FastQCFastQC Report
Sat 31 Dec 2022
SRR5847394.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847394.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132437
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAAGAATCTCGTATGCCGTCT8830.6667321065865279No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCAAGAATCTCGTATGC6140.4636166630171327No Hit
TTTCTAGGGAATTTCCCAACTTTTGTCTTCAAATTAAAAAAGAAAATAGCAT1990.15026012368144853No Hit
GCTACAGGGGAGGACACGGGTCGCTGGCGGGTTAGGTGGCTGCGTGGCCGCC1940.14648474368945233No Hit
CGTTCAGGGGAGTCGCTATCGGAGGCCGCGCGGGTGGCTGAGCAGCGGTCTG1900.14346443969585537No Hit
GTAATAGGGAGGAGGGAGAGATTGGGAGGAGGGAAGAAAGTGAGGAATAGGC1830.1381789077070607No Hit
TTTACAGGGGGCTCACCATTATCAATCATCAGAAAAAGGTGAATCAAAACCA1800.135913679711863No Hit
TCAAGCGGGGGAATTAATGGCAGATTAAACATTGCTGAAGAAAAAATATTTG1790.13515860371346378No Hit
GCTTAGGGGTAGTTCATGCCTGTAGCTCTCTTCCTTTTGTTTCTCACCCTTC1740.13138322372146757No Hit
GTTCATGGGAGAGGCAGAGGCAGCATCCAGCGGCGGCGCCAGCAGTTCCAGT1630.12307738773907594No Hit
ACCGGGGAGTTTGTTACACGAGGTCTGAGAGACAGAGGCAGCGTGTTTGAGC1630.12307738773907594No Hit
TTCGCCGGGGACAAGAGCTTGATGAATGAAAAGAAGGAAGCCTGTGACAATT1630.12307738773907594No Hit
GATGACGGGCTGGAAGATAGCAGCAGAGTTTTTGATATCTTTGAACCCACTC1610.12156723574227746No Hit
AAGTACGGGCTGCAAGTGCTTCTCTGACATCCTTAGACTAAGTTGAGTACAC1590.12005708374547898No Hit
TGTACTGGGATTTTTAGTTGTCTTTATTTAAAAAATGAAACACATTAGTTAC1580.11930200774707975No Hit
TAATGTGGGAGTGCATGGTTATTTTACATAGTAAGACCTGGAATTTTATGGG1560.11779185575028125No Hit
GCATTTGGGAGTTACGGCAAACCTGAGATTTCAGCATAAAATCTTTAGTATG1550.11703677975188204No Hit
CAATTAGGGGGAGAGAAGGCAGGAAGAAGGCAAGGGACGAGACAACCATGCT1540.11628170375348279No Hit
TTTTATGGGATAACTTTGAAAAAAACATAATCTGTTAGATCGGTGAAGGATT1540.11628170375348279No Hit
TAAGCGCGGGGGGAGAAAGATGGCGGCAGCCGTGCGACAGGATTTGGCCCAG1530.11552662775508354No Hit
CGTTCCGGGATACAAAATGAGCAATCATAAGGATTTGAGAATCTGTTAAAGA1530.11552662775508354No Hit
TGTAATGGGACAATACTATGAAATAGGTATTATTATTGCCATTTTCCTGTGC1520.1147715517566843No Hit
AGTGTAGGGAATGAGTTTGTGCTCCAAACAAATGACACCACTGAGCAGCAGG1520.1147715517566843No Hit
TTCCGTGGGCAAGCAGCCGCCTCTAACAAACACCATGGTCCGTGGAAGTTCA1520.1147715517566843No Hit
CTAAGTGTGGGGATCTGTGGTTGGGGAGGCTGAGCTGGAAAATCTTGAAAAT1510.11401647575828508No Hit
GCGTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA1510.11401647575828508No Hit
AGTCAGGGAGAGTTTTTAGCATGAAGCGTTGTTGAATTTTGTCAAAGGCCTT1510.11401647575828508No Hit
TCACTGGGAGGGCTGACAGCAAGCTGTTGGCATTATTCACATTTGGGTCCTG1490.11250632376148659No Hit
TTGTAAGGGATGGCTGCAACAGGTGAGATGAGAGGGTTGGTTATAAAGTAGC1490.11250632376148659No Hit
TACCAAGGGGATCTTTAGCCGGGGTTTGAAAAGATTTTAAGGGGTCACGGAC1470.11099617176468812No Hit
ACTTCCGGGATCTGATTGTGGTTTTTGATTTGCATTTCTCAGATGAGAGTGA1470.11099617176468812No Hit
CGAAACGGGATTTGAGGTGGCTCTGATTTCCTGGCAGCTGAACTCGGGTAGT1460.11024109576628888No Hit
TCCGTCGGGAGTGGACATTCTGAGCAAAAGACCACATTTCTGTCCAATTTTG1460.11024109576628888No Hit
AAACCCGGATTGGATAAAGAGTCGAGACCCATCAGTGTGCTATATTCAGGAG1450.10948601976788963No Hit
TTGACAGGGAGTATGGGATACTGAAACAAGAGAAATTTATTTACTCATGGTT1450.10948601976788963No Hit
TAATACGGGTGCTCGGCAAAATAAACTATCAATAAACAAACAACCTATACAA1450.10948601976788963No Hit
