FastQCFastQC Report
Sat 31 Dec 2022
SRR5847406.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847406.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106702
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT37583.521958351296133No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC28542.6747389927086656No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC4680.43860471218908736No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC4320.40486588817454217No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT2990.28021967723191693No Hit
TAAGTTGGGAGAACTGTAGTTCTTGCTTTGAAGTTCTGTTTCTTTAATCTTT2770.259601507000806No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA2520.23617176810181625No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTATACAC2040.19118666941575604No Hit
GTATACGGGATTTGTCCCTTTACACAAAGTGTTTGGTGGTCCCTGATCCAGG1830.17150568874060468No Hit
ATTGATGGGCATTACTGGCTGTGTGACCTGGGACAAGTAGCTTAACCTCTCT1650.15463627673333208No Hit
CACCAGGGACTGGGCTGAGTAAACATAGGCTATACTTTGGGTGAAAGTAGAC1630.1527618976214129No Hit
AAGCTGGGGGATACGTGATAGTTGATAAGCTTTAGGCATATGTCAATCTGTG1590.14901313939757455No Hit
ACACACGGGGAAGTTCTTTTCATCTTCATAGAGTCAATTTACTATTTCCAAG1540.1443271916177766No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAACTCGTATGC1490.13964124383797868No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGT1460.1368296751700999No Hit
GATTCGGGGGATTCTCCCAGCCTTCACATCTACTTACTCGGATGCTCCATTG1450.1358924856141403No Hit
TGATCGGGATAGCTTTGTGAGCTAAGGAGGAGAAGGTGTATCATCACCACTT1440.1349552960581807No Hit
TGTTATGGGGAGGCTTGGGATATTCAGTAACTTGTCCTGGATTACACAAGTA1440.1349552960581807No Hit
GTTGCAGGGTCCTGGAATGCCCAGATAGAGCCTGCCGGGGAATCGCCTCCAC1410.13214372739030195No Hit
TTTGCAGGGCTAAAATTTTTATACATGCGATTTAATTGTTAAAGCTTATGAT1370.12839496916646362No Hit
CCGCGCGGGATTCCTAAAAGCTTTTGAATTTTAAAAACAAATGCCTAGAAAG1370.12839496916646362No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTA1360.12745777961050403No Hit
CTGTCTGGGGTTCTGCTGGATTTTGAGGAAGTATTTTCAAATCAATAGAACT1340.12558340049858485No Hit
TATTTAGGGAGGCCTCACCATTTCTATTGCAACCATTAAACGGTGACATTTT1320.12370902138666567No Hit
GCTGCTGGGTTAAACTCCTGGGCTCAAGCGATCCACCCACCTTGGGCTCACA1310.12277183183070609No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCC1270.11902307360686772No Hit
TGTTGCGGGAAGACTTCAAAAGCAAATGCAACAAAAATAAAAATAGAGAAAT1260.11808588405090813No Hit
TCACTAGGGAGATTAATTAGATGTATAGTAAAATATTGTTAAATGTCAGTTT1250.11714869449494854No Hit
CGTTCAGGGACCCTAACTGTAGCCTACATCTTTCTCCACGTTTATATAATTT1250.11714869449494854No Hit
ACTGTGGGGATTTTATGATTAACCAGTTAACTGAAACCTAAAAACCTGTATT1250.11714869449494854No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACCTCGTATGCCGTCT1240.11621150493898895No Hit
TATGCTGGGAGGTAAGAAGTAATTCTTCACAATTATTTCAAAACCATTTCTG1230.11527431538302937No Hit
AATCAAGGGGTAGGCATATGAAAGAAAAGGCACTTCTTGGACAAGCACTGAA1210.1133999362711102No Hit
AACTATGGGACCACTGGCAGGGCAATGATGGGAGGAAGAAGAATTCTTATCT1200.11246274671515061No Hit
ATAACAGGGATGTCTTGAATGATATTGCCTAGATTTTCTTCTAGGGTTTTTA1200.11246274671515061No Hit
GTATCTGGGGTACGTGCACAGGTGTGCTACACATGTGCACACATGCACAGTT1190.11152555715919102No Hit
GCTCATGGGCTATAGTGTGCTATATTCAAAGCACTTACAAAGGTATTTATTA1190.11152555715919102No Hit
TCATCAGGGCACCCAATTCCATAATATCAGTAGGGTCTTTTTGACTTCTGAG1190.11152555715919102No Hit
GGGTGGGGGAGAGCACATGGACAATTACCATCTCATGCAGGCATTTGGTTTT1190.11152555715919102No Hit
AACAGCGGGGCATCACAGTTTTCAATATACAAGTCTTTCACGAAACTCTTTA1180.11058836760323143No Hit
CATTCAGGGGTGTATTAGACCAGACTGCTTACTTAAAGTTTTATTGCGCTAA1180.11058836760323143No Hit
GTAAGTGGGAGATGAAAAATTCAAGAAAATAATCTCATTTCTAATAGCATCA1170.10965117804727184No Hit
CACTGAGGGATTCACCACAGCACAAAACTGACTTTCTCAGTATAAATTTCAT1170.10965117804727184No Hit
GCTTCAGGGACAGAATCTATTTTTTAAATTACAGCTAGACACTTTATACATG1170.10965117804727184No Hit
GTTAAAGGGATTTGCACTCTGGCTTTTTAAGATAGTAGTTGCCAAAATCCAG1170.10965117804727184No Hit
TAGTGGGGTGGGTTGCGTTTTTATTCTGTTGATACTATCTTCTGATGTATAA1160.10871398849131225No Hit
TGCACAGGGCACCAAAATAGAACCTCCTTAAGACACAAATCCAACAGAACCT1160.10871398849131225No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAT1160.10871398849131225No Hit
TTCTAAAGGGCTGGTAATATTTAGAAACATTTATTTTGAGATAAAGGAGAGC1150.10777679893535268No Hit
GCAGCCGGGAGTGGTTATCATGGTAAATTTCCTAGATTCTCCCTTATTTAAC1120.10496523026747391No Hit
TTAGCGGGGAGTCCGAAATAAGAATAATGATTTCAGACAGACATCAATATGT1110.10402804071151431No Hit
CTTGTGCCGGGGTCAGACACCATGCTAGATGCTGGGTATGCAGAAATAAGAG1100.10309085115555472No Hit
GACAATGGGATAATTTGAAGACATTTTCTTCCATTCTGTGGGTTGACTTTTC1090.10215366159959513No Hit
CACCAAGGGATTCAATTACAAGAATACATACTATAAAAAGGTTTTCTTCAAC1090.10215366159959513No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTC1070.10027928248767595No Hit
ATCGAGGGAGTTGCCAAAGACTGGAAGCAATCAAGATGTCTTCAGTGGGTGA1070.10027928248767595No Hit
GGCACAGGGCAAACCTAGAAAAAAGTCCAAAATGGTAAAATGACTTTAAGAT1070.10027928248767595No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)