FastQCFastQC Report
Sat 31 Dec 2022
SRR5847398.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847398.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101548
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCCGTCT47254.6529719935400005No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGC39673.906526962618663No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTC7550.7434907629889314No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCTTATAC5930.5839602946389885No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCA4790.4716981132075472No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCCTGTCTCT3970.39094812305510696No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAT2370.23338716666010162No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCAACTCGTATGC2210.2176310710206011No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGT2120.20876826722338201No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATGCC2120.20876826722338201No Hit
AATTGAGGGGCCCAAAGTCCAATCCAAGGTCACAGATGTTTCCATGTCTTCT2120.20876826722338201No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTATACAC1900.18710363571906882No Hit
GCTCTTGGGGGGATTCTGCATGTAAATGAGAAGATGTGGCATTGGTCTTTCT1840.18119509985425614No Hit
AATCTGGGGTGCTGTATAAACAAAATTAAATATGCAGTGCCTTTTCAATTAC1760.17331705203450584No Hit
CAATCGGGGGAGCCCTTGTAGAATGGTTTAGGGACGGGGTGATACAGTGATT1710.16839327214716193No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACTGTCTCTTA1700.16740851616969316No Hit
TCTTCAGGGGCCTTTTCTCCGTCTGCCAATGGTGTTGAGGGAGAAGAGAGCC1670.1644542482372868No Hit
TTTCATGGGGGACACCAAAAGTGAGCAGGAGTAGCTATTCTTATATCAGACA1590.15657620041753653No Hit
TATAGAGGGAACTAGTAATTATTATATTTTGTGGCAATATTGTGATTCGTGG1550.1526371765076614No Hit
CAGACCTGGGGGCCCTGTGGTTTATGACGTGCTGCTGGATAAGCATTTATGT1500.1477133966203175No Hit
ATCCACCCGGGGCAAGGTAGGTGTTTTTGAGAATGCTTGAGAGAAGGAAATT1500.1477133966203175No Hit
GCATCTGGGAAGAGGTTTTATTTATAGCTAACTTTTGTTTTCAATAGGTAAT1490.14672864064284868No Hit
CGCTCTGGGGGGCCCCAACTGGTTCCGGGCTTTTCCGTCAGCCCGCCGGGGA1490.14672864064284868No Hit
GCTTTGGGGAAGCCAAGTCAGATAACCTCTTGAACTATCTTTTTGTAGCCAT1440.14180486075550477No Hit
TATACAGGGAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCT1370.13491156891322328No Hit
CGAAACGGGAGAACCTGAAGGCAGAAGAAAAATCTTAAAATCAAAAAGAAAC1310.1290030330484106No Hit
TCGACAGGGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCA1270.12506400913853546No Hit
TTTGACGGGGTGGTGGAGGGTGCCTGTAATCTCGGCCTCCCCAGTGGCTGGG1270.12506400913853546No Hit
CTTAAGGGGGGGTCGCGGCGATCCTCCCACATGTCATCAGCAGGATGGGCAA1260.12407925316106669No Hit
AATGCCGGGGCTTGACCCCAGGAGGTCGAGGCTGCAGTGAGCCGCGCTCATG1250.1230944971835979No Hit
ATCAGTGGGAGGGTGGAATCTGCATATTCTGTCCTCAACCAAAAATAACTAG1220.12014022925119155No Hit
CTTGGCGGGATTTGAAAGCACTTAAGCACTCCTCCTTGTGGAAAGAATATAC1210.11915547327372278No Hit
CTCCGCGGACAACAGTGCCACCCAATCTTCGAAAACTGGATCCAGAACTCCA1210.11915547327372278No Hit
AATTGAGGGTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTG1210.11915547327372278No Hit
ACTTATGGGAATGACTGAGTGAACAGTTCCTATCTGTATATTTGACTAAACC1210.11915547327372278No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTC1210.11915547327372278No Hit
GAAAAAGGGATTCTCTGGAGCTATATATAAATCTTTGTTATGATCATGTCAT1210.11915547327372278No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCTGTCTCTTATA1200.11817071729625399No Hit
GCAACGGGGCATTCCATCATCAAAGGTATTGATAACAGAACATGGCGACTTG1180.11620120534131641No Hit
TTTCATGGGAATTCTGAATTTGCTTTTCTTGCCTCTCTTTAGTCACCTGTCA1160.11423169338637885No Hit
TATTATGGGAGTATTATGCTGATACCAAAACTAGACAAAGACATGACAAGAA1160.11423169338637885No Hit
GCTATGGGGGACGACTAAAATTCTAATACTTTTAGTGTGCATGCGTGTATTT1150.11324693740891008No Hit
GCGACGGGATAAAAAAGAATGAGATACTGTCATTTGCAACAACAATGATGGA1130.1112774254539725No Hit
TCTGCGGGGACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGC1130.1112774254539725No Hit
ATGACAGGGATAGCAAAACTGAATGTCAAAAGTACCTTCTGTCCACACACAC1120.11029266947650371No Hit
TCAAGTCGGGGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATA1120.11029266947650371No Hit
AACGTAGGGGGTCCAAATCAGACCACTTTCTCCTGGGATACTGCTGCTTTTA1110.10930791349903494No Hit
GAATTTGGGCAAAATAAGTCTGGGAAAGAATGGCAAAAGGATCTTTCTATGG1090.10733840154409738No Hit
ACAGCTGGGGAAACAGCTTTACTACTTTTTTTTATTGCGTCTGGCCTCACTG1080.10635364556662859No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCATCTCGTATG1080.10635364556662859No Hit
CACTAGGGGTGCTCTAGGGAAGGAAGCCAGGAAAGAGAATCCTCCCCACTCA1070.1053688895891598No Hit
CCGATGGGGGTGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1060.10438413361169101No Hit
AGATATGGGGGGTCTCTGAAATGCTTGATTGAACTTTAATTTTCATATACAA1060.10438413361169101No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCACCTTTCACCTCGTATGC1060.10438413361169101No Hit
AGGCTGGGGTGTACCTAGTGTTGTCCCAAGTAGCAGAAGGTGAGGCAGCTTG1060.10438413361169101No Hit
GTCGGGGGGACACTTCGAATATTCTACTAAATGAGAGTTCTGAGTATAGAAA1050.10339937763422224No Hit
CCATAAGGGATTTCAATCTCATTCTGGATATGTGTTCATTGAGTAAAGGAGG1050.10339937763422224No Hit
ACTGCCGGGTGTCTCATGTCATGTGAAAGACAGCATACCTATATTTGTGTAA1050.10339937763422224No Hit
GTTAGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCC1040.10241462165675345No Hit
CGTGGCGGGAATATCCTTGGAGCTAGGATTTCAATCCAAGCAGCTTGGCATG1040.10241462165675345No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCTTTCAACTCGTATGCCGTCT1040.10241462165675345No Hit
TACAGCGGGGTAAGTTTGTGGTTTGTTTCTGGTTTACAGTGGTGGCTGACAC1030.10142986567928468No Hit
GATTCAGGGGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAA1020.10044510970181589No Hit
ATATAAGGGAAGGTATATCTGTAATTTAAAGATTACTGCAAATATCTTTACT1020.10044510970181589No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)