FastQCFastQC Report
Sat 31 Dec 2022
SRR5847405.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847405.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104923
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT37153.5406917453751796No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC27362.6076265451807514No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC4610.4393698235849146No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC3550.33834335655671305No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT3520.3354841169238394No Hit
TAAGTTGGGAGAACTGTAGTTCTTGCTTTGAAGTTCTGTTTCTTTAATCTTT2920.2782993242663667No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA2560.24398844867188318No Hit
GTATACGGGATTTGTCCCTTTACACAAAGTGTTTGGTGGTCCCTGATCCAGG2050.19538137491303145No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTATACAC1820.17346053772766695No Hit
CACCAGGGACTGGGCTGAGTAAACATAGGCTATACTTTGGGTGAAAGTAGAC1690.1610704993185479No Hit
ATTGATGGGCATTACTGGCTGTGTGACCTGGGACAAGTAGCTTAACCTCTCT1580.1505866206646779No Hit
CACTGAGGGATTCACCACAGCACAAAACTGACTTTCTCAGTATAAATTTCAT1520.14486814139893064No Hit
AACAGCGGGGCATCACAGTTTTCAATATACAAGTCTTTCACGAAACTCTTTA1510.1439150615213061No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGT1430.13629042250030976No Hit
CCGCGCGGGATTCCTAAAAGCTTTTGAATTTTAAAAACAAATGCCTAGAAAG1410.13438426274506066No Hit
TTCTAAAGGGCTGGTAATATTTAGAAACATTTATTTTGAGATAAAGGAGAGC1380.13152502311218703No Hit
TGTTATGGGGAGGCTTGGGATATTCAGTAACTTGTCCTGGATTACACAAGTA1380.13152502311218703No Hit
GTATCTGGGGTACGTGCACAGGTGTGCTACACATGTGCACACATGCACAGTT1360.12961886335693795No Hit
GGGTGGGGGAGAGCACATGGACAATTACCATCTCATGCAGGCATTTGGTTTT1340.12771270360168885No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAACTCGTATGC1330.1267596237240643No Hit
TTTGCAGGGCTAAAATTTTTATACATGCGATTTAATTGTTAAAGCTTATGAT1320.12580654384643977No Hit
AACTATGGGACCACTGGCAGGGCAATGATGGGAGGAAGAAGAATTCTTATCT1310.12485346396881522No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAT1290.12294730421356613No Hit
GATTCGGGGGATTCTCCCAGCCTTCACATCTACTTACTCGGATGCTCCATTG1270.12104114445831707No Hit
GTTGCAGGGTCCTGGAATGCCCAGATAGAGCCTGCCGGGGAATCGCCTCCAC1250.11913498470306798No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCC1250.11913498470306798No Hit
TTAACCGGGACTGCCTCGCAATTTAAAATGACAAGCAAATGAGGATAGGAGA1210.1153226651925698No Hit
ACTGTGGGGATTTTATGATTAACCAGTTAACTGAAACCTAAAAACCTGTATT1210.1153226651925698No Hit
ATCGAGGGAGTTGCCAAAGACTGGAAGCAATCAAGATGTCTTCAGTGGGTGA1200.11436958531494525No Hit
TGTTGCGGGAAGACTTCAAAAGCAAATGCAACAAAAATAAAAATAGAGAAAT1200.11436958531494525No Hit
CATTCAGGGGTGTATTAGACCAGACTGCTTACTTAAAGTTTTATTGCGCTAA1190.11341650543732071No Hit
TCATCAGGGCACCCAATTCCATAATATCAGTAGGGTCTTTTTGACTTCTGAG1190.11341650543732071No Hit
GCTGCTGGGTTAAACTCCTGGGCTCAAGCGATCCACCCACCTTGGGCTCACA1190.11341650543732071No Hit
GTATATGGGGGAGAAGCCACCGGAAGCGGAAGCCAGAATGGCGCCATTTTGC1180.11246342555969616No Hit
TCACTAGGGAGATTAATTAGATGTATAGTAAAATATTGTTAAATGTCAGTTT1170.11151034568207162No Hit
AAGCTGGGGGATACGTGATAGTTGATAAGCTTTAGGCATATGTCAATCTGTG1160.11055726580444707No Hit
ACGGCTGGGATGGCTGCATAGTATTAGTATTCCATTATGGAATATAATGTTT1140.10865110604919798No Hit
GTCAATAGGGGGAAGCGGGCTGCGGGCCTGCGCTGGGCGCTACGGGACTGGG1140.10865110604919798No Hit
CGTTCAGGGACCCTAACTGTAGCCTACATCTTTCTCCACGTTTATATAATTT1120.10674494629394889No Hit
GCAGCCGGGAGTGGTTATCATGGTAAATTTCCTAGATTCTCCCTTATTTAAC1120.10674494629394889No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTC1110.10579186641632435No Hit
AGTTCCGGAATGTGATATTTGTGTAAAGACAGACATAAATCAATAGGTAAAA1110.10579186641632435No Hit
TGCACAGGGCACCAAAATAGAACCTCCTTAAGACACAAATCCAACAGAACCT1090.10388570666107527No Hit
TTTTAAGGGACTTTGTATATGACTTCCTTATTCCTGCATTCTATTTAATGTC1090.10388570666107527No Hit
TATCGGGGACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTCGGCGC1090.10388570666107527No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTA1090.10388570666107527No Hit
ACAGCAGGGACTGGCACCAAAAGAAATAGATCCAGATCTACTTGGTTGTCAA1080.10293262678345073No Hit
TTAGCGGGGAGTCCGAAATAAGAATAATGATTTCAGACAGACATCAATATGT1080.10293262678345073No Hit
GTAAGTGGGAGATGAAAAATTCAAGAAAATAATCTCATTTCTAATAGCATCA1070.10197954690582618No Hit
TAGTGGGGTGGGTTGCGTTTTTATTCTGTTGATACTATCTTCTGATGTATAA1070.10197954690582618No Hit
GGCACAGGGCAAACCTAGAAAAAAGTCCAAAATGGTAAAATGACTTTAAGAT1070.10197954690582618No Hit
TGATCGGGATAGCTTTGTGAGCTAAGGAGGAGAAGGTGTATCATCACCACTT1060.10102646702820164No Hit
GTTGCAGGGGCAGATCAAACAGCCCTGTATTGGAAGAAGATGCCCATCTAGG1060.10102646702820164No Hit
GGACATGGGAATCTGATAACACCATCAAAAACACATTACTTCTTTTGGCATA1050.10007338715057709No Hit
TGTATGGGGGAAGTTCCTGGAACACAGTACACTCTCAATAATTTGTTGTTAA1050.10007338715057709No Hit
CTCAGCTGGGACTACAGTAAATATTTTATCTAGAATTTAAAGCAAGTTTAGC1050.10007338715057709No Hit
ACACACGGGGAAGTTCTTTTCATCTTCATAGAGTCAATTTACTATTTCCAAG1050.10007338715057709No Hit
AACGCAGGGCGGCGAGATTTGATCTGTGGGCTACAGTTTGCCGACCCATGCA1050.10007338715057709No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)