FastQCFastQC Report
Sat 31 Dec 2022
SRR5847416.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847416.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84415
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT27513.2588994846887402No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC25052.9674820825682637No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC5600.6633892080791328No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC3910.4631878220695374No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT3000.3553870757566783No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA2440.289048154948765No Hit
CTTCAGGGGGTCAAGGTTATAGTGAGCTATGATTGTACCATTGCACCCCAGC2140.25350944737309716No Hit
AAATACGGGATTAATCAAAGTTCTTGTCTTCTGATAACTGAATACCCCACCA1980.23455546999940768No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAT1720.20375525676716222No Hit
AAGCTTGGGGGAATTTGTAATAAGTTCATTTAATATACTCTTGCTCCAGGGA1700.20138600959545105No Hit
ATACATGGGTATCAATAGAAACCTACAAAACTGACAAGCTAAGAGAGCAAAG1630.19309364449446187No Hit
CTTGTGGGGGAGGTCACTGACTTAACCAAAGACACCAGGTAGTATATGTTAT1500.17769353787833914No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGT1490.17650891429248355No Hit
TCACTCGGGCCATATATATAATATGAATTGTGATCACTGCTACTTTTATAAA1440.1705857963632056No Hit
TTGTAAGGGGGTGAGAGGAGGCTGCAACGCCGAGCGGAGGAGGCAGGAACCG1440.1705857963632056No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAACTCGTATGC1420.16821654919149442No Hit
ATCTCTGGGAATAGTGGGAGACTTTAACACCCCACTGTCCATATTAGACAGA1370.16229343126221643No Hit
ATCCCAGGGCCTTTGTCCTGCCTTTCCCTCAGCACTAGCTATTGTACTTATC1340.15873956050464966No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTATACAC1310.15518568974708286No Hit
TTACAAGGGAAAGTTGGGATATTTGACTTTCATTGTCCTGAGTAAAATAGTG1310.15518568974708286No Hit
CTACCGGGGTCTTGTAACGGGACAGTTCAGGATGTTTTTGCAATTTTCCCTC1290.1528164425753717No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTA1290.1528164425753717No Hit
ATGGACGGGTCCTGGCCAGCCTGCAGTCATGCGAGAGCAGTAAAAGCAAGTC1280.15163181898951608No Hit
TATAGCGGGGTTTGGAACTCTGAAGGATTTAGACAAGGTTTTGAAAAGGATA1270.15044719540366047No Hit
GTAATAGGGACATGGAACCACAAAATACCAAAATAGCCAAAACTATCCTGAG1270.15044719540366047No Hit
TATCTTGGGACATTTGGCTGCATGAGCAAGTCGGCCGCACACTTCCAGACAG1230.14570870106023812No Hit
AGCATTGGGTGTCTGGAGACAAAGAGAAAGATGAAGGGTAGGAATATGCAAT1220.1445240774743825No Hit
CCGCGTCGGGGAGTGCACTGTGCCACGGAACGACATGCTGACCAGGGTTGGT1210.14333945388852692No Hit
ACTATAGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGTG1210.14333945388852692No Hit
AAATGCGGGATCCTCTGCTGGTGCTAAGTGTGATTAGGAAGAAGCCTGGGGA1210.14333945388852692No Hit
