FastQCFastQC Report
Sat 31 Dec 2022
SRR5847421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176959
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT48322.730576009132059No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC36122.0411507750382856No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC7070.3995275741838505No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT4470.25260088495075134No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC3280.1853536694940636No Hit
CTCTTGGGGGCACCATGGATGGCCCTCATTAAAAATTAGAACTTTCTATTAA2440.13788504681875463No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTATACAC2430.1373199441678581No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA2410.13618973886606503No Hit
GTACCAGGGATGGTGATGATAGAGAAGAAAATGGGATTCTGAAGGATTTTTC2020.11415073548110015No Hit
CATAGAGGGAAGAGCTGAAAGACCTTCCTTATGGTGATTCCTTAGGATTCTT1900.10736950367034172No Hit
TGTGCAGGGACTAAAAACATTGCTGCAATTACTATTGCTGCATAATAAGTCA1850.10454399041585904No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAACTCGTATGC1770.10002316920868674No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)