FastQCFastQC Report
Sat 31 Dec 2022
SRR5847318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202632
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC8580.4234276915788227No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT8560.42244068064274154RNA PCR Primer, Index 23 (95% over 21bp)
TTCTACGGGAAAAATTAAGGAAGTATCTTAGTAAAATAAGAAGACAAAGTCA3210.15841525524102806No Hit
AGTTTAGGGGCAGAAAGTCTCCAGACTGAAGGGTTTGAACATCACCTTATAA3160.15594772790082514No Hit
AAGCGAGGGGTGTGCATGACTTTAATGACTTTTTTAATGGGGTGGGAGGTGG3160.15594772790082514No Hit
TGGCCAGGAATGCTGCGGGACAGTCTACTTTGATTGCTTTCTTTCCTCCATG3040.1500256622843381No Hit
TCATATGGGACCTTTCTCAGAGGTATTTGCATTTTAAATGAGGGTGGCTCTC2950.14558411307197286No Hit
ATCGCGGGGATTGGAGTAGCGAGGAATCTGATTCCAAGCAAAAACCAGGCTC2930.14459710213589166No Hit
ATAGCTGGGATTGTAGGCGTGCGCCACCACGCCCGGCTAATTTTTGTGTTTT2920.14410359666785108No Hit
TTTCAGGGGCAGGTCAAGCATTTAAACCCTGAAACAAAAGTCAGAACAAACT2870.14163606932764816No Hit
TTTAGTGGGGGCGTCCCGCAGGAGCCGCAGGCAGAGCGTCCGGCGGCCGGGA2840.1401555529235264No Hit
TGTAACGGGAGATTCTCGGATTCTCGACTCTTTGCAACAGCACTTGCTTGCG2810.13867503651940463No Hit
TATGTTTGGGGAAACAAAACAAAACAAAACAAATAAACAAACAAACACGTTC2790.13768802558332346No Hit
GCAAGTGGGGGGGAAAAGGCTCTTAGAGCTTTTTAGTGGACTGACACATAAT2760.1362075091792017No Hit
TAGTAAGGGGTGGGTAAAAAAATGAAAGCATTTTGGCTGGTTAACAAATCAA2750.13571400371116113No Hit
GTCTGGGGCAGAAGTGAAAGATGAGAAGCTTAATGTAGAGTAAAATGTCTGA2740.13522049824312052No Hit
TTCAGTGGGATGCTGGTAGGGCAGGGGTGAAATATAAACAAGTAAATACACA2730.13472699277507993No Hit
TGCAAGGGCCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTG2700.13324647637095818No Hit
AATCGCGGGATAATCTCATTTTGGTTCACTGAGGAAAACCCTGCTTCACAAG2690.1327529709029176No Hit
GTGCTCGGGCAAATGGCCTAGACATGGTTAATGCAATTTGTTGCTAGTGGAA2690.1327529709029176No Hit
TTTTCAGGGAACTTTATAAACATAATTCAACCACATGGGAATTCTGAGTAAA2670.13176595996683643No Hit
TCATACGAGGGGCGCCGCTGTGGGAGAGACCCCAGCAGGