FastQCFastQC Report
Sat 31 Dec 2022
SRR5847420.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847420.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173537
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT47242.7221860467796493No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC35802.0629606366365674No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC6780.3906947797875957No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT4370.2518194967067542No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC3300.19016117600281207No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTATACAC2440.14060402104450348No Hit
GTACCAGGGATGGTGATGATAGAGAAGAAAATGGGATTCTGAAGGATTTTTC2340.1348415611656304No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA2110.12158790344422227No Hit
AGAACAGGGAAGCACACAGACCAAGATTTCTGATGGTGACACAAAGATTGTT1890.10891049171070147No Hit
CTCTTGGGGGCACCATGGATGGCCCTCATTAAAAATTAGAACTTTCTATTAA1880.10833424572281415No Hit
CATAGAGGGAAGAGCTGAAAGACCTTCCTTATGGTGATTCCTTAGGATTCTT1850.10660550775915222No Hit
CTTCAAGGGGTAGAAACAAGACAGTTAAAGGAGCACAGGAATTCACTGAAGA1800.10372427781971567No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)