FastQCFastQC Report
Sat 31 Dec 2022
SRR5847319.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847319.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202087
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT8480.41962125223294916RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC8340.4126935428800467No Hit
TGGCCAGGAATGCTGCGGGACAGTCTACTTTGATTGCTTTCTTTCCTCCATG3130.15488378767560507No Hit
AAGCGAGGGGTGTGCATGACTTTAATGACTTTTTTAATGGGGTGGGAGGTGG3050.15092509661680367No Hit
AGTTTAGGGGCAGAAAGTCTCCAGACTGAAGGGTTTGAACATCACCTTATAA3030.14993542385210332No Hit
TTCTACGGGAAAAATTAAGGAAGTATCTTAGTAAAATAAGAAGACAAAGTCA3020.14944058746975314No Hit
ATCGCGGGGATTGGAGTAGCGAGGAATCTGATTCCAAGCAAAAACCAGGCTC3010.14894575108740296No Hit
CTGTCGGGGAGGGGAATATGTCACATTAAATACAAAGGGAATTTTTGTAGGT2990.1479560783227026No Hit
TTTAGTGGGGGCGTCCCGCAGGAGCCGCAGGCAGAGCGTCCGGCGGCCGGGA2990.1479560783227026No Hit
TTTCAGGGGCAGGTCAAGCATTTAAACCCTGAAACAAAAGTCAGAACAAACT2940.14548189641095172No Hit
TATGTTTGGGGAAACAAAACAAAACAAAACAAATAAACAAACAAACACGTTC2920.14449222364625136No Hit
TTCAGTGGGATGCTGGTAGGGCAGGGGTGAAATATAAACAAGTAAATACACA2910.14399738726390118No Hit
TAGACTGGGATCATCTTATAAGTGAGCACCTTCCCTGAGACTCTTTAACTTC2810.13904902344039943No Hit
TCAACCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2760.13657484152864854No Hit
GTCTGGGGCAGAAGTGAAAGATGAGAAGCTTAATGTAGAGTAAAATGTCTGA2720.13459549599924783No Hit
TCATATGGGACCTTTCTCAGAGGTATTTGCATTTTAAATGAGGGTGGCTCTC2700.13360582323454748No Hit
GTGCTCGGGCAAATGGCCTAGACATGGTTAATGCAATTTGTTGCTAGTGGAA2650.13113164132279662No Hit
GTAGGCGGGCAGAGAGTTCCACTGAGAAGGCAGAAGAATAAGGTGCCCATAG2620.12964713217574608No Hit
AATCGCGGGATAATCTCATTTTGGTTCACTGAGGAAAACCCTGCTTCACAAG2620.12964713217574608No Hit
ATAGCTGGGATTGTAGGCGTGCGCCACCACGCCCGGCTAATTTTTGTGTTTT2610.1291522957933959No Hit
TATCCGCGGGTGGCTCACTGCAACCTCTGCCTCCCGGGTTCAAGTGATTCTT2610.1291522957933959No Hit
ACTTGTGGGCTTGGATAGATCCTAAAAGAAACAAAAAAATTACCTTTGAAGA2590.12816262302869558No Hit
GCACTAGGGGGAGCCAAATAACACATGTTATAAACAGGAAATATCTTCCTTT2590.12816262302869558No Hit
AACAACGGGGCTACTGGTTTTCTTCCTTCTACTCTGCCTACTATCTTCTTTC2590.12816262302869558No Hit
CAAAAAGGGATTTCTATGGGTGGGTCTAATTTGGTAACGGTTTGATTGTGCC2580.1276677866463454No Hit
TTCTATGGGATTGGCTCCTTCGTACTTCTTTTACCACCTCTATTTATTAATT2580.1276677866463454No Hit
GTCTGCGGGATTGTAACAGACATTGTTTTGCCAACATTGCCTATTTCAGTGG2560.12667811388164504No Hit
TAGTAAGGGGTGGGTAAAAAAATGAAAGCATTTTGGCTGGTTAACAAATCAA2550.12618327749929487No Hit
