTCAGAGGACACCAGGGTGGCCAGGAGGAGAAGCC580.1107673503685879No Hit
AGTAAAGGGAACTCAGAATGAAAAAGCAATTCTGAGAAAACTATATGTGACA580.1107673503685879No Hit
CTTAAAGGGAGAGGGAGATACAGAAACCGACAGGGGCCAGGCGCCCGGTGGC580.1107673503685879No Hit
CAATTGGGGAAAAACATATCATGTAATTCAAAAACACTATTGATATTGAATA570.10885756846568122No Hit
TTCAAAGGGGGGGCATGAAGCACATTAATTTGCAGTGGCTGCTTCATATGAG570.10885756846568122No Hit
CGCTGAGGAAATTGACTTTGAATATCCTTTTAGACTCTTTTGTTTTAGAAAG570.10885756846568122No Hit
GACACCGGGGAAAGTGAAAAGCATGTTGAAGAATTCATCTTCTCACAGCACT570.10885756846568122No Hit
CTTTATGGGGGGATGAGAGTTTTGGATGCAGTGCTCGCTGTTGCTGAATAGG570.10885756846568122No Hit
GTATGCGGGAACCTAAAAACATGGAGCCAAGCTAAAAATGCCCTGGGGGTAA560.10694778656277454No Hit
AAACCAGGGTCACTATCCGTGATCCAGTCTTAATTTGAGCATGAGAGCAAAA560.10694778656277454No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACCTCGTATGCCGTCT560.10694778656277454No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCT550.10503800465986786No Hit
TTTTACGGGCGATCTGTTGTGGGGATATAATAAGTATTTATAGAGTCAAATC550.10503800465986786No Hit
CTTTAAGGGATAACAAAACATCTGGGCTTTATAAGTATGTAAAGAATAATGA550.10503800465986786No Hit
TATTAAGGGGGGCAGTCTTCCGAGCAAGATGGCGCCGCGGGCATTTCTTCCA550.10503800465986786No Hit
CTATTGGGGGGGGAGAGGAGCAAGTAAGGACACTGCGACCTTCCTCTCTGGG550.10503800465986786No Hit
CCGCCCGGGATCGATCAATTTGGGGAACACTGACATCTTTGTTATATTGAGT540.10312822275696115No Hit
GTCACGGGCCCAAGAGGGTCTGCTCTGAGCCTGCGTTCCTAGGTGGCTCGGC540.10312822275696115No Hit
GCCCGCGGGATGATAAAACTATACCCGATTAAAACGTTGACTCTATTAGAGG540.10312822275696115No Hit
TTTAATGGGCAATCTGATTTTCCCATTCTTACTACTTCACAGCCATGAATGT540.10312822275696115No Hit
GCCACGGGGTTGCGCGGGGATGACTAGTGGTCTTGATGCTAGAGTCAACTTA540.10312822275696115No Hit
ATGTCCGGGAGCTCTTCCCAGGAAGGGAGGAAGTTTCTGGAAGAGAAGGGAA530.10121844085405447No Hit
TACTTTGGGCTGAGCTACTAAGGAGACTGAGACGGGAGAATTGCTTGAACCC530.10121844085405447No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTAT530.10121844085405447No Hit
CTGCACATGGGGGGGAATGTTTGTTTTATATGCATATTGTATCCTGACTGTG530.10121844085405447No Hit
CACCTGGGGGAAAATAGGAATTCAGACACAAATAAAAGGATTTTTTAAATTA530.10121844085405447No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)