FastQCFastQC Report
Sat 31 Dec 2022
SRR5847418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85451
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT27693.240453593287381No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC25432.975974535113691No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC5500.6436437256439362No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC4120.4821476635732759No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT3510.4107617230927666No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA2830.3311839533767891No Hit
CTTCAGGGGGTCAAGGTTATAGTGAGCTATGATTGTACCATTGCACCCCAGC2260.26447905817369016No Hit
AAATACGGGATTAATCAAAGTTCTTGTCTTCTGATAACTGAATACCCCACCA1890.22117938935764356No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAT1800.21064703748347008No Hit
ATACATGGGTATCAATAGAAACCTACAAAACTGACAAGCTAAGAGAGCAAAG1710.20011468560929657No Hit
AAGCTTGGGGGAATTTGTAATAAGTTCATTTAATATACTCTTGCTCCAGGGA1710.20011468560929657No Hit
CTTGTGGGGGAGGTCACTGACTTAACCAAAGACACCAGGTAGTATATGTTAT1630.19075259505447567No Hit
GTAATAGGGACATGGAACCACAAAATACCAAAATAGCCAAAACTATCCTGAG1490.1743689365835391No Hit
ACAGTAGGGTGAGCAACTATATACCAAAAAGTTAGAAAACCCAGAAGAAATG1450.16968789130612866No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTATACAC1430.16734736866742345No Hit
GCATCGGGGAGAGGACAAATAGTTTATTTAAAGCATTTGTTCCAATAAAATA1430.16734736866742345No Hit
TCACTCGGGCCATATATATAATATGAATTGTGATCACTGCTACTTTTATAAA1330.1556447554738973No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAACTCGTATGC1310.1533042328351921No Hit
ATCCCAGGGCCTTTGTCCTGCCTTTCCCTCAGCACTAGCTATTGTACTTATC1290.15096371019648688No Hit
AGCATTGGGTGTCTGGAGACAAAGAGAAAGATGAAGGGTAGGAATATGCAAT1280.14979344887713428No Hit
ACGACTGGGGCTGTAAGATCAGAGAAGTTTATAATAATTGAAAACCAGTATT1270.14862318755778167No Hit
TATAGCGGGGTTTGGAACTCTGAAGGATTTAGACAAGGTTTTGAAAAGGATA1270.14862318755778167No Hit
CTACCGGGGTCTTGTAACGGGACAGTTCAGGATGTTTTTGCAATTTTCCCTC1270.14862318755778167No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCC1270.14862318755778167No Hit
TTGTAAGGGGGTGAGAGGAGGCTGCAACGCCGAGCGGAGGAGGCAGGAACCG1270.14862318755778167No Hit
ATGGACGGGTCCTGGCCAGCCTGCAGTCATGCGAGAGCAGTAAAAGCAAGTC1260.14745292623842904No Hit
CTACAAGGGCAGGAATTTTAATAAGATTTTGTAAAGAATATCCAGAAAAGTA1240.14511240359972383No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGT1220.1427718809610186No Hit
