FastQCFastQC Report
Sat 31 Dec 2022
SRR5847419.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847419.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173316
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT43762.5248678714025248No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC34902.013662904752014No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC5780.3334948879503335No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT4520.26079531030026076No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC3220.18578780955018578No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA2280.13155161670013155No Hit
GTACCAGGGATGGTGATGATAGAGAAGAAAATGGGATTCTGAAGGATTTTTC2110.12174294352512173No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAACTCGTATGC2060.11885803965011885No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTATACAC2000.1153961550001154No Hit
CTCTTGGGGGCACCATGGATGGCCCTCATTAAAAATTAGAACTTTCTATTAA1930.11135728957511136No Hit
CATAGAGGGAAGAGCTGAAAGACCTTCCTTATGGTGATTCCTTAGGATTCTT1830.10558748182510559No Hit
CTTCAAGGGGTAGAAACAAGACAGTTAAAGGAGCACAGGAATTCACTGAAGA1750.10097163562510098No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)