GTGTCTGGGAATGTATTTACGTAACTCAAAATGATCAGACATGTCACAAGAA1440.1087309437694904No Hit
CAACAAGGGACAATTTCCTTTTTTCCATTCACCTGTCTTGATGTGCAGAAAA1440.1087309437694904No Hit
CATCAGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA1440.1087309437694904No Hit
ACTATGGGGGAGCTCTCAACTCCAAGTTGTAGATTTGGTGTCTTCCTTGTTC1440.1087309437694904No Hit
TGCAAAGGGGAAGTTAACCTGAAATATGTCCACTGGTTACTATAGCCAACAG1440.1087309437694904No Hit
TGTCTGGGGAAGTCTTATTGACTTTTAAGAAAGTAAAGATGATAAAAGGTGA1430.10797586777109115No Hit
CTTCGGGGGAGTCCAGAAATGTGATGATAGTAGCTTGAGAAGAAAATGCATC1430.10797586777109115No Hit
CACTAAGGGGAAAAGATGTAGGAATCTTGAAAAGTGTTCTGACACACTATGC1430.10797586777109115No Hit
GAACCTGGGACATGTGCATAGACGTTCCTGGGCTATGGAAGATAAAAAATCA1420.10722079177269192No Hit
AACTTTGGGGGGATGGGAGCCTTCTCCGGATCTTTTGTTCTTCTGCACCTCT1420.10722079177269192No Hit
CGGCGCGGGCGATCTTAGATAGGGATGAGCATTTCCCTTATGTGTCATATCT1410.10646571577429269No Hit
GCTTATGGGACCTCTAAAGTGTTTCCAAGTAGAGTCTGGCAGTATTAGTTTC1410.10646571577429269No Hit
GAGTTAGGGGCCCCCACCGGACTTGGTCGCGCCCGAAGTGTAACACTTTCTC1410.10646571577429269No Hit
TACTGCGGGGACTCGAGCCAGCTCAAGCCCGCAGCTCGCAGGGAGATCCAGC1400.10571063977589344No Hit
TTGGAGTGGGACCTGGACATATTTTATAAATGGGGAAACTGAAATTTAGACA1400.10571063977589344No Hit
TCATCTGGGAACTTACAGTGTTTTCTTCTCCAGGCAAAATTAATTTCCTAAC1390.1049555637774942No Hit
TATTGTGGGTTCTTGTCTTACCTGCGTAAGTTGAACCCAAAGTTCATAAGAA1380.10420048777909498No Hit
TGCATACGGGAGGTTTTAATAAATATCATTTCAGTTTTACAAGATGAAGAGA1380.10420048777909498No Hit
TCTTGCGGGATAGGAAATTCCTGTACCTTCTTGGCCCCCATAATGTGTTTTT1370.10344541178069573No Hit
GTGACCGGGATTCCTTACACAAAAAGAAAATACATATGCAGTCTAGTCAGAC1370.10344541178069573No Hit
AACGCAGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG1360.10269033578229649No Hit
AGACAGGGGATTTACATATGCTACAAACATATTTATTTACTGTGGGTTCGTT1360.10269033578229649No Hit
CGCCTGGGCAAGGAACTACACTCAGATGAAACCTCTGTGCTTATGTCTAGTG1360.10269033578229649No Hit
TCTTAGGGGCGCTCGCCCCCTTCAGGCCAGGTCCGGGTGGCCAGGGGTACCC1360.10269033578229649No Hit
GTAGTCGGGATTCTTTCAGTATATCATCACTGAAGACAGGAAGATAGGCACA1360.10269033578229649No Hit
TTCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1350.10193525978389724No Hit
TCGATAGGGGGGCCATTTCTTCCACATAAAACATGCATTTCTTGTAAAGATC1350.10193525978389724No Hit
AGACGGGGATTGTTTCACATCCTTACACCCTTATAACAAGCTCCTCTCCCTC1350.10193525978389724No Hit
AGTTGGGGATTGTAATAGTTTAGATGAGAAATGATGAAGATTTATACTAAAG1350.10193525978389724No Hit
GTACAGGGGATCTGGTACCGGGGTTGCCAACTTTTTAAATTAATTTTCATTA1340.10118018378549802No Hit
ATCACGGGATAGTTTACTATCAACCATAAAATGCATTTTATAATTTTGTAAT1340.10118018378549802No Hit
TAATTCGGGAACCTGTCTCTCCCTACACCCTCATTCTCAGACACCACTGATC1340.10118018378549802No Hit
TGACTAGGGATATAGAATCCATCTGCAAATTAGGTTCCCCCCACTAGATGCA1340.10118018378549802No Hit
TGCTCTGGGACTTTTACCTCTGTAGGAAATGTCTGATGGGAATGGTGCCATG1340.10118018378549802No Hit
ACCTGGGGGAAGTGATGCCTAAAGCTGTGGACCGCGTGGGCTCGCCTCCCTG1330.10042510778709877No Hit
CTAAACGGGGAGTAGGACGTTTTTAACAGTGTTTCAGAGAATGGCACTTGCT1330.10042510778709877No Hit
TGCAGTGGGAACACACCAAGGAATAAAACCATATAAATGTTCATGGTGTGGG1330.10042510778709877No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)