FastQCFastQC Report
Sat 31 Dec 2022
SRR5847407.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847407.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258943
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT42831.6540319684254834No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC32671.2616676256936854No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC6180.2386625628033969No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC5980.23093885526930635No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT5220.2015887666397624No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA3890.1502261115380605No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGT3720.14366096013408355No Hit
CCAACCGGGAAAATTGGAAGCGACACCTTAAAAAGAACTTTGTGGGAAGAAA3000.1158556130113577No Hit
AGATTGGGGATTCTGAAATCAGAAGAGGCTTAACCTTCTTGAAACTGCTTTT2940.11353850075113056No Hit
ACATAAGGGAAGTTAGTCACTAATTATAATGAATATACAGAATAAAAGAAAC2840.1096766469840853No Hit
CCCAGGGGGAATGCAGAAAGGAAATATTGCCAAAGAAATTAAAGAAGTCTTA2820.10890427623067625No Hit
GTAAACGGGATGCATCAAGTGATTTAATATAAAATACGAAATTTCAAATCAT2680.10349768095681289No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)