TGATCCGGGATCCCTACTACATGGGGCTTATACTGTAGTTGTGAACCCAGGA2550.12618327749929487No Hit
GAATCTGGGGGCTTGTAAAATGTGACATGTTAACATTTTTCTCCTTAACCTC2540.1256884411169447No Hit
TAGAATGGGACTTCTAGAGATTTTTGAGAATTGTAGCATCTTCCATGTGAAT2540.1256884411169447No Hit
TGCTGTGGGGAGAGTGAGGTTCAGGAAAGCAGCCTAAGCTGAAAATATTGTT2530.1251936047345945No Hit
AACAACGGGAACTAATTAATATTAAAGACAGAATTGAAAGATACCTTGTTAT2520.12469876835224433No Hit
TCATACGAGGGGCGCCGCTGTGGGAGAGACCCCAGCAGGAGCCCAAAGGCAG2520.12469876835224433No Hit
GACGCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2510.12420393196989415No Hit
CAATACGGGGTTCTACTTTAATTCCTTTTTTAGGGAACTACAACCATCAAAT2500.12370909558754398No Hit
TTGCTGGGGTGCCCGAGAAAATGAAATGGATGAAAACCTAGAGCAGGTGAGC2480.12271942282284362No Hit
ACAATAGGGAGAATTGTCTTGTTGAACATATGTATTTCTGCTGGGTATAAAG2440.12074007729344294No Hit
GTTAATGGGACCCCTGCCCAGCAGCCGGGCCGTGGTCTGGCTGAAGCTGCGG2440.12074007729344294No Hit
ATTTATGGGATGGGGACTCATTTTATGATGGACAACTTGAGAAAATTGAGTT2430.12024524091109276No Hit
CATTGTGGGTGTAAGGGCAGACCTGCATCTGTGTGTGTGTTTTCAGTGGGTG2420.11975040452874258No Hit
GAGTGAGGGGCTGTCTATTTTTCATGTTATCAGTTTCTTTTGGTGCCTAGTT2410.1192555681463924No Hit
GCATTTGGGACATCCCAGACATTAGGAGTTCATAAGTGTATTTAATGAAATT2400.11876073176404223No Hit
TCTCAAGGGGGCAGCTTTTAGGCTGCAGAGTGATGAGCCTCGAAGCAGAATT2390.11826589538169205No Hit
AAACTTGGGCTTGATACATAATGCCAAATTATTCTTCATAAGAGCTGTTGCC2390.11826589538169205No Hit
TGTTTAGGGTTATTGCCATTTTACAGATGAAGGGACCAAAGCTTGAAGAGTT2390.11826589538169205No Hit
GGCATGGGGTACCATGAGGAGCAACAGTGATAATTTATTTACTTATTTATTT2390.11826589538169205No Hit
ATAGGAGGGTGGTGTGAGATGGTATCTCATTGTGGTTTTGATTTGCATTTCT2380.11777105899934187No Hit
AAAAATGGGCTGTTATTCACATCTAATATTAATATGGTGTATTCGTTAGAGT2380.11777105899934187No Hit
ACACCTGGGGAGTAGTCAAATTCATAGAGACAGAAAGTAGAACGGTGATTGC2370.1172762226169917No Hit
ACTGCTGGGAAACTCATAATCTTCTGAGATTACAACTTACGACTCTTCTTTG2370.1172762226169917No Hit
TGATCAGGGCAGCTGGTTATTGGTAGAGCTGTCACCCCCTAGTTCTGACTTC2370.1172762226169917No Hit
GCAAGTGGGGGGGAAAAGGCTCTTAGAGCTTTTTAGTGGACTGACACATAAT2360.11678138623464152No Hit
AACGGCGGGCTGATGAACACTAAATATTAATTTCAATTAGCTAGATTGTACA2360.11678138623464152No Hit
GGACTTGGGAAATTCAGTTTATTAATAGCCAGGTATATCCTGTATATCTCCA2350.11628654985229134No Hit
GATTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2340.11579171346994116No Hit
TTGGGTGGGCATCTATGGGTTCTGGTAGTCCTGGGAGAGGCAGGGAAATGTC2340.11579171346994116No Hit
CTGTCAGGGCAACTGCTGCTTGCAAGTTTACTGGACAGAAACTGTTATTCAA2340.11579171346994116No Hit