GCACCGGGGTTTCTAATATATGCATTCATACTATAAATTTTCCTCTAAGCAC1210.14160161964166598No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTA1210.14160161964166598No Hit
AATCTGGGGGTCTTCAGGATTTGTTAATAAAAGATAATCTTTCTAGAAATAG1160.13575031304490293No Hit
ACTCTAGGGGATATTCTACATAGCATATTGTTTCCCTGTTTAAACCTTAATT1160.13575031304490293No Hit
TTAACGGGGCTATCAATGTTTGGATCATCTCAGTGGGGGAAATGACTTAATT1140.1334097904061977No Hit
TAGCATGGGAACTTTTAGCATGCTGCACAGAAACTGGTATAACATGCCTTCA1140.1334097904061977No Hit
AAGAATGGGATGTTATAACCAGTATCACTAAAAAAAATCATTGACAGATTTA1130.13223952908684508No Hit
ACGGCCGGGATCTGAAACAGGGACTTTGATAAAGGATTAGTAGACATATCAC1120.13106926776749248No Hit
TATATAGGGGTATTTGAGAAATGAATGAGATGACTTAATGTAGTCTTAACAT1100.12872874512878726No Hit
AACGGTGGGAGAACATAGGTTCTCAGTATCTAGAACTTACATCATTATCATC1100.12872874512878726No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACCTCGTATGC1090.12755848380943466No Hit
ACTTCCGGGAACTTTTCTGCAAGAAATACAAACTTACATCTTCCTTTTACTA1090.12755848380943466No Hit
ACTATAGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGTG1090.12755848380943466No Hit
TGGACAGGGGTAGCTATTTTTTGTTGCTTGGCTTTTGAATGAGTGTAAATTG1070.12521796117072942No Hit
CCGCGTCGGGGAGTGCACTGTGCCACGGAACGACATGCTGACCAGGGTTGGT1060.12404769985137681No Hit
ATCTCTGGGAATAGTGGGAGACTTTAACACCCCACTGTCCATATTAGACAGA1050.12287743853202421No Hit
GTGTACGGGGTGCCTGAGGCACCCATTTCCCTACGTAGCAGCAGCTGGTGAC1050.12287743853202421No Hit
TTCGACGGGGGGGAAAGTAAGTCTTTAACAAAGGCTTTTGAAATAAATGCAT1050.12287743853202421No Hit
CAATAAGGGAATTTTTGAGAGGATGAAAATTTAAAAAGGAAAGGTCGATTAT1040.1217071772126716No Hit
CTTAACGGGGTGCCTACTGCCCCTCTAATGTATAGGGGGAAATGTATTCCTA1040.1217071772126716No Hit
GGTTTAGGGCTGAGACCTTAATGGACAGAATCAAGAAACAGCTACGTGAATG1040.1217071772126716No Hit
TGCAGGGTCTGTAGGTGGGATTCCAATTCAGAATGCATCACAAAAACGTAAA1040.1217071772126716No Hit
TTACAAGGGAAAGTTGGGATATTTGACTTTCATTGTCCTGAGTAAAATAGTG1030.12053691589331897No Hit
CTATAAGGGCAGTATATGTCAGTTCAACTTGAGCATTCTTTTGGATTCTTTG1020.11936665457396636No Hit
CGTAGAGGGATATCAATAGTAATTACTCTGTTGTCACATGTTTGAATGTATT1020.11936665457396636No Hit
TATCTTGGGACATTTGGCTGCATGAGCAAGTCGGCCGCACACTTCCAGACAG1010.11819639325461376No Hit
TTTGCTGGGGATGGCCAAGTGACTGCCTCAAGTAACACAGTAACTGGATGGA1010.11819639325461376No Hit
AATGTTGGGCATGAATCTTGAGTACGTGGACCATTGCTTGCATGTTAACTTT1010.11819639325461376No Hit
TGGTCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1000.11702613193526115No Hit
TTAGAGGGGAACAACGAGATTGAATAAATAATGAAAAACTGCCCACAAAGAA1000.11702613193526115No Hit
GCCTGCGGGCATATTTGTAGTCTATCACGAACTGGAACATTATTATGCAATG1000.11702613193526115No Hit
CATTGCGGGGTAGGTAAGACAGATTTAGAGATTGAGTCCCATAAAGTTAGAG1000.11702613193526115No Hit
AACATTGGGGGATGTGATGTGTTACTTCACTTTTTTGTTTTCTGTTCTTGAG990.11585587061590853No Hit
GCTGATGGGGGAACCTACTAGCTAACATAGGTGGAAACCATTCCTGTGGGCT990.11585587061590853No Hit
GCGAACGGGATAAGTTTCTGAAATAAACACTTTGTTATCACTTTTGAATTGA990.11585587061590853No Hit
GATAGTGGGATATCCAGCACACCGAGAAGGTTGATATTGAGAACTGGGACAG980.11468560929655593No Hit
TTTGGTGGGGAATAAATGCAGTAATCCTCTCCCAAATAAGCACACACATTTT980.11468560929655593No Hit
CATTGTGGGGTAGACTCTGTGGGTCTTCAAATTCTCTACTACACTTAGAAAG980.11468560929655593No Hit
TTCACAGGGATAGCATCATGTCACCTTAGTGGAAGATGCTCATCTTTTATTT980.11468560929655593No Hit
CAATCTGGGCTCTCTCTCCTGGAGCTGAGACATCATCTTCTTCTGCCCTTCA980.11468560929655593No Hit
ATAAGCGGGGGAGAATAAATGCAGTAATCCTCTCCCAAATAAGCACACACAT970.11351534797720332No Hit
CTCACTGGGGCAGGCGCAAGCCGGCAAGATGGCGGCGGCTGGGGCTGGCCGT970.11351534797720332No Hit
TTCCCTGGGAAGGAAAAATCTTAAATAAGAATTGGGTTTTAATTTTCCATGA970.11351534797720332No Hit
AACAACGGGACGCTCTTCCGATCTTTCGCTGGGAAGTTGATAAAGAATTATC970.11351534797720332No Hit
AAGAATGGGGAGGAATGCTGAAAACTAAATTTTAAGTATCAAGTCTAGACCA960.11234508665785069No Hit
GGGGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA960.11234508665785069No Hit
ATATTGGGGCTAGCCAAGGGATAGTTCTCCCCTTTGAGTGCATTTAACTCTT950.11117482533849808No Hit
TACAAAGGGCGATCTGAAATTGGGGTTTTAAGAAAGTCATTATATTTCTGTT950.11117482533849808No Hit
TAAGAGGGCTTCTGTATTTGGGGCCTATTTATGCCACCAGTAATTTTATAAG940.11000456401914548No Hit
TGTTCCGGGAGGCAGCGCATATGGTTTGTCGGGCGCCGATTCCTACAAAGAT940.11000456401914548No Hit
TACATTGGGAATAGAAACCTTGCCATTTGTATGTACATATTTTAAATTAACC940.11000456401914548No Hit
CGAATAGGGGAATTCTTGTGAGTTATTTTATTGTAGTATTTTTCTCTTAGTT940.11000456401914548No Hit
TGTGCTGGGTTTATGGTTAAATGCAGTAATTATGGAACATTTTAAAGTGAAT940.11000456401914548No Hit
ACTTGCGGGGTGAATGCAAATGTTCCCGATAAATTGTTAAGAAATTAATACA930.10883430269979287No Hit
CGGTTCGGGTCGATAACAATCTGTTGGGATGGGTTATGATACCAGTTGTGAA930.10883430269979287No Hit
ATGGCCGGGAACACATACACTATGATTAGCAGCTGAATTTAACAGTATCTTT930.10883430269979287No Hit
CTTGCAGGGTACCTAAGTCCTCTGGCACTCGTGACTAGGCATTCAATAAGTA930.10883430269979287No Hit
GAGTGCGGGGATTGCCTGTTGTTCGGTGTTGTGAATAGCACCTCTGAATAAG930.10883430269979287No Hit
CTATAGGGATACTTATCTGATTATTTAGCACCTTATGGTAGGACCGTGCTGT930.10883430269979287No Hit
