FastQCFastQC Report
Sat 31 Dec 2022
SRR5847358.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847358.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124794
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAAGAATCTCGTATGCCGTCT4960.3974550058496402No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCAAGAATCTCGTATGC3930.3149189864897351No Hit
TTAGTAGGGGAAGAAAAGTGGCATAGTTGATTGGGCTATTTTGCTCTTAACC1760.14103242143051747No Hit
ATCACAGGGATTCCTGGATGCATTTTGCTTTTTATTAGTTTCCAGATAAAAA1570.12580733048063208No Hit
TACATAGGGACTAGTACAGTAAGGAATCTTGCTGTTTAGAGTAAGCTCAACT1550.12420468932801255No Hit
CACATTGGGATAGGTGCACTGTAGACAATAGATTACAGGAGAGAGATTTTGA1550.12420468932801255No Hit
CAAAAAGGGGAAAAAGTTTATTACCAAACAAGAGTATTTTTATGAAAGAAAA1430.11458884241229547No Hit
AGTTTAGGGCAAGTAAATGAAGGAAAACAGAATTCATTTTCATTATGGGAAA1410.11298620125967596No Hit
CTTGTCACGGGATTGCTTTTGAGCATCCTGAAGAAGTTGATTATATGACGGA1400.11218488068336618No Hit
TAGCACGGGAAGATATAATTTCCAAGGGATTTGACTTTTTTCCTACTCTATC1390.11138356010705643No Hit
GCCAAAGGGAAGCAGCAGGTAGCTTACTTTGACTGGTTTGGAATCGCTTATC1380.11058223953074667No Hit
CTCATCTAGGGATGGCCTGAGAAAGACTTTACACCCTCAAAAGAGAGATAAA1370.10978091895443691No Hit
GGCAATGGGGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.10978091895443691No Hit
AAACCAGGGGATGACCTGCAAACAGATCAAAGATATAGTCAGATATTAAAGT1360.10897959837812715No Hit
AAACTGGGGGATGTGGTAAACAACATCTCTAAAATCTGATAAGAAAGAGAAT1360.10897959837812715No Hit
TAATACGGGATGAACAGATACTTCTCAAAAGAAGACATTTATGCAGCTAACA1350.10817827780181738No Hit
TGGTTGGGGACAGAGAAATATAGCTAAAAAAGCAACAGCAGAATTAAAATGT1350.10817827780181738No Hit
GCCTTTGGGAATGAAAGCTATTTCATGCAAACATTATCTAATTTAGCTTAAA1320.10577431607288812No Hit
CACTGGGGGCTACTTCTGGGGTGTCGGTGATTTTCTATGTTACATATCTAAC1310.10497299549657836No Hit
TTCCAGGGGAGGCATTATGCTGGGTGCTTTACAAAATATAGCTCATTTAATC1310.10497299549657836No Hit
GCGATGGGGATCACACTTCTGAGATAGATTTGAGCCATGGGTGTAGAAGAGG1310.10497299549657836No Hit
CGCTAGCGGGTAATACTCAGTATGCACAAAAATCATGTGTAGCACTTGTAAA1300.1041716749202686No Hit
GATTCAGGGACTGTGAGACCTCACTGTTTCCTTATGCTCATTTTTCCCTTTA1300.1041716749202686No Hit
ATGGCGGGGCACCACTCCTCCAGCCAGAGGGTCTGGACCATCCAGTGCCTGT1290.10337035434395885No Hit
GTTGCGGAGGGAGCCACCACACCTGGCCTTTTTTATTAAAAAAAATTTTTTT1290.10337035434395885No Hit
CATCGCGGGATCATGCTAATTTCTTATCTTTGTAATATTATTCATAAAGAAG1280.10256903376764909No Hit
GCTAGTGGGAAGGCTGATCAGATTACTGGTCACCACCAAATAATTGTTGATT1280.10256903376764909No Hit
CTAGAGGGGGATGAAGAGGGCATCATTGAAGGATTTTAAACAGGAAAGTTTC1280.10256903376764909No Hit
GGTACTGGGAACTGAAATCTTGTCAGGGCAAAGATGTTTAGGGTGAGATGTG1280.10256903376764909No Hit
TCACTAGGGATACATACATAGGAAGGGAATGATAGCTGGCAAAGTCTCTCTG1270.10176771319133932No Hit
TAGACTGGGGACCTTTTTAAAAAAAACTTTTCATTTCATTGGTATTCGTATC1270.10176771319133932No Hit
ACGTCAGGGAGTGATACTCTGCTAAACTTGCAAATGCATTGCAAAAACAGCG1270.10176771319133932No Hit
TCAGACCGGGGTTTTAGAATGTAGTAAGGGCTGAAAGGTGCTCTACTTCTTA1260.10096639261502956No Hit
CAGGAATGGGGGGTGGGACAACACATTTGAGGAACAGCAAGAGGGCCCGTGG1250.1001650720387198No Hit
GGATATGGGTGTTACAGAATGGTGACTTCATTTGTGCTAACAGTACAACAGC1250.1001650720387198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)