TTTTCAGGGAACTTTATAAACATAATTCAACCACATGGGAATTCTGAGTAAA2320.1148020407052408No Hit
TCGGCCGGGTCTGCTTCCGGGGGAATCTGTAACATAATAAATTTTTTGTCTT2310.11430720432289063No Hit
GCTTGGGGGGAGAAATGAGTTGGAAAGCTATGCCTATTCTCTAAAGAATCAG2300.11381236794054045No Hit
CGGTTTGGGAGAGAAGGGGTTCTATAACTGCTTTTGTTTTGTTTTGGATAAA2300.11381236794054045No Hit
TAGTGCGGGATCATCAGTCATTACGACACAAAAACCAAAAGCAGAATGAGAC2300.11381236794054045No Hit
ACTTCAGGGTTAATGACAGGCCTGCCAGAGCCTCTTTGTTGCTTTCAAAAAG2290.11331753155819027No Hit
GACTTTGGGGCAAGGACCCCGGTTTCTTCGTCGGAGGTCCGCGCAGGGCTCT2290.11331753155819027No Hit
TTCAATGGGATGTAGACTGTACCCCTAAAGCACACATCTTTGTCTTTGAATA2290.11331753155819027No Hit
ACCGCGGGGGAATAGACTTCCTAGCTTATTCAGAGTGTTGATAAAACTTTGT2280.11282269517584011No Hit
GGCTCTGGGCGGTTATGTTGTGTAAGTCAACTCTTCAGCCTCATTCACTGTC2270.11232785879348993No Hit
CTACTTGGGCCGATCTAAGTAAGGGCTACCAGGCTTCGGAATAATCTCCCAT2270.11232785879348993No Hit
TGCAAGGGCCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTG2270.11232785879348993No Hit
TCGAGAGGGATCTGACCTGGAATATAAAAATACTTTGTTAAGTAAGATACAT2270.11232785879348993No Hit
GCACAAGGGCGATCTTGCACCGGGGCTTTTCTTCCTTTTGGTGCGAGCTTGC2260.11183302241113975No Hit
CTCATTGGGATCCACATCTAGAAAGGGCTGCTCACTGCACCAGAAAGACAGT2250.11133818602878957No Hit
TGATAAGGGGTTAGATAAATATCTCAAAGTGAGATTTCTGAGCAGAGTGTAT2250.11133818602878957No Hit
TGTAACGGGAGATTCTCGGATTCTCGACTCTTTGCAACAGCACTTGCTTGCG2240.11084334964643941No Hit
AGTTGCGGGCTACAGGGGGTATTATAACTTTGTTATGTGTTGTGTATGTGGC2230.11034851326408923No Hit
GAAATAGGGACAGGCTAGGTTCCTTGTAGCATGTGGGGGTTTCTAGAGGGGA2220.10985367688173905No Hit
CAGCTGGGGGGCCTATATCGATTTTCTAGGATCATTATTTTGTTTGTTGTTT2220.10985367688173905No Hit
TTTCCGGGGCCTACCTTCCATACCAAGTCACTGTGCATCCTACATGTATTGA2210.10935884049938889No Hit
TATTTTGGGCGCTCTTTCGATCTTATTTTGGGAGCCACGGCGGGGGTGGCTG2210.10935884049938889No Hit
GGCACTGGGGAAAATGCTAAACTGAAGAAAGAAATTGAAGAACTGAAACAAG2210.10935884049938889No Hit
ACCTAAGGGAGACTTTGTCAAATGGACTGGTCCTGGCAGATTCATAGCCACA2200.10886400411703871No Hit
TTATACGGGAGGCAGACAAATTTGGCTTCAGATCCTGTTCCTCTGATTAGCT2200.10886400411703871No Hit
GCTAAAGGGGAATGCTCAAGGCATTTTGCTTGTTGACTTTCTGTAGGGCCAA2200.10886400411703871No Hit
ACAGACGGGGATGATTATAGAGTTCCCCAAAATGGCCTTCTTACTATTTCTA2200.10886400411703871No Hit
AGTTGTGGGTGGCATTCCTTGACTAAAAAGGACCACCTGATGCTCAGTCCCG2200.10886400411703871No Hit
CTAAGCGGGATGAACTTTATGCTTCACTGTTAGTTGTTTGTGGAAGTTATTT2200.10886400411703871No Hit
GCTCATGGGGAAGCTCACTGGCATGGCCTTCCGTGTCCCCACTGCCAACGTG2190.10836916773468853No Hit