TGGAGTGGGATCATAAATATTTGAAGTTTATTGTTACTCAAACTTTCTTCTT930.10883430269979287No Hit
CAGCAGGGGATTGGTGAGGCTTTTATTTCCCTTGAGGAGTCTCTTGTACCTA920.10766404138044025No Hit
AGGCATGGGTGGCTATTTTATTTATGTGAGACTCTAAACCTGATTGTCCTAA920.10766404138044025No Hit
TTTAATGGGGGGTTATTCTGTGGCTGTCGGAGATTTCAATGGTGATGGCATA920.10766404138044025No Hit
GTTACTATGGGGTTTTGATTGGTGAGAAAGTACCCATTCTCTTCAAATAATC920.10766404138044025No Hit
AACTGGGGAGTAGTTTTCCTTATAAGGAGCATGTATCACTTAGCAAGAAACT920.10766404138044025No Hit
TTACTTCCAGCCAAGAATGATATCCAGAAAACACTTGATGACATTGTTGGGA920.10766404138044025No Hit
GCACTAGGGACTGTGATGTCTGGTGCTTGAGAATGAGAAATGAGGTTTTAAC920.10766404138044025No Hit
GACTTGGGGCTAAATAAAAATTAACAGAACAGGAAATCTGTTACAGAAATTT910.10649378006108765No Hit
ATGTTAGGGATCGGGAAGCTCTTGCTGGGAAGCTGGAAACACATTGGGTAGC910.10649378006108765No Hit
AAATGCGGGATCCTCTGCTGGTGCTAAGTGTGATTAGGAAGAAGCCTGGGGA910.10649378006108765No Hit
GTAACCGGGGGTCTAGATAACTGCTGTGTGAATCAAGACAGTAAAATTGTGT900.10532351874173504No Hit
TCAAAGGGCTAAGGCAGGGGGTCTATTAAGAGATTCAACTTCTTCCTGGTTT900.10532351874173504No Hit
ATCTCTGGGACAATAGTAGCTTCTCTCAGATTTCTCATAATCGTTGGTTGTC890.10415325742238243No Hit
GCAATAGGGATTTTTAAAAGTATTATGTTGATACCAGTGTTTTAATTTGAAT890.10415325742238243No Hit
GTAGTAGGGATTTGGAGAATGTTTGCTAGTGAAAAACACTTTTGTCCTGTGA890.10415325742238243No Hit
ATGCTGGGATTACAGGCATGAGCCACCGCGCCTGGCCTAGATACCTGTTTTA880.1029829961030298No Hit
CACACGGGGAGACTTTAAAATGTAAAATAAAGATGTATGCTGCCTGTCAGCT880.1029829961030298No Hit
GTAGGCGGGATATGAGAGTTTCTCTGGCTCTATATCCTCACTAACACTTGTT880.1029829961030298No Hit
CTGCTGCGGGGCATCTTTCAGTTTGTGTATATTCTATATATACGACTTCACC880.1029829961030298No Hit
GTGCCGGGGTATATTGTCATTATTTTTGAAGTATAAGGTATTTTTAATTTGT880.1029829961030298No Hit
AGCAATGGGGTGGGAGGGCGGTGGTGAGGGAAGTATCTCCCCAAAGGAAAGA880.1029829961030298No Hit
AATATGGGGAACAACAAAGCATGCTCAGTATTGAGACACTGTCAAGATTAAG870.1018127347836772No Hit
CTCAGCGGGGAACACCCACTCTGTGTCGGGTTTTGGAGACATAAAGGCAAAT870.1018127347836772No Hit
CTTCAAGGGAGGTCTGGCCTTAGACAGGAGTTTGGGGATGGGCGCGGTGGCT870.1018127347836772No Hit
TTAAGAGGGATATACAACTTTTTAAAAATCTATTTTATTGCTTAAGCCATGA860.10064247346432459No Hit
GTCAAAGGGACTCTTTCTCTTTTATGTTATATGGTTAGTCAGATAGGACCCG860.10064247346432459No Hit
GAAAGGGATGAGAGAAATGCAAATCAAAACCACAATGAGATACCATCTCACA860.10064247346432459No Hit
ACACATGGGTAGTTTTAAAATAATGATCTCAGATTTTTAAAAAGGATATAGG860.10064247346432459No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)