FastQCFastQC Report
Sat 31 Dec 2022
SRR5847393.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847393.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131330
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAAGAATCTCGTATGCCGTCT8230.6266656514124724No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCAAGAATCTCGTATGC6110.4652402345237189No Hit
TTTCTAGGGAATTTCCCAACTTTTGTCTTCAAATTAAAAAAGAAAATAGCAT2080.15837965430594683No Hit
TTTACAGGGGGCTCACCATTATCAATCATCAGAAAAAGGTGAATCAAAACCA1920.14619660397472017No Hit
ACCGGGGAGTTTGTTACACGAGGTCTGAGAGACAGAGGCAGCGTGTTTGAGC1900.14467372268331682No Hit
TAATACGGGTGCTCGGCAAAATAAACTATCAATAAACAAACAACCTATACAA1880.1431508413919135No Hit
GCTTAGGGGTAGTTCATGCCTGTAGCTCTCTTCCTTTTGTTTCTCACCCTTC1760.1340135536434935No Hit
CAATTAGGGGGAGAGAAGGCAGGAAGAAGGCAAGGGACGAGACAACCATGCT1750.13325211299779183No Hit
TAAGCGCGGGGGGAGAAAGATGGCGGCAGCCGTGCGACAGGATTTGGCCCAG1710.13020635041498516No Hit
AGAAAGGGGACACTGCATACACTTGACTAATTTCAACTGCCTATTGAAATCT1710.13020635041498516No Hit
TCACTGGGAGGGCTGACAGCAAGCTGTTGGCATTATTCACATTTGGGTCCTG1680.12792202847788015No Hit
TACCAAGGGGATCTTTAGCCGGGGTTTGAAAAGATTTTAAGGGGTCACGGAC1670.12716058783217848No Hit
GTAATAGGGAGGAGGGAGAGATTGGGAGGAGGGAAGAAAGTGAGGAATAGGC1670.12716058783217848No Hit
TTCGCCGGGGACAAGAGCTTGATGAATGAAAAGAAGGAAGCCTGTGACAATT1630.12411482524937181No Hit
GAGTTAGGGGCCCCCACCGGACTTGGTCGCGCCCGAAGTGTAACACTTTCTC1630.12411482524937181No Hit
GCTACAGGGGAGGACACGGGTCGCTGGCGGGTTAGGTGGCTGCGTGGCCGCC1620.12335338460367014No Hit
CGTTCAGGGGAGTCGCTATCGGAGGCCGCGCGGGTGGCTGAGCAGCGGTCTG1610.12259194395796848No Hit
ACCTGGGGGAAGTGATGCCTAAAGCTGTGGACCGCGTGGGCTCGCCTCCCTG1590.12106906266656514No Hit
GTAGTCGGGATTCTTTCAGTATATCATCACTGAAGACAGGAAGATAGGCACA1590.12106906266656514No Hit
TCAAGCGGGGGAATTAATGGCAGATTAAACATTGCTGAAGAAAAAATATTTG1570.1195461813751618No Hit
GGTACTGGGAGCTGGTGTGATTGGAGCTCAGAAGCCACAATATGATATCTGG1560.11878474072946013No Hit
TCTTGCGGGATAGGAAATTCCTGTACCTTCTTGGCCCCCATAATGTGTTTTT1550.11802330008375847No Hit
CACTAAGGGGAAAAGATGTAGGAATCTTGAAAAGTGTTCTGACACACTATGC1550.11802330008375847No Hit
GCATTTGGGAGTTACGGCAAACCTGAGATTTCAGCATAAAATCTTTAGTATG1540.11726185943805681No Hit
CGTTCCGGGATACAAAATGAGCAATCATAAGGATTTGAGAATCTGTTAAAGA1530.11650041879235513No Hit
ACTTCCGGGATCTGATTGTGGTTTTTGATTTGCATTTCTCAGATGAGAGTGA1520.11573897814665347No Hit
CAACTAAGGGGCAGCTGCCGGCAGGACCTTTCTCTCGCTGCCGCTGGGACCC1500.11421609685525014No Hit
