FastQCFastQC Report
Sat 31 Dec 2022
SRR5847414.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847414.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84013
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT24252.8864580481592137No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC18872.246080963660386No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC4340.5165867187221025No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC3920.4665944556199636No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT2710.32256912620665845No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA2260.2690059871686525No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCC2020.24043897968171593No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAT1540.18330496470784283No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTATACAC1340.15949912513539571No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTA1280.15235737326366158No Hit
GCTTGGGATTTTCCCTGCCTCTAGTTTTGACCTGTTTTTCTAATCTTGCTTT1170.13926416149881565No Hit
TTCCTTGGGGTCTTCAGGTATGTTACCTTGACCTCCTACTGCACTCTTCTCC1140.13569328556294857No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGT1100.13093211764845916No Hit
ATATGTGGGGGATTTTTCTGTGTATCAGTGTAGCATGCCTGATCAGCTTTCT1080.12855153369121444No Hit
CACATCGGGCATCAGCAGCATTTGTCATAGTTGAACACTCCTTTCTCCTAGA1080.12855153369121444No Hit
TCACCAGGGACATCTGAAAAGTGGTCAATATCTAAAATATATATGGAATGCA1070.1273612417125921No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGT1010.12021948984085797No Hit
CACAATGGGCAGACCCAAGAATGTGAATATAGAAACTAGGAAAGGTGGGTTG990.11783890588361326No Hit
CGTCCGGGATGCTTTTCCTTTTTTAGTAACAGAGTATTACCTTTTTTAAAAT950.11307773796912382No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAACTCGTATGC880.10474569411876733No Hit
GCACCCGGCCACAGAGTTGGGTTTTTGAAAAGATCAAAACTAACAAACTCTT860.10236511016152262No Hit
ATCTGAGGGGAGAGGTCAGACTCTTCAAAGAATACACCATCATGAGAATTTT850.10117481818290026No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)