CAGCACGGGCACAGGCTCTGCTTCCCTGCCACCTTGGGTAGCTCCTGCCTTT2190.10836916773468853No Hit
ACTAATGGGATTGCATTGCAACAGGTCGACACATGTTGAAAGATGACTGGAC2190.10836916773468853No Hit
TCTCACGGGCTTGCCACTTTGGGAGGCTGAAGTGTGAGGATTGCTGGAGGCC2190.10836916773468853No Hit
GCTCTAGGGTATGAGCTCAGGAGTTCGAGACCACCCTGGGCAACATGGTGAA2190.10836916773468853No Hit
AAGCCTGGGGAAGAGCATCTTGGGCCCTGAGTTAATTTAATAGATCATGGAG2180.10787433135233836No Hit
TTGCCGGGCCTGCTTATCTTTGTTTGACATTCTGCAATTTATCAATAATTTG2180.10787433135233836No Hit
GTCAGGGGGGACTTTCGGGTCTTGTTTTTGTATATCCTTTATGAGAATAATA2170.10737949496998818No Hit
GTTAGTGGTCAGAATCAGGAAAGTAAAGTTTTAAGGATTGACATGAAAGGTT2170.10737949496998818No Hit
TTACGAGGGTAGTGGTCATTTGGTGGCTCTAACTCCTTGAGGTCTTGAGTTT2170.10737949496998818No Hit
ACCTAAGGGGAAGATATTGAAAAAACTGGATATATTTTTATTAAATTGTGAT2160.106884658587638No Hit
GAGTTTGGGGGCCCGCGCGGTGTTCCGCATTCTGCAAGCCTCCGGAGCGCAC2150.10638982220528782No Hit
TCCAGCAAGGGATGGCCCACAGCTCCTGTGCTGGCCAGACTCCCAGAAAATT2150.10638982220528782No Hit
AAACCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2150.10638982220528782No Hit
CTTTCCGGGCTCTGGAATTGTTGAATGTATTCTTTTATATAACTACATTAAA2150.10638982220528782No Hit
AATTCAGGGATTTCTGGAATGCAGAAAACATTTTAAAGGCTAGATTTTTAGA2150.10638982220528782No Hit
AGGATTGGGATCCCTTTGCAGCAGTCTTATTTCTGATGCACTTAAAGAATTT2150.10638982220528782No Hit
GCACATGGGATGCCTCTGATCCTTTGCTCTGAAAACAAAGCCTAAAACAACT2150.10638982220528782No Hit
TTCACTGGGAGGATATTTTGAACCAATAGAATTCTAGGTTGGCAATTATTTT2150.10638982220528782No Hit
CGCTCCGGGATGTGGACCAAGGTCAGGTTAGTTAATTTGTTTGCTTTTATTG2140.10589498582293765No Hit
CATCTCGGGAACGCTGGCAACATCGTGGTCAGTAGGCTGGACCCCGTGTCCC2130.10540014944058747No Hit
ACTAAAGGGCTGGCCTACTGTTTTGGCTGGCATCTGGATGGACTCCAGCATT2130.10540014944058747No Hit
GCTGTGGGGTATGTATTTTGCAGATGAGCTGACCTCTCCCATTCCTGCAACC2130.10540014944058747No Hit
GCATAGGGATGACATTTAATTTCACTGTATTCAGCTTTAAGTTGTTCACAAT2120.10490531305823729No Hit
GTTGCCGGGATTGCTTAATCTTTTATATGGAATTTCACTTTATAAAACAAAG2120.10490531305823729No Hit
TATGTAGGGCCAAGGCAGTTGGACCTTTGCTACTACCACTGCCCTGAAACTG2120.10490531305823729No Hit
TTTTCTGGGGTTATTCCCCCCTACTTGAGATAATCTAAATATAAACCAGCTA2110.10441047667588711No Hit
AACGATGGGGGAAACCAAAACACCTACCCTGCAACCAACTCCTGAAGTTCAC2110.10441047667588711No Hit
ATTGGCGGAGACGTTTTTTAAAATCCCGATATGAACAACCGAAGCCCTCCAC2100.10391564029353693No Hit
TTAGCGGGATAACATTTCTTCTTTCAACAGAGTATATGTTTCCCATTTAACC2100.10391564029353693No Hit
GACAGTGGGATTCTAACTGATACGTAGACACTTACTTGGAAATTTTTGGACA2100.10391564029353693No Hit