GTTCATGGGAGAGGCAGAGGCAGCATCCAGCGGCGGCGCCAGCAGTTCCAGT1500.11421609685525014No Hit
AGTGTAGGGAATGAGTTTGTGCTCCAAACAAATGACACCACTGAGCAGCAGG1500.11421609685525014No Hit
TAGTATGGGCGATCTTTTGGCGGGGGGAGGGGCCAAGAGAAGGAAAACCCTG1500.11421609685525014No Hit
GCTAATGGGACTTGACACTTTTACTGCAGCTTCTAGTCTGTTAAGTGGTTTA1490.11345465620954846No Hit
TCGATAGGGGGGCCATTTCTTCCACATAAAACATGCATTTCTTGTAAAGATC1490.11345465620954846No Hit
AGACGGGGATTGTTTCACATCCTTACACCCTTATAACAAGCTCCTCTCCCTC1490.11345465620954846No Hit
CGCCTGGGCAAGGAACTACACTCAGATGAAACCTCTGTGCTTATGTCTAGTG1480.1126932155638468No Hit
AAACTTGGGGTGACTTTCTGCATTTTACTCAACGTTAGGATTTCAGCAGTGA1480.1126932155638468No Hit
TTGACAGGGAGTATGGGATACTGAAACAAGAGAAATTTATTTACTCATGGTT1470.11193177491814514No Hit
AATTAGGGGATTCAGAGGCGCGGGGCCGGGCCCGGCGGACGCTTGTTGTTGT1460.11117033427244347No Hit
AATTTGGGATTATCAAATTGAATTATTTCTTTTGTTCTTTTATTTTCCCTTC1460.11117033427244347No Hit
TCCGTCGGGAGTGGACATTCTGAGCAAAAGACCACATTTCTGTCCAATTTTG1450.1104088936267418No Hit
CGGCGCGGGCGATCTTAGATAGGGATGAGCATTTCCCTTATGTGTCATATCT1440.10964745298104013No Hit
TATTGTGGGTTCTTGTCTTACCTGCGTAAGTTGAACCCAAAGTTCATAAGAA1440.10964745298104013No Hit
TGTAATGGGACAATACTATGAAATAGGTATTATTATTGCCATTTTCCTGTGC1440.10964745298104013No Hit
GACTCAGGGCTCCAACACTTGACAAAAAACAAAACTGAAGCTGTGATTGGTT1440.10964745298104013No Hit
ACTATGGGGGAGCTCTCAACTCCAAGTTGTAGATTTGGTGTCTTCCTTGTTC1440.10964745298104013No Hit
GATGACGGGCTGGAAGATAGCAGCAGAGTTTTTGATATCTTTGAACCCACTC1440.10964745298104013No Hit
CTTCGGGGGAGTCCAGAAATGTGATGATAGTAGCTTGAGAAGAAAATGCATC1430.10888601233533847No Hit
AAGCATGGAATAAAAAAATTATTTTAAATAATTAAACAGATTTGGAAAAGAC1430.10888601233533847No Hit
TGTCTGGGGAAGTCTTATTGACTTTTAAGAAAGTAAAGATGATAAAAGGTGA1420.1081245716896368No Hit
CAATGGGTGTATTAAAATAGCTTACGGGTAAGGCCTAAAAGGTCACCCCTCG1420.1081245716896368No Hit
TCTTAGGGGCGCTCGCCCCCTTCAGGCCAGGTCCGGGTGGCCAGGGGTACCC1420.1081245716896368No Hit
GGTGTAGGGAAGTATGTCCTCCTTGTGCCAGTTGCAATGATTCTGAGTATAA1410.10736313104393513No Hit
ATACTAGGGCATCTTCCACACTAAACCTTTCTATGGGGGCAAGAAGCACAGA1410.10736313104393513No Hit
ATCACGGGATAGTTTACTATCAACCATAAAATGCATTTTATAATTTTGTAAT1410.10736313104393513No Hit
GTCAAAGGGTATTTTGCAAAGTGCTTTTAAAACATTCTTACTTACCGTATTT1410.10736313104393513No Hit
TCAACTGGGTGTAAGATGCCATTTAGCTTTGAATTCTTCAGAGGAAAAACTT1400.10660169039823347No Hit
TGCATACGGGAGGTTTTAATAAATATCATTTCAGTTTTACAAGATGAAGAGA1400.10660169039823347No Hit
ACGACAGGGAAGACCAATTCAGAGTGTACTTTGGTTATAAGAGAAATTAAAA1400.10660169039823347No Hit
