FastQCFastQC Report
Sat 31 Dec 2022
SRR5847409.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847409.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259930
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT43751.6831454622398336No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC34911.343053899126688No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC6310.24275766552533376No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC6260.24083407071134535No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT5250.20197745546878007No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA4460.17158465740776363No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGT3490.13426691801638901No Hit
ACATAAGGGAAGTTAGTCACTAATTATAATGAATATACAGAATAAAAGAAAC3190.12272534913245874No Hit
CCAACCGGGAAAATTGGAAGCGACACCTTAAAAAGAACTTTGTGGGAAGAAA3030.11656984572769591No Hit
CCCAGGGGGAATGCAGAAAGGAAATATTGCCAAAGAAATTAAAGAAGTCTTA2730.10502827684376562No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)