GCATCGGGGAGAGGACAAATAGTTTATTTAAAGCATTTGTTCCAATAAAATA1210.14333945388852692No Hit
GGTTTAGGGCTGAGACCTTAATGGACAGAATCAAGAAACAGCTACGTGAATG1200.14215483030267131No Hit
AACTGGGGAGTAGTTTTCCTTATAAGGAGCATGTATCACTTAGCAAGAAACT1200.14215483030267131No Hit
TAGCATGGGAACTTTTAGCATGCTGCACAGAAACTGGTATAACATGCCTTCA1200.14215483030267131No Hit
CAATAAGGGAATTTTTGAGAGGATGAAAATTTAAAAAGGAAAGGTCGATTAT1190.14097020671681573No Hit
GAAAGGGATGAGAGAAATGCAAATCAAAACCACAATGAGATACCATCTCACA1190.14097020671681573No Hit
ACGGCCGGGATCTGAAACAGGGACTTTGATAAAGGATTAGTAGACATATCAC1180.13978558313096015No Hit
AATCTGGGGGTCTTCAGGATTTGTTAATAAAAGATAATCTTTCTAGAAATAG1180.13978558313096015No Hit
AATGTTGGGCATGAATCTTGAGTACGTGGACCATTGCTTGCATGTTAACTTT1180.13978558313096015No Hit
ACTCTAGGGGATATTCTACATAGCATATTGTTTCCCTGTTTAAACCTTAATT1180.13978558313096015No Hit
CTACAAGGGCAGGAATTTTAATAAGATTTTGTAAAGAATATCCAGAAAAGTA1170.13860095954510454No Hit
ACGACTGGGGCTGTAAGATCAGAGAAGTTTATAATAATTGAAAACCAGTATT1160.13741633595924896No Hit
GCACCGGGGTTTCTAATATATGCATTCATACTATAAATTTTCCTCTAAGCAC1150.13623171237339335No Hit
TGGTCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1120.13267784161582657No Hit
AACATAGGGATGTTTCTTAATAATTACTGGAGGTATGCAAATCTTATTTACA1120.13267784161582657No Hit
GGGGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1110.131493218029971No Hit
ACAGTAGGGTGAGCAACTATATACCAAAAAGTTAGAAAACCCAGAAGAAATG1100.13030859444411538No Hit
AACAACGGGACGCTCTTCCGATCTTTCGCTGGGAAGTTGATAAAGAATTATC1100.13030859444411538No Hit
TACCGGGTTCACGGGTGTGGGAGCCACGCTGGAAAAAAGATGGAAGTTGTGT1090.12912397085825977No Hit
AACGGTGGGAGAACATAGGTTCTCAGTATCTAGAACTTACATCATTATCATC1090.12912397085825977No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCC1090.12912397085825977No Hit
TTCGACGGGGGGGAAAGTAAGTCTTTAACAAAGGCTTTTGAAATAAATGCAT1090.12912397085825977No Hit
TGGACAGGGGTAGCTATTTTTTGTTGCTTGGCTTTTGAATGAGTGTAAATTG1080.1279393472724042No Hit
TTAGAGGGGAACAACGAGATTGAATAAATAATGAAAAACTGCCCACAAAGAA1070.12675472368654858No Hit
TTAACGGGGCTATCAATGTTTGGATCATCTCAGTGGGGGAAATGACTTAATT1060.125570100100693No Hit
TTTAATGGGGGGTTATTCTGTGGCTGTCGGAGATTTCAATGGTGATGGCATA1060.125570100100693No Hit
TTCCCTGGGAAGGAAAAATCTTAAATAAGAATTGGGTTTTAATTTTCCATGA1050.1243854765148374No Hit
GCCTGCGGGCATATTTGTAGTCTATCACGAACTGGAACATTATTATGCAATG1040.12320085292898181No Hit
AACGCTGGGCACCACCACTGAAGAGTTTCATATACACCAAATAATGTCTCAT1030.12201622934312623No Hit
CGTAGAGGGATATCAATAGTAATTACTCTGTTGTCACATGTTTGAATGTATT1030.12201622934312623No Hit
AAGAATGGGGAGGAATGCTGAAAACTAAATTTTAAGTATCAAGTCTAGACCA1030.12201622934312623No Hit
CTATAAGGGCAGTATATGTCAGTTCAACTTGAGCATTCTTTTGGATTCTTTG1020.12083160575727062No Hit