GTCATTGGGCAATTATGTGGGAAATCATTTAATAATATTAAATCTTAGAAGT1400.10660169039823347No Hit
GAACCTGGGACATGTGCATAGACGTTCCTGGGCTATGGAAGATAAAAAATCA1390.1058402497525318No Hit
CTCCTCGGGGAAGTGCCAGATTCTGTTTCATATGGAAGAGAGAATAATGACA1390.1058402497525318No Hit
TGCAAAGGGGAAGTTAACCTGAAATATGTCCACTGGTTACTATAGCCAACAG1390.1058402497525318No Hit
TCTGTTGGGTCTTATGAAACCATGAATGTGAGATGAAGCTAAAGAATAGAAT1380.10507880910683014No Hit
AAGTACGGGCTGCAAGTGCTTCTCTGACATCCTTAGACTAAGTTGAGTACAC1380.10507880910683014No Hit
TGCGCCGGGGATCTTCTTGAGGGAGCGAGCGAGAGGCAGCCGCGCCCGCCGC1380.10507880910683014No Hit
TTTTATGGGATAACTTTGAAAAAAACATAATCTGTTAGATCGGTGAAGGATT1380.10507880910683014No Hit
TTCCGTGGGCAAGCAGCCGCCTCTAACAAACACCATGGTCCGTGGAAGTTCA1370.10431736846112846No Hit
CTAAGTATGGGAGAGCAATAATTAGCCAAATTGCTATATTTCTGGGTTGTGC1370.10431736846112846No Hit
AGTCAGGGAGAGTTTTTAGCATGAAGCGTTGTTGAATTTTGTCAAAGGCCTT1370.10431736846112846No Hit
TAGCCGGGATGGTGAGAAAGTGGTCACCCTGGTCCTGCTGGGTCTTTGCTAA1360.1035559278154268No Hit
CATCAGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA1350.10279448716972511No Hit
AAGCTTGGGATGCCAAACCAAAGAGCAGCTGCAGTACATTCGTTATTTTAAA1350.10279448716972511No Hit
TACTGCGGGGACTCGAGCCAGCTCAAGCCCGCAGCTCGCAGGGAGATCCAGC1340.10203304652402344No Hit
TGCGGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1340.10203304652402344No Hit
TTTTCACGGGGAGATTCTTTAAGTCTACAAAGGTATTCACTGGTTTTTTCCA1340.10203304652402344No Hit
TCATCTGGGAACTTACAGTGTTTTCTTCTCCAGGCAAAATTAATTTCCTAAC1340.10203304652402344No Hit
GCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCA1340.10203304652402344No Hit
TGCTCTGGGACTTTTACCTCTGTAGGAAATGTCTGATGGGAATGGTGCCATG1340.10203304652402344No Hit
CTAAGTGTGGGGATCTGTGGTTGGGGAGGCTGAGCTGGAAAATCTTGAAAAT1330.10127160587832178No Hit
CATTTTGGGGAATGGGAGCCTTCCCCTCACTTCTTAGAATCCTCCTGCAAGA1330.10127160587832178No Hit
CAAGCTGGGAAAAGTAATTACTAAAAAGCACTTAATAGTCTAGAAATCCACC1330.10127160587832178No Hit
TGCAGTGGGAACACACCAAGGAATAAAACCATATAAATGTTCATGGTGTGGG1330.10127160587832178No Hit
TGGATAGGGAGATTGCTTAGAGAAAAAAATAAAATGCTTATCTTAAGGATAT1330.10127160587832178No Hit
CAACAAGGGACAATTTCCTTTTTTCCATTCACCTGTCTTGATGTGCAGAAAA1320.1005101652326201No Hit
TGACTAGGGATATAGAATCCATCTGCAAATTAGGTTCCCCCCACTAGATGCA1320.1005101652326201No Hit
AGCAACGGATTCCAGTTAACTAATTTGTTAACTTTCAACTGTAATCTGTTAA1320.1005101652326201No Hit
TCACTTGGGACTGCAGGATCTTTTCTTCATGAATATATGCTTCCATGAAATT1320.1005101652326201No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)