FastQCFastQC Report
Sat 31 Dec 2022
SRR5847422.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847422.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51903
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT25024.820530605167331No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC23324.492996551259079No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC4420.851588540161455No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC4280.8246151474866579No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT3020.5818546134134828No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA2860.5510278789280003No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGT1500.28900063580139873No Hit
TACACTGGGAGGAACTAAAATGTCTATAAAGGCTTCTTAGGGGAGCAAGAGA1370.2639539140319442No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAACTCGTATGC1360.26202724312660153No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTATACAC1320.2543205595052309No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAT1290.24854054678920293No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCC1250.24083386316783228No Hit
TACCTGGGGACTTTTAAAGATGAGTAAAATTCAGATCAGCAAAGATGGGGGT1210.23312717954646167No Hit
GCATGTGGGATGACTGAATGTGAGCTAAGCATTTTCTCCTGTGGAGCCCTAG1110.21386047049303508No Hit
TTTCGTGGGGGGGAGCCGGGGTAGTCGCCGCCGCCGCCGCCGCTGCAGCCAC1110.21386047049303508No Hit
TAAAAAGGGTGGAAGAGGAGTCTCTGGGCTCGGTGCCAGCGCCTGCCTGCGC1010.1945937614396085No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTA1000.19266709053426584No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTC980.18881374872358053No Hit
AACGCAGGGAGGTATGAGCTGTTTTAGAAGCAGCGTGTTGCCTTCATCTCTC960.1849604069128952No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCTGTCTCTTATA950.18303373600755254No Hit
CAAAAAGGGGCTGTAAGAATTTCTTAAAATCTGGCATATTTGTCCTTGTCAA940.1811070651022099No Hit
AGATCTGGGGGGCTGTGGAGAGAGCGCGGAGACAAGCGCAGAGCGCAGCGCA930.17918039419686724No Hit
TTTGCTGGGGTGGTCATGGTCACCCAGGAGAGTGACAGCAGCTTTCTGGTCA920.17725372329152458No Hit
AGTTATGGGATTGCGGCAGTGGTACCTTGCCTTCAAGACTTGGGAAATCTGA890.1714737105754966No Hit
ACCTAAGGGGAATGTATTTTCTTCTGTGCTTTTCACTTCTCAGTAGTTTGTT880.16954703967015394No Hit
ATGAAAGGGGGAAGTTAATACAAAAAAATTTCAAATTATTTCTATTGTAAAT870.16762036876481126No Hit
GCGAATGGGGACACTGGAGATTGCTTGAAGAGGAAGGGTGGTAGGGAGCTAT870.16762036876481126No Hit
TTATGCGGGGTCACACTTCCCGCCCCACACTCAACTGTTGTGTGAATGAGCC860.16569369785946864No Hit
AGGCATGGGATCTCTATTTTTCTTAGAGGTTTATTAATTTTATTGACCATTT850.16376702695412595No Hit
TAGGATGGGAATGCAGGGACTAGAAGCCTCAGTTTCCCCATTTTACCCTTCC840.1618403560487833No Hit
CTTAAAGGGAGAGGGAGATACAGAAACCGACAGGGGCCAGGCGCCCGGTGGC840.1618403560487833No Hit
GATGCAGGGGGGATAACCTCTGTGCGCCCCAGGGTTCAATGTTTGGGTGGAG830.15991368514344065No Hit
CCACTTGGGGTGTCAAAGAAGAAATTGAAAAGGAAACTAGAAAGTGTATAAG830.15991368514344065No Hit
CATTAGGGGCAAGCGAATAACTGTGACTATTTAGACAGCATTTTATTTTGTA820.157987014238098No Hit
