FastQCFastQC Report
Sat 31 Dec 2022
SRR5847342.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847342.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25753
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAAGAATCTCGTATGCCGTCT6502.523977788995457No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCAAGAATCTCGTATGC4401.7085388110123092No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGCAAGAATCCTGTCTCTTATAC1020.39607036073467167No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCAAGA620.24074865064264359No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCAAGAATCTCGT620.24074865064264359No Hit
GCTATTGGGGAAAGCAGGAAACATCTAAAATCGACACCGTAACATCACAATT600.23298256513804216No Hit
TGACAGGGGCTATGCTCTCTGTACTTTTGACCAAAAATTGACCAAAGTAAGA580.22521647963344077No Hit
ACGCCGGGTCTGAAGGATCATCTATGTATTCAAGTAATTATTTTTTAGATAA580.22521647963344077No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCAAGAATCCTGTCTCT570.22133343688114007No Hit
GGCTCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA530.20580126587193726No Hit
TGTATAGGGCAACAAAAGATACAAAGAAGGCAGGCATGATAGCACACACCTA520.20191822311963653No Hit
CGCGCTGGGAGGCCTGTTTTTAAGGAAGATAAGCCTTGAAGTGGTGTGGAGA520.20191822311963653No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCAAGAAACTCGTATGC490.19026909486273444No Hit
GGTAACGGGAAGTTTTTTTCTCTTTGAAAGATAGAGATTAATACAACTACTT480.18638605211043374No Hit
ATACAAGGGCTTGCGCCGGGATGGCACATGTATACCTATGTATCAAACCTGT470.18250300935813302No Hit
AAACATGGGCAGTCCCCTTTTGTGTAGGATGATTGCCATCAGTGTTCTGGTT460.17861996660583232No Hit
CGAGCGGGGATGAGGGATGGAGGTCTTGGTGGACAAGTCAGGGGCTCTCCCA440.17085388110123093No Hit
ACGAACCGGGAGTAGGAATCCGGAAGTATTAGATTGAATGGAAAAGCACTTG440.17085388110123093No Hit
AAGCAGGGATTCCACTCACAGCAAAAGACAGGTCATCAAGACAGAAAGTCAA440.17085388110123093No Hit
GAAATCTGGGGCCTCAGCTTTTTAATTTAATTATTTTAATTCTTAATACTTT440.17085388110123093No Hit
GAAGGGGACCATCCTACCTGTGTGTGCACCTTATAACATAGCTGTAAGATAA440.17085388110123093No Hit
TCTCCAGGGATATGAAGCACTCTCTCAGACCACAGTGGAATAAAACTGGAAA440.17085388110123093No Hit
GTTCTTGGGAGTAGATTTTTGTGTCAAATTTCAATCTTTGAACTATAGGATT430.16697083834893023No Hit
ATTAGGGGGAACTGATACCACAAAAATACAAAAGATCATGTAAGGTTACTAT430.16697083834893023No Hit
ACGCTGGGGGATGCACAAGAATCCCAAGGTCCAATTGTGGTCTACTTATCAA420.16308779559662953No Hit
TTTGTTGGGATTCTTTACTTAATAATCATGAACACTTTTCTATCCAAAAAAA420.16308779559662953No Hit
TCTGCTGGGTAGAAGGAAAATATCTCCAGGTCCCATGGGAATGCACAGATAA410.1592047528443288No Hit
AATTCTGGGATATACTTAATTCCTTAATAATCATGTTTCATTTTCTGTTCAA410.1592047528443288No Hit
GCTACTGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT400.1553217100920281No Hit
TTATTAGGGACAAAATGAATTGAAGACTCAAGCATTTGAGGCAGACAATTTA400.1553217100920281No Hit