ATCGCTGGGAACTTTGCTCCCACTGGTTTAATAGTTACTTATTTCTGCCTAA2090.10342080391118676No Hit
CAATAAGGGATGGAAGGGGCCAGTTGCTGTTTTTTCATGCAGTGCCCTGGGA2080.10292596752883658No Hit
GTATATGGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAG2080.10292596752883658No Hit
TGCAGAGGGAGAAAGACCTTGGGCTTTGGGCTTAAATTGCACCTGCAGCATT2080.10292596752883658No Hit
CATCGTGGATCCAGCTTTTTCTTGTCATTTTAATAAGAGTATGTGTCTTATA2080.10292596752883658No Hit
AGCATGGGGGCCTGTTTTTTCTTGATACTTACTGAAATAGAAGGTTTTATTG2070.1024311311464864No Hit
ACTTCTGGGAGCTCATAGTATGCAGAAATATGAATAAGGTAGAATATCTGTC2070.1024311311464864No Hit
TTCGCCGGGAAGGTGTTTTGCAAGAGCTGTATTTATATTACAGTTTTTTAAA2070.1024311311464864No Hit
AAACAAGGGGGACCTACTTCCTTTATATAAAAATGAATATATAATATATATC2060.10193629476413624No Hit
GTTTGAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2060.10193629476413624No Hit
TATGCAGGGGGTGAAATAATCTAGCCAAATATGTCACATATACATGTTCCAA2060.10193629476413624No Hit
TCACCAGGGGAAGTGTTTCTTAATGTACAAAATGGAGAATTTACAGTCTTTG2060.10193629476413624No Hit
CACCAAGGGGCTCTTCCGATCTACTTGTGGGAGAACAAGCCATCGTCATCAT2050.10144145838178606No Hit
TCAACTGGGCGACCGGAGCCGTCTTCCCAAATCGCCACCCTACACTGCTTTT2050.10144145838178606No Hit
TTAAGGGGGGTCCATAAATCTGAGACTTGATGTATTTTTTCATTTTGTCCAG2050.10144145838178606No Hit
AATCACGGGACTGTTAATAAGGCCAGGAAAACTGCCATTTCAAGTTCTGAAA2050.10144145838178606No Hit
AGTCGAGGGGGGCCCAGGCCGCCCCCGCCGTGGGGCCCAGGAGCCAGAAGCA2050.10144145838178606No Hit
TTCGCGGGGAAGTACATTCAAGTACATCCCCATGTCCCACATACACCTGAGG2040.10094662199943588No Hit
CTCGCGGGGGTGGAAGTCCTAGCTAGGGCAATCAGACAAGAGAAAGAAATAA2040.10094662199943588No Hit
AGCCGCGGGCGGGACAACTCCACCATGGGCTATATGATGGCCAAAAAGCACC2040.10094662199943588No Hit
TGTCCAGGGGAAACTGAGGCAGGATTTCCTGCCTCTAAACTATTGGCAGCAA2040.10094662199943588No Hit
TATGCTGGGTTATAAAATATATATTTTGCCAATAATATAAAAATAGATTGAG2040.10094662199943588No Hit
TGTCGTTGGGCAACTGGTAGTCCATTAAATTCTCCTGTCTAGAATGACCCCC2040.10094662199943588No Hit
CTTTGAGGGAAATCATTTTCTTATATTGTTATATGCTATATATGTTCTTAAG2040.10094662199943588No Hit
AATACTGGGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTT2040.10094662199943588No Hit
TAGCGCGGACGAGGCTCCTGCTTACCATCTTATTTTGGAAGGGATTCTGATC2040.10094662199943588No Hit
GAATTAGGGCTGCTGCTTAGATAATGTCACTGTTAGCATAATGATATATGTT2040.10094662199943588No Hit
GGTTCAGGGTGTTTGATTATTTCAGGATCTGAGCAAATGGTACATTTTAAAA2030.10045178561708572No Hit
GACTATGGGTGGATTAAGGTGAGTGAGTGCACCTGTGACAGAACAAGTGCTC2030.10045178561708572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)