CGACTGGGGGTCAAGACACCCTCTTCCTGAGGGCCAAGGATGAGGCTAGTGC810.15606034333275534No Hit
AATACTGGGAACATTTTAATTCTGTAAAAAGAATAGAACATGCTCACTGATC800.15413367242741266No Hit
GCGCGCGGGAAACTTATAGATAAATGATGACTAATGGGAACGTAATGCATGT800.15413367242741266No Hit
CTTGAAGGGGTGCATAGAAAAGCTATTTAATATTTATCTTGTATCCAGGTAT790.15220700152207No Hit
ATACTAGGGACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGA780.15028033061672735No Hit
AGATTTGGGAAGGGACATCTGTATTTAACTCCTTTGTAGACATGAATTTCTA770.1483536597113847No Hit
GTAATAGGGGAATTTTTGTCATGTAAAAATATTAAATTGCAAAATCAAAATC770.1483536597113847No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCA750.14450031790069937No Hit
ACAGTTGGGAAAACTCTGTCATATAAGTGACTACCATGGATGAAATAGGTGT740.14257364699535674No Hit
CGAAGTGGGATATCACTATATATTGATTTTTATTTTATATATTTCCATGTAA740.14257364699535674No Hit
AGTTATGGGATGAAGGAGATGAAATAAGCATGATGCTGGTGCTGTCCAGACA740.14257364699535674No Hit
ATCAGGGGGAGACTCTGATAAGTTATGTATGTATAACAAAACACCTAGAGAA730.14064697609001406No Hit
ACAAAAGGGATGGCGCCACTGCACTCCAGCCTGGCGACAGAGCAAGACTCAG730.14064697609001406No Hit
TACTTTGGGCTGAGCTACTAAGGAGACTGAGACGGGAGAATTGCTTGAACCC710.13679363427932875No Hit
GCATGAGGGGTTGTACCAGTTTCTTAATGATATTTTGTTACTTCTAGAATAT700.1348669633739861No Hit
TTAGCCAGGGGGAGATCATCGAGGCTATCTACAAAATGGTAGGCACTGTGAT690.13294029246864345No Hit
TCTTGTGGGGTAACCTAAAAAGTTTTGGCATATTTAGGTCAGCCTAGCGGAA690.13294029246864345No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACCTCGTATGC680.13101362156330076No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTC680.13101362156330076No Hit
CACATAGGGAAACATTAAAAAGTACCGCATGTTGGAGAGGTTGTTTAGATAC670.1290869506579581No Hit
TACACTGGGGTCTGGCTGAAGATTTGATGTGGTTCCTCCTTAAGCTATGCGT670.1290869506579581No Hit
CCACAAGGGGCAAAGAAGTAACCATGATTTCAGACAAAAATGTGAAACAGGA670.1290869506579581No Hit
CTTTATGGGGGGATGAGAGTTTTGGATGCAGTGCTCGCTGTTGCTGAATAGG670.1290869506579581No Hit
GTTAGCGGGACCTGGGGGTGTCCCAGGGACTCTGTCAGTGCCTTCAGCCCAC660.12716027975261546No Hit
ACTCATGGGAACTTCACTATAGTAGAAAATATCAACTACCATCCTTTTCTCT660.12716027975261546No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATG660.12716027975261546No Hit
ATCCGGGGAAGTCTATGAACTGTTAAATCAGCATTCATTTTAAGAAAAGCAA660.12716027975261546No Hit
TAGAGTCGGGCTACGAAGGGGATTGCCAGAATCTTAGTAAATGTAATGTTTT650.1252336088472728No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCTGTCTC650.1252336088472728No Hit
CAACTAGGGCTGTTCAAAGGAAATTTTAATTTCAGTCTACCAGAAAAGTTAA650.1252336088472728No Hit
TTTTGGGGAACAGAATCATATCCTTAAGGATGACCATCATTCCCCCAACTAA650.1252336088472728No Hit
AATGCCGGGAGGCTTGCCGGTTAAAGGTTTAACTGATTTCCATATACTTTCC640.12330693794193014No Hit
AGGCTTGGGGTTATAAGTGTGTAGGAGTTATAACTTATTTTAAGAAGCAGTA640.12330693794193014No Hit
GACGTGGGAAGGATATCTACGGCAACACTGCTCTCCATTATGCCGTGTATAA640.12330693794193014No Hit
AAGCTGGGGTAAAGTTTGGGACTTATAGACTTCTTGTTCTGATGTCACGTCC640.12330693794193014No Hit