TTGCAGGGGTGTGGGGGGGCAAACGTGATGATTAACCTCCCTTCTTTGTTTC400.1553217100920281No Hit
ACTAGAGGGGATAATTTGTTGATATATTCATTAGTTGTCCACATTCCCTAAC390.1514386673397274No Hit
AGTAAAGGGGATGCATTCACAGGACCAGGAATATACATGTTCAGAACAGTCC390.1514386673397274No Hit
CAATAAGGGAAGAGGAATTATATTTTTTGATCTGGGCATTTAGTGCTACAAA390.1514386673397274No Hit
CGTTACGGGAACGTATACAGGCAATAAATAGCATGATTAATGGAGTGGTACC380.14755562458742671No Hit
GACACTGGGAACATTTTAGCAAATCGAATTCGACAATACATTAAAAACATTA370.14367258183512602No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCAAGAATCTCGT370.14367258183512602No Hit
CCAATAGGGATCAAAATACAAGCAAGATATTTTTGGAGCTTATTCTAAAATG370.14367258183512602No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCAAGAA370.14367258183512602No Hit
GTTGCTGGGACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG370.14367258183512602No Hit
CATTTAGGGGGCAGTCGCTGCAGCCGCCGCGGGAGGCGTCCGTGACAAGATG360.1397895390828253No Hit
TCGGCAAGGGCCGTCATTTATAGGCAGTGTTACTGTGGGAAGGCTTCTTTAT360.1397895390828253No Hit
ACACCTGGGAATGAAATGAGATGAAATAAAATAATGAAATGATGAAATAATG360.1397895390828253No Hit
TTTACAGGGGAACTAGATGCTACCAACACCAACACAGAACCCCTTTCCCCAT360.1397895390828253No Hit
TAGAGGGGAGAATTTTTTGCTAGCTGACTGTTTCTAAAAGAAATCTAAAGGA360.1397895390828253No Hit
GAAAAAGGGCTGAGGTGCCAAGGCCATGGTGTCCGCCCTGCTGCGTCTGTTC350.1359064963305246No Hit
TGTAAAGGGCAAGCTATTGCATGATTAGCCCCCTATCTGGCAAGGAAACCCC350.1359064963305246No Hit
CTTGCCGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA350.1359064963305246No Hit
TTTGTAGGGAAAGTACTGTGCTATGTGCTCATCGTCATTATCTGTCTGTTTT350.1359064963305246No Hit
TTATTTGGGAACTCAATCTTTTCAAAGAAAAATCCCTTAACAGTGTCGAAAC340.1320234535782239No Hit
TTTTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC340.1320234535782239No Hit
GATCAAGGAAGTGTTCTGGGCGTAAGAACTCCGATTCATATTGCATTCTCTT340.1320234535782239No Hit
TGTGTGGGAAGAAGTATGCGCCTGTGGATGGGGGCCCTGTCACCGCAGCGTC340.1320234535782239No Hit
GCAAAAGGGAACTGCACTGGATCCTTGAAAATGGAGCTTTGGCTTCAACTTG340.1320234535782239No Hit
TAAGTAGGGACTGGTACCTAAAAAAGGTACTAGGTTAAAAATGTAAGACAAT330.1281404108259232No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCAGCAAGAATCTCGTATGCC330.1281404108259232No Hit
GTCGAAGGGGTATCCCTCATGCAGTTGGCATCCAGGCAGCCCTCTGCAGCAG330.1281404108259232No Hit
AGTTATGGGCTTTGCACACATTTTAAAAATGTGATTATCTTCATTTTGCAGA320.12425736807362249No Hit
CAAGTGGGGAGAACTGGATGATAGATTATGTGACGCCTGAAGTGGATCCAAG320.12425736807362249No Hit
ACAGCCTTCTATCAATTTGATTCATTTCAGTTAGTTTGGCAGAAGTGGGCTG320.12425736807362249No Hit
CATCCTGGGAACTTTGTAGTCTACTAGCACAGTGCTTGCTGGAGGCTTTGGC320.12425736807362249No Hit
GGCGCAGGGATATCTATCTTTATTTTCTTTCAAATGCCCCATTGGAATAAAA320.12425736807362249No Hit
CTTGCTGGGATTACAGGCGTGAGCCACCGCGCCCGGCCAGAGATGTTGACTT320.12425736807362249No Hit
TTCGCAGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGTG320.12425736807362249No Hit
TATTGTGGGAGTCCGTGTGCGGGGAGGTAACAAGAAATACCGTGCCCTGAGG320.12425736807362249No Hit
CAACCTGGGAGCTCGTTGACAAGAAAAACAGCCTGGTGGCTGAGGAGGCCGA310.12037432532132179No Hit
TCTCATGGGATCCTCCAAAATGATTTATTCACCTATTAGAGCTTCTGCTTCT310.12037432532132179No Hit
CAAATAGGGAGCTCCAGCCTCAAACAGCATCTGTACCGGTCCATCATCAACT310.12037432532132179No Hit
TGTGACGGGAAGCTACCGATTTATGGATATGTTTCCAGATGCCAGCCTTATT300.11649128256902108No Hit
TTTTTAGGGCTTGGTAGATGATGTCTTATAAATATGACCAGACTACAATAAT300.11649128256902108No Hit
GCTCGAGGGCAAGAGCAGCTGGGAGAACGTCCGAGCTGCTGCACCCCTGTTC300.11649128256902108No Hit
ATATACGGGCAGTGGCTTCAGAGGAAGAGCAAGAAAGGCATGAAAGAAGTGA300.11649128256902108No Hit
TCTAATGGGACATTTTTTTCAGCACCACACCACACCTATTCCAAAATTGACC300.11649128256902108No Hit
CATACCGGGGAAACCTCTTCAAGTTCTTTATTGTTTGGGACTGAGACACTCA290.11260823981672038No Hit
GAATGGGGGAGACTGTGGGAAAATATATAAGATAAACAACCCAATTTCTTCA290.11260823981672038No Hit
CATATAGGGAATCTCTGTGTTAATGTTTCATTTGTCAAACTAAATCATCTCA290.11260823981672038No Hit
CATAAAGGGGAACTGAACAGCAGTTCCTGAGCTTACGAAGGGCTGGGAATTG290.11260823981672038No Hit
GTGCAAGGGAGTCATTGACAATCTGACACATCCATGATAAAGTTGCCTGACA290.11260823981672038No Hit
TATTGTGGGAAATTCTAAGGATAATAAATTTTTGACCTGTACAGAAGAAGAT280.10872519706441967No Hit
ACATCAGGGCAGTGTGGCGATTCCTCAGGGATCTAGAACTAGAAATACCATT280.10872519706441967No Hit
GGCAGTGGGGGTTTCCTTCTGATAAGGGATACAGTGGACCATGTCGCTTATA280.10872519706441967No Hit
GACAACGGGAATAAGTCCTCAAAAGCAAACACAATAAAAACAAAAATAGATA280.10872519706441967No Hit
GGCTGTGGGGGGCCTCAGTCCCATGTTGGGAGCAGGACAGGCTGACCAGGCT280.10872519706441967No Hit
AAGATAGGGGAATAGGAACCTGTTAGGGGAGCCCAAGAGCATAGTTGAAGAA280.10872519706441967No Hit
AGAACAGGGGAATACACAGGAAAAGTTCTTGAAGAAAATTAAAAGTGCTGCT270.10484215431211899No Hit
ATGCGAGGGATTAGTATGGTCTTAATTGATTCAGGTAATTAGTGTCTCTCCC270.10484215431211899No Hit
AACCACGGGTACCTAAAATTTGAGGACAACAACACCTGAGTTATTGTATCCA270.10484215431211899No Hit
TACTGTGGGATGCCTGGAAGGCCAATGCCATAGGTCGGGGTGCCAAGTCAGT270.10484215431211899No Hit
TCTGCAGGGATTGTTGTTAACTGTAGTCACCACGTTGTACTGTGGATCTCTT260.10095911155981827No Hit
CAAGTAGGGGCCATCCACAGCTAATGAAGATATCCATAAAAGGATAGCACTG260.10095911155981827No Hit
GTTTCAGGGACTTAACAGAAATGTTAAACTATAACAAGCACCATTTGAGGAT260.10095911155981827No Hit
TGAGCAGGGATGGGGGTTTCTAAATATATCAACAACATGAAGTCAATAGGTT260.10095911155981827No Hit
CTTGCTGGGATGGACCATTTGGTATAGGAGGAGTGAATAGTTGAATATTGGA260.10095911155981827No Hit
TTAGCAGGGAAGGAGGATGGGTGCCTGACAAGGACATGTTACCAGGCGGTCT260.10095911155981827No Hit
AGAACTGGGGGTAAGGGCTGACTGAGATAATGCATCTGCAGTGCTCTACGTA260.10095911155981827No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGCAAGAAT260.10095911155981827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)