FastQCFastQC Report
Sat 31 Dec 2022
SRR5847404.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847404.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104555
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT36413.4823776959495003No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC27142.59576299555258No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC4450.42561331356702214No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC4330.41413610061690015No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT2890.27640954521543687No Hit
TAAGTTGGGAGAACTGTAGTTCTTGCTTTGAAGTTCTGTTTCTTTAATCTTT2510.2400650375400507No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA2460.23528286547749988No Hit
GTATACGGGATTTGTCCCTTTACACAAAGTGTTTGGTGGTCCCTGATCCAGG2080.19893835780211375No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGT1760.16833245660178853No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAACTCGTATGC1760.16833245660178853No Hit
ATTGATGGGCATTACTGGCTGTGTGACCTGGGACAAGTAGCTTAACCTCTCT1640.1568552436516666No Hit
AACAGCGGGGCATCACAGTTTTCAATATACAAGTCTTTCACGAAACTCTTTA1610.1539859404141361No Hit
CACCAGGGACTGGGCTGAGTAAACATAGGCTATACTTTGGGTGAAAGTAGAC1590.1520730715891158No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTATACAC1560.1492037683515853No Hit
TGTTATGGGGAGGCTTGGGATATTCAGTAACTTGTCCTGGATTACACAAGTA1550.1482473339390751No Hit
TTTGCAGGGCTAAAATTTTTATACATGCGATTTAATTGTTAAAGCTTATGAT1460.13963942422648368No Hit
TCACTAGGGAGATTAATTAGATGTATAGTAAAATATTGTTAAATGTCAGTTT1460.13963942422648368No Hit
GTTGCAGGGTCCTGGAATGCCCAGATAGAGCCTGCCGGGGAATCGCCTCCAC1430.1367701209889532No Hit
TGATCGGGATAGCTTTGTGAGCTAAGGAGGAGAAGGTGTATCATCACCACTT1410.13485725216393288No Hit
GCAGCCGGGAGTGGTTATCATGGTAAATTTCCTAGATTCTCCCTTATTTAAC1370.13103151451389222No Hit
ACACACGGGGAAGTTCTTTTCATCTTCATAGAGTCAATTTACTATTTCCAAG1360.13007508010138205No Hit
ATCGAGGGAGTTGCCAAAGACTGGAAGCAATCAAGATGTCTTCAGTGGGTGA1340.12816221127636174No Hit
GTAAGTGGGAGATGAAAAATTCAAGAAAATAATCTCATTTCTAATAGCATCA1330.12720577686385154No Hit
GTATCTGGGGTACGTGCACAGGTGTGCTACACATGTGCACACATGCACAGTT1290.12338003921381091No Hit
CCGCGCGGGATTCCTAAAAGCTTTTGAATTTTAAAAACAAATGCCTAGAAAG1290.12338003921381091No Hit
TGTTGCGGGAAGACTTCAAAAGCAAATGCAACAAAAATAAAAATAGAGAAAT1280.12242360480130075No Hit
CTGTCTGGGGTTCTGCTGGATTTTGAGGAAGTATTTTCAAATCAATAGAACT1270.1214671703887906No Hit
GGGTGGGGGAGAGCACATGGACAATTACCATCTCATGCAGGCATTTGGTTTT1250.11955430156377025No Hit
CGTTCAGGGACCCTAACTGTAGCCTACATCTTTCTCCACGTTTATATAATTT1210.11572856391372961No Hit
ACTGTGGGGATTTTATGATTAACCAGTTAACTGAAACCTAAAAACCTGTATT1200.11477212950121946No Hit
TATTTAGGGAGGCCTCACCATTTCTATTGCAACCATTAAACGGTGACATTTT1190.1138156950887093No Hit
ATAACAGGGATGTCTTGAATGATATTGCCTAGATTTTCTTCTAGGGTTTTTA1180.11285926067619913No Hit
GCTCATGGGCTATAGTGTGCTATATTCAAAGCACTTACAAAGGTATTTATTA1180.11285926067619913No Hit
CACTGAGGGATTCACCACAGCACAAAACTGACTTTCTCAGTATAAATTTCAT1160.11094639185117881No Hit
CCAACAGGGCTTATCCCCACCTTGGCTATCATCACCCGATGAGGCAACCAGC1160.11094639185117881No Hit
TTCTAAAGGGCTGGTAATATTTAGAAACATTTATTTTGAGATAAAGGAGAGC1150.10998995743866866No Hit
CTATGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1140.10903352302615847No Hit
CTGCAAGGGGGAAAGGAAAGAATGGATATTGGGGGCAATTGGCAGTCTCTGA1130.10807708861364831No Hit
GATTCGGGGGATTCTCCCAGCCTTCACATCTACTTACTCGGATGCTCCATTG1130.10807708861364831No Hit
AACTATGGGACCACTGGCAGGGCAATGATGGGAGGAAGAAGAATTCTTATCT1120.10712065420113816No Hit
TATGCTGGGAGGTAAGAAGTAATTCTTCACAATTATTTCAAAACCATTTCTG1120.10712065420113816No Hit
AAGCTGGGGGATACGTGATAGTTGATAAGCTTTAGGCATATGTCAATCTGTG1120.10712065420113816No Hit
CACCAAGGGATTCAATTACAAGAATACATACTATAAAAAGGTTTTCTTCAAC1100.10520778537611783No Hit
GGCACAGGGCAAACCTAGAAAAAAGTCCAAAATGGTAAAATGACTTTAAGAT1100.10520778537611783No Hit
TAGTGGGGTGGGTTGCGTTTTTATTCTGTTGATACTATCTTCTGATGTATAA1090.10425135096360767No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCC1090.10425135096360767No Hit
GGACATGGGAATCTGATAACACCATCAAAAACACATTACTTCTTTTGGCATA1080.10329491655109752No Hit
CTCGCTGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATG1080.10329491655109752No Hit
GTATATGGGGGAGAAGCCACCGGAAGCGGAAGCCAGAATGGCGCCATTTTGC1070.10233848213858734No Hit
GTACAAGGAAGAAGCTTGTAGTTTATTGGGAAAGACTGAAGGATGAACTTAC1060.10138204772607719No Hit
ACAGCAGGGACTGGCACCAAAAGAAATAGATCCAGATCTACTTGGTTGTCAA1060.10138204772607719No Hit
TTGATAGGGAGGATCTTTCTATATGCATTTACAACAACAAAGTTAGCACTCA1060.10138204772607719No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTA1060.10138204772607719No Hit
CGTTCTGGGACATGAAGCAAAAGTTGTTAAAATTAAGTGGAGAAATTGATAA1050.10042561331356703No Hit
TTAACCGGGACTGCCTCGCAATTTAAAATGACAAGCAAATGAGGATAGGAGA1050.10042561331356703No Hit
CGGCTTGGGAGTTAATGGAAGAGCCTGAAGTAGAACTGGAATATTGAAGTTT1050.10042561331356703No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)