CTCTCTGGGAAGGCAAGTTTACATCACAAATCCAAGAATGTTTGCATCCTAA1010.11964698217141505No Hit
GACTTGGGGCTAAATAAAAATTAACAGAACAGGAAATCTGTTACAGAAATTT1000.11846235858555944No Hit
ATGTCTGGAGGGCAAATACAAATTAGAAATACCAGACTTAAATTATGAAACT990.11727773499970384No Hit
TACATTGGGAATAGAAACCTTGCCATTTGTATGTACATATTTTAAATTAACC980.11609311141384826No Hit
TGTTGAGGGGAATATATGTTTTGTCTCGCTATACTGCACTTACGCTATCCAG980.11609311141384826No Hit
CGAATAGGGGAATTCTTGTGAGTTATTTTATTGTAGTATTTTTCTCTTAGTT980.11609311141384826No Hit
ATAAGCGGGGGAGAATAAATGCAGTAATCCTCTCCCAAATAAGCACACACAT970.11490848782799265No Hit
GATAGTGGGATATCCAGCACACCGAGAAGGTTGATATTGAGAACTGGGACAG970.11490848782799265No Hit
CGGTTCGGGTCGATAACAATCTGTTGGGATGGGTTATGATACCAGTTGTGAA970.11490848782799265No Hit
CATTGTGGGGTAGACTCTGTGGGTCTTCAAATTCTCTACTACACTTAGAAAG970.11490848782799265No Hit
TTCACAGGGATAGCATCATGTCACCTTAGTGGAAGATGCTCATCTTTTATTT970.11490848782799265No Hit
ACTGCTGGGCCAAAGCCTAGATTTTATTCATAGGCCTCTTTAGGACATCTCT960.11372386424213707No Hit
AAGAATGGGATGTTATAACCAGTATCACTAAAAAAAATCATTGACAGATTTA960.11372386424213707No Hit
ACACATGGGTAGTTTTAAAATAATGATCTCAGATTTTTAAAAAGGATATAGG960.11372386424213707No Hit
TGTGCTGGGTTTATGGTTAAATGCAGTAATTATGGAACATTTTAAAGTGAAT960.11372386424213707No Hit
TGCAGGGTCTGTAGGTGGGATTCCAATTCAGAATGCATCACAAAAACGTAAA950.11253924065628147No Hit
ACTTCCGGGAACTTTTCTGCAAGAAATACAAACTTACATCTTCCTTTTACTA950.11253924065628147No Hit
TAAGCTGGGACTCCTTAAAGGCCATAAATGAGAGATATTTCACTTGCTATGA950.11253924065628147No Hit
GTAACCGGGGGTCTAGATAACTGCTGTGTGAATCAAGACAGTAAAATTGTGT940.11135461707042586No Hit
ACGCTAGGGTGGAGGAGGAATGCCTTCTCCTAAGAGTCAGTTGAAAGAAAGA940.11135461707042586No Hit
GTAGTAGGGATTTGGAGAATGTTTGCTAGTGAAAAACACTTTTGTCCTGTGA940.11135461707042586No Hit
CTCAGCGGGGAACACCCACTCTGTGTCGGGTTTTGGAGACATAAAGGCAAAT930.11016999348457028No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTC930.11016999348457028No Hit
AGGCATGGGTGGCTATTTTATTTATGTGAGACTCTAAACCTGATTGTCCTAA930.11016999348457028No Hit
AATTAGGGAGGTTTATGTATTGCCCATTTGGGGTAGATTTAGGAAAATATTT930.11016999348457028No Hit
GCGAACGGGATAAGTTTCTGAAATAAACACTTTGTTATCACTTTTGAATTGA930.11016999348457028No Hit
ATGTTAGGGATCGGGAAGCTCTTGCTGGGAAGCTGGAAACACATTGGGTAGC910.10780074631285907No Hit
TCGCTTGGGGGTCTTACACAGAAAGATACATCAAAAGCAGCATGACTCAAAA900.10661612272700349No Hit
ATCCGGGAATTACAAAAAAATTATTTCTACACTGACAGGATTTACTAGTTAA900.10661612272700349No Hit
TAAGAGGGCTTCTGTATTTGGGGCCTATTTATGCCACCAGTAATTTTATAAG900.10661612272700349No Hit
ATGGCCGGGAACACATACACTATGATTAGCAGCTGAATTTAACAGTATCTTT900.10661612272700349No Hit
TGTTATGGGGTCATGCATACTATACTTGGCAAACATCACCAAACAATGTTAT900.10661612272700349No Hit