TAACATGGGCTTATTCCTTTGTATGATAGAAGAGTAGATTTTAAAATATAAG630.12138026703658747No Hit
TTTAGCGGGCTCTGGGCCCAGAGGGAGAAGAGCTGGCGAGAGTGGCAGAGGG620.11945359613124483No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTAT620.11945359613124483No Hit
CTTATGGGGGGCAGTTCTCCTCCACTGCCTTATCCTTCAGCTGGCCAGCCCA610.11752692522590216No Hit
GTATGCGGGAACCTAAAAACATGGAGCCAAGCTAAAAATGCCCTGGGGGTAA610.11752692522590216No Hit
GTCATGGGGGTCCTCCATGGAGCCACTTTGCAGGAGCACACGTGTGTTCTGC600.11560025432055951No Hit
TTATCGGGGAGGGCCATGTCAGAGGACACCAGGGTGGCCAGGAGGAGAAGCC590.11367358341521684No Hit
CCGCCCGGGATCGATCAATTTGGGGAACACTGACATCTTTGTTATATTGAGT590.11367358341521684No Hit
AGCATCGGGGTGGCATGGCCCTGTTTCCTGTTGATATGGTGATAGTTGGAGA590.11367358341521684No Hit
CGTATTGGGATGTGAATATGGCTCTTGTAAAGGATTAACTATTGTAATTTTA590.11367358341521684No Hit
GTGTCTGGGGACTCTCATGTGACCAATCAATCCCTGTTGGAAGCTGGGCCCT590.11367358341521684No Hit
TGCTTGGGGCATGTCGGGACAGATGCAGATGCCATGAAGTACACCATTGTGG580.1117469125098742No Hit
ACACTAGGGATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACA580.1117469125098742No Hit
GTCAACGGGGGCTCACTGCAACCTCCACCTCCTGGGTTCAAGTGATTCTCCC570.10982024160453153No Hit
GGACACGGGGGTCACCAGAAAGACCAATTATGTAATTAGAAGAACTTTCAGC560.10789357069918887No Hit
AATTTGGGGATTTCTCAATCTGTGAGTAATGGACTAATTGCTATTAAATTTG560.10789357069918887No Hit
ACTAACGGGAACTCAAATGTCCTTCCATAGGTGACTAGATAAACTTTGTTAC560.10789357069918887No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACCTCGTATGCCGTCT560.10789357069918887No Hit
ACACATGGGATTGAGATTTTTAAGATACATGCAAAGGTTTGGAAATAGAACC560.10789357069918887No Hit
ATCTCGGGGTTACTGGAAAAATTAAGTTGTTGAAAGTAAGTTGTTTTCCCAT550.1059668997938462No Hit
TATTATGGGTACAAAATTTCGTGTTTCTTAGTGATTTTAAAATGCATGTATT550.1059668997938462No Hit
AAGCGCGGGGTTGTGCTGTGGGGAAGGGAGAAGGATTTGTAAACCCCGGAGC550.1059668997938462No Hit
CAGACTGGGAAGATTGGAGGGTAGGTGATGGTAGAAAATTCTGCGGCAAACA550.1059668997938462No Hit
GCATATGGGACAGCCAAAGAATGGAATGTATATTCACTCCACCCTCATTATA550.1059668997938462No Hit
GCCACGGGGTTGCGCGGGGATGACTAGTGGTCTTGATGCTAGAGTCAACTTA550.1059668997938462No Hit
CTGTCTGGGCATTCCCATACACAGAAACACAAAACACTTTGATCATAAACTT540.10404022888850356No Hit
TGGTATGGGGTTTGTCTTTTTCAATGAAGCTTTGCAGATCACCATGTGGTTG540.10404022888850356No Hit
AGTAAAGGGAACTCAGAATGAAAAAGCAATTCTGAGAAAACTATATGTGACA530.1021135579831609No Hit
AAACCAGGGTCACTATCCGTGATCCAGTCTTAATTTGAGCATGAGAGCAAAA530.1021135579831609No Hit
CTTTAAGGGATAACAAAACATCTGGGCTTTATAAGTATGTAAAGAATAATGA530.1021135579831609No Hit
GTCAAGGGGACTCACACATTCTTGGGGAAAAAAATCAAATGTCAGTCCTAGC530.1021135579831609No Hit
TAGATAGGGGGAAGGCTCTCTTTTCATCAGATCGCTTAGAAAATGGCCACAG530.1021135579831609No Hit
TATCAAGGGTCTGGAAAGAAAGGTGACTTTAATCATTGCTGCCTATACATGC520.10018688707781824No Hit
CCCAAAGAGCCATTTCAACAGAGAAGGGAAATGACAAAGGGGCAGCTGGCCA520.10018688707781824No Hit
CTCTTTTAGGGAGATGATAATATGCTAAATTTTCCTCTATCCTGTACATTTC520.10018688707781824No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)