FastQCFastQC Report
Thu 19 Jan 2023
SRR5847396.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847396.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198843
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAAGAATCTCGTATGCCGTCT6550.32940561146230946No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCAAGAATCTCGTATGC4020.20216955085167695No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGG206.0852815E-446.3228511
CGTGGCA206.0852815E-446.3228513
GTTAGCA206.0852815E-446.3228515
CCGTCGC206.0852815E-446.3228511
TCGGCCC206.0852815E-446.3228514
ATCCGGT253.2689037E-546.3228512
CGCTCGC206.0852815E-446.3228515
GCTTCGA301.7663515E-646.3228516
GAGCGCC206.0852815E-446.3228514
TCGCTCG206.0852815E-446.3228514
CATGTCA206.0852815E-446.3228516
GTATACT206.0852815E-446.3228511
CGTCGCT206.0852815E-446.3228512
CACACGA206.0852815E-446.3228512
ATCATCG206.0852815E-446.3228510
TAATGCG206.0852815E-446.3228516
GCCGTCG206.0852815E-446.3228510
ATCGATT301.7663515E-646.3228510
TCCGGTT253.2689037E-546.3228513
CGTATAG206.3091615E-445.9846421
Produced by FastQC (version 0.11.5)