FastQCFastQC Report
Sat 31 Dec 2022
SRR5847400.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847400.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103665
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT47914.6216177108956735No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC41203.974340423479477No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC7780.7504943809386002No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC5800.5594945256354604No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT5400.5209086962812907No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA5390.5199440505474364No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAT2410.2324796218588723No Hit
GCTCTTGGGGGGATTCTGCATGTAAATGAGAAGATGTGGCATTGGTCTTTCT2090.2016109583755366No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTATACAC2050.19775237544011962No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGT1980.19099985530313993No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAACTCGTATGC1950.1881059181015772No Hit
AATTGAGGGGCCCAAAGTCCAATCCAAGGTCACAGATGTTTCCATGTCTTCT1910.18424733516616024No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCC1870.18038875223074327No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTA1700.16398977475522114No Hit
ATCCACCCGGGGCAAGGTAGGTGTTTTTGAGAATGCTTGAGAGAAGGAAATT1690.1630251290213669No Hit
TATAGAGGGAACTAGTAATTATTATATTTTGTGGCAATATTGTGATTCGTGG1690.1630251290213669No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACCTCGTATGC1580.15241402594897024No Hit
TCTTCAGGGGCCTTTTCTCCGTCTGCCAATGGTGTTGAGGGAGAAGAGAGCC1570.151449380215116No Hit
GCATCTGGGAAGAGGTTTTATTTATAGCTAACTTTTGTTTTCAATAGGTAAT1550.1495200887474075No Hit
AGGCTGGGGTGTACCTAGTGTTGTCCCAAGTAGCAGAAGGTGAGGCAGCTTG1470.1418029228765736No Hit
CAGACCTGGGGGCCCTGTGGTTTATGACGTGCTGCTGGATAAGCATTTATGT1450.1398736314088651No Hit
TTTCATGGGGGACACCAAAAGTGAGCAGGAGTAGCTATTCTTATATCAGACA1430.1379443399411566No Hit
CGCTCTGGGGGGCCCCAACTGGTTCCGGGCTTTTCCGTCAGCCCGCCGGGGA1430.1379443399411566No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCTGTCTCTTATA1420.13697969420730238No Hit
CAATCGGGGGAGCCCTTGTAGAATGGTTTAGGGACGGGGTGATACAGTGATT1410.13601504847344814No Hit
TATTATGGGAGTATTATGCTGATACCAAAACTAGACAAAGACATGACAAGAA1350.13022717407032267No Hit
ATCAGTGGGAGGGTGGAATCTGCATATTCTGTCCTCAACCAAAAATAACTAG1340.12926252833646842No Hit
GCTTTGGGGAAGCCAAGTCAGATAACCTCTTGAACTATCTTTTTGTAGCCAT1340.12926252833646842No Hit
AATCTGGGGTGCTGTATAAACAAAATTAAATATGCAGTGCCTTTTCAATTAC1340.12926252833646842No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACCTCGTATGCCGTCT1340.12926252833646842No Hit
GCAACGGGGCATTCCATCATCAAAGGTATTGATAACAGAACATGGCGACTTG1330.12829788260261418No Hit
TTTCATGGGAATTCTGAATTTGCTTTTCTTGCCTCTCTTTAGTCACCTGTCA1300.12540394540105146No Hit
AATGCCGGGGCTTGACCCCAGGAGGTCGAGGCTGCAGTGAGCCGCGCTCATG1300.12540394540105146No Hit
CCATAAGGGATTTCAATCTCATTCTGGATATGTGTTCATTGAGTAAAGGAGG1290.12443929966719722No Hit
TCAAGTCGGGGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATA1270.12251000819948875No Hit
ACTTATGGGAATGACTGAGTGAACAGTTCCTATCTGTATATTTGACTAAACC1240.11961607099792602No Hit
CGAAACGGGAGAACCTGAAGGCAGAAGAAAAATCTTAAAATCAAAAAGAAAC1210.1167221337963633No Hit
AAAATGGGGGAAATATTAGTTCATGGATGGTGGTCATTTATTAAGACGTAAA1190.1147928423286548No Hit
CTTAAGGGGGGGTCGCGGCGATCCTCCCACATGTCATCAGCAGGATGGGCAA1190.1147928423286548No Hit
GTTTGAGGGATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCG1160.11189890512709208No Hit
TTTGACGGGGTGGTGGAGGGTGCCTGTAATCTCGGCCTCCCCAGTGGCTGGG1160.11189890512709208No Hit
TAGCAGGGGATAGTCAACTCGGTTATTCAAGTGCCACGTATTCAGTTTATAG1160.11189890512709208No Hit
TCTGCGGGGACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGC1140.1099696136593836No Hit
TCGACAGGGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCA1130.10900496792552936No Hit
ATGACAGGGATAGCAAAACTGAATGTCAAAAGTACCTTCTGTCCACACACAC1130.10900496792552936No Hit
AACGTAGGGGGTCCAAATCAGACCACTTTCTCCTGGGATACTGCTGCTTTTA1130.10900496792552936No Hit
TATTTAGGGATGGTCCAGACCTTGTGACTGAGCAGATTGGGAGTTGGAATGA1120.1080403221916751No Hit
TGCCGCCGGGTGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTC1110.10707567645782086No Hit
AGATATGGGGGGTCTCTGAAATGCTTGATTGAACTTTAATTTTCATATACAA1090.10514638499011238No Hit
CACTAGGGGTGCTCTAGGGAAGGAAGCCAGGAAAGAGAATCCTCCCCACTCA1090.10514638499011238No Hit
TATACAGGGAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCT1080.10418173925625814No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTC1070.10321709352240391No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTC1070.10321709352240391No Hit
AAATACGGGACATCATCGAGGTAATGGACAGCTCAATGCCGCTGATTGGAGA1060.10225244778854965No Hit
ACTGCCGGGTGTCTCATGTCATGTGAAAGACAGCATACCTATATTTGTGTAA1060.10225244778854965No Hit
AATTGAGGGTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTG1050.10128780205469541No Hit
GCGACGGGATAAAAAAGAATGAGATACTGTCATTTGCAACAACAATGATGGA1040.10032315632084118No Hit
ACGTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1040.10032315632084118No Hit
GCACCCCGGCCCGGTGCCTCACACCCCGCTACCCCATGCATCCAGACTCTAA1040.10032315632084118No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)