GTGCCGGGGTATATTGTCATTATTTTTGAAGTATAAGGTATTTTTAATTTGT900.10661612272700349No Hit
AGTAGTGGGACTTGCTGATATTTTGTAGTTTGGAGATCCTTGTGGGCATTAT890.1054314991411479No Hit
GACTAAGGGATTCCTGATCTTCTTGCCTTTGCCTTTTATTAGAGGGGTGAGG890.1054314991411479No Hit
GTGTACGGGGTGCCTGAGGCACCCATTTCCCTACGTAGCAGCAGCTGGTGAC890.1054314991411479No Hit
TCAAAGGGCTAAGGCAGGGGGTCTATTAAGAGATTCAACTTCTTCCTGGTTT890.1054314991411479No Hit
AACATTGGGGGATGTGATGTGTTACTTCACTTTTTTGTTTTCTGTTCTTGAG890.1054314991411479No Hit
ACATTTGGGCACTGAAATTTTCCAGCTGGGCCACCTTGATGCCCGTGCTAAA890.1054314991411479No Hit
CTATAGGGATACTTATCTGATTATTTAGCACCTTATGGTAGGACCGTGCTGT890.1054314991411479No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATG890.1054314991411479No Hit
ACCTCAGGGCAAATTACTTTCTTTATAAATTGGGTCAGATTTAAAATAACTC890.1054314991411479No Hit
CAATCTGGGCTCTCTCTCCTGGAGCTGAGACATCATCTTCTTCTGCCCTTCA890.1054314991411479No Hit
TGCGTTGGGTGCACCGGGATTTTCGAAATACCAGTATTTCGACTCCAGGGGG890.1054314991411479No Hit
AGACACGGGGGCAGAAGGGGGAAGGCAAGTGAGCTCAGGAGACAGAGAGAGG880.10424687555529231No Hit
CAGAAAGGGAATGAACCTATATCTGAAAGCAGAGAAACCCATGCATCATGAG880.10424687555529231No Hit
GATCGTGGGCAACACATTTGGAATACAAGTGAATGAAAGTAACATAATTATA880.10424687555529231No Hit
CTGTATGGGGTGACCAACCGACTACTTCAGAGGAAACAAATGGTCATTGATG880.10424687555529231No Hit
TTACTTCCAGCCAAGAATGATATCCAGAAAACACTTGATGACATTGTTGGGA880.10424687555529231No Hit
TGGAGTGGGATCATAAATATTTGAAGTTTATTGTTACTCAAACTTTCTTCTT880.10424687555529231No Hit
TATATAGGGGTATTTGAGAAATGAATGAGATGACTTAATGTAGTCTTAACAT870.1030622519694367No Hit
GAGGTAGGGATGGGGTGCTTTACTTAAACCAAGGTGCCTCGGGGCCAGTGCT870.1030622519694367No Hit
GTTACTATGGGGTTTTGATTGGTGAGAAAGTACCCATTCTCTTCAAATAATC870.1030622519694367No Hit
CTTATAGGGAAGGGCTAGAACATTCAAACTGCAGCACCTTCTGCAGGGGATA870.1030622519694367No Hit
TTAAGAGGGATATACAACTTTTTAAAAATCTATTTTATTGCTTAAGCCATGA860.10187762838358111No Hit
ACTTGCGGGGTGAATGCAAATGTTCCCGATAAATTGTTAAGAAATTAATACA860.10187762838358111No Hit
AATAACGGGCAGTTTGGATTCTGAAAACTAACTGGCATCAACACTGGGTGTA860.10187762838358111No Hit
TTCTACGGGGAAGAACTGATTTGTAGAGTCAATGAACCTGTGTCCAGAATTT860.10187762838358111No Hit
CCTAGAGGGCGATCTTTTAGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGC860.10187762838358111No Hit
CTGCTGCGGGGCATCTTTCAGTTTGTGTATATTCTATATATACGACTTCACC860.10187762838358111No Hit
CAGCAGGGGATTGGTGAGGCTTTTATTTCCCTTGAGGAGTCTCTTGTACCTA850.10069300479772553No Hit
TGCTTGGGGATCGTTATGAATACCAGACCATTTGTAAGTGTGGTTGAAGAGC850.10069300479772553No Hit
GCTAATGGGGCAGAACAAAAAAGTAATTGAAGAAAAGGCTAGGATGATATAT850.10069300479772553No Hit
CATTGCGGGGTAGGTAAGACAGATTTAGAGATTGAGTCCCATAAAGTTAGAG850.10069300479772553No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)