FastQCFastQC Report
Sat 31 Dec 2022
SRR5847401.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847401.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135400
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT48043.5480059084194977No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC38872.8707533234859675No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC5950.43943870014771047No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC5370.39660265878877404No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA4170.30797636632200887No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT3700.27326440177252587No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCC3480.2570162481536189No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAACTCGTATGC2400.1772525849335303No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGT1980.1462333825701625No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTATACAC1980.1462333825701625No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAT1700.12555391432791727No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTA1480.10930576070901034No Hit
ATGGCTGGGTCTCTCCCAAGCACACAGATTCTATCTCAGTGCTGTGCTGCCT1440.10635155096011817No Hit
CGCAAAGGGGGCAGATAACAAATTAAAAAACAAATGAGTCAGTTCTTGAAAA1380.1019202363367799No Hit
TAAATAGGGACAGTCAAGGGCATAACATTTTACGATTCAGTAAAAGCAATTC1370.10118168389955688No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)