FastQCFastQC Report
Sat 31 Dec 2022
SRR5847399.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847399.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102141
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT48944.7914157879793615No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC40733.987624949824262No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC7040.6892433009271498No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC5940.5815490351572826No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT4820.4718966918279633No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA4680.45819014891179843No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAT2430.23790642347343377No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTATACAC2090.2046191049627476No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGT2030.1987448722843912No Hit
GCTCTTGGGGGGATTCTGCATGTAAATGAGAAGATGTGGCATTGGTCTTTCT1990.19482871716548694No Hit
AATTGAGGGGCCCAAAGTCCAATCCAAGGTCACAGATGTTTCCATGTCTTCT1970.1928706396060348No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAACTCGTATGC1900.18601736814795233No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCC1900.18601736814795233No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTA1860.18210121302904808No Hit
TCTTCAGGGGCCTTTTCTCCGTCTGCCAATGGTGTTGAGGGAGAAGAGAGCC1840.18014313546959596No Hit
GCTTTGGGGAAGCCAAGTCAGATAACCTCTTGAACTATCTTTTTGTAGCCAT1780.17426890279123958No Hit
CAATCGGGGGAGCCCTTGTAGAATGGTTTAGGGACGGGGTGATACAGTGATT1640.16056235987507464No Hit
CAGACCTGGGGGCCCTGTGGTTTATGACGTGCTGCTGGATAAGCATTTATGT1600.1566462047561704No Hit
CGCTCTGGGGGGCCCCAACTGGTTCCGGGCTTTTCCGTCAGCCCGCCGGGGA1580.15468812719671826No Hit
AGGCTGGGGTGTACCTAGTGTTGTCCCAAGTAGCAGAAGGTGAGGCAGCTTG1570.1537090884169922No Hit
GCAACGGGGCATTCCATCATCAAAGGTATTGATAACAGAACATGGCGACTTG1550.1517510108575401No Hit
AATCTGGGGTGCTGTATAAACAAAATTAAATATGCAGTGCCTTTTCAATTAC1510.1478348557386358No Hit
GCATCTGGGAAGAGGTTTTATTTATAGCTAACTTTTGTTTTCAATAGGTAAT1510.1478348557386358No Hit
TATACAGGGAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCT1470.14391870061973155No Hit
TATAGAGGGAACTAGTAATTATTATATTTTGTGGCAATATTGTGATTCGTGG1440.14098158428055335No Hit
ATCCACCCGGGGCAAGGTAGGTGTTTTTGAGAATGCTTGAGAGAAGGAAATT1430.14000254550082727No Hit
CGAAACGGGAGAACCTGAAGGCAGAAGAAAAATCTTAAAATCAAAAAGAAAC1330.13021215770356664No Hit
ACTGCCGGGTGTCTCATGTCATGTGAAAGACAGCATACCTATATTTGTGTAA1320.1292331189238406No Hit
AATGCCGGGGCTTGACCCCAGGAGGTCGAGGCTGCAGTGAGCCGCGCTCATG1320.1292331189238406No Hit
AGATATGGGGGGTCTCTGAAATGCTTGATTGAACTTTAATTTTCATATACAA1310.1282540801441145No Hit
ATGACAGGGATAGCAAAACTGAATGTCAAAAGTACCTTCTGTCCACACACAC1270.12433792502521024No Hit
CTCCGCGGACAACAGTGCCACCCAATCTTCGAAAACTGGATCCAGAACTCCA1270.12433792502521024No Hit
GATTCAGGGGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAA1260.12335888624548418No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCTGTCTCTTATA1230.12042176990630599No Hit
ACTTATGGGAATGACTGAGTGAACAGTTCCTATCTGTATATTTGACTAAACC1200.1174846535671278No Hit
TTTCATGGGGGACACCAAAAGTGAGCAGGAGTAGCTATTCTTATATCAGACA1190.11650561478740173No Hit
TTTGACGGGGTGGTGGAGGGTGCCTGTAATCTCGGCCTCCCCAGTGGCTGGG1190.11650561478740173No Hit
CCATAAGGGATTTCAATCTCATTCTGGATATGTGTTCATTGAGTAAAGGAGG1190.11650561478740173No Hit
ATCAGTGGGAGGGTGGAATCTGCATATTCTGTCCTCAACCAAAAATAACTAG1180.11552657600767567No Hit
CTTGGCGGGATTTGAAAGCACTTAAGCACTCCTCCTTGTGGAAAGAATATAC1170.11454753722794961No Hit
AACGTAGGGGGTCCAAATCAGACCACTTTCTCCTGGGATACTGCTGCTTTTA1150.11258945966849748No Hit
TTTCATGGGAATTCTGAATTTGCTTTTCTTGCCTCTCTTTAGTCACCTGTCA1150.11258945966849748No Hit
TATTATGGGAGTATTATGCTGATACCAAAACTAGACAAAGACATGACAAGAA1150.11258945966849748No Hit
GTCGGGGGGACACTTCGAATATTCTACTAAATGAGAGTTCTGAGTATAGAAA1140.1116104208887714No Hit
AATTCTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1130.11063138210904534No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATG1130.11063138210904534No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTC1100.10769426576986714No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCTGTCTC1100.10769426576986714No Hit
TCGACAGGGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCA1100.10769426576986714No Hit
ACACTGGGGGGTGGAGAGAGCGCGGAGACAAGCGCAGAGCGCAGCGCACGGC1090.10671522699014108No Hit
TCAAGTCGGGGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATA1090.10671522699014108No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTAT1090.10671522699014108No Hit
CTTAAGGGGGGGTCGCGGCGATCCTCCCACATGTCATCAGCAGGATGGGCAA1080.10573618821041501No Hit
GAAAAAGGGATTCTCTGGAGCTATATATAAATCTTTGTTATGATCATGTCAT1070.10475714943068895No Hit
GCTGTAGGGGAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATA1050.10279907187123681No Hit
AATTGAGGGTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTG1050.10279907187123681No Hit
AGCTATGGGTCTTTGCAAAAATCCAAATATTGTTAAGGGACCAGAGAGATGC1050.10279907187123681No Hit
AGCCTGGGGGGAATTGGTATATTTCTTCCATTTCATTTTTCTATTTCTTTGC1040.10182003309151075No Hit
TGCCGCCGGGTGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTC1040.10182003309151075No Hit
GTTAGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCC1030.10084099431178468No Hit
GCGACGGGATAAAAAAGAATGAGATACTGTCATTTGCAACAACAATGATGGA1030.10084099431178468No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACCTCGTATGC1030.10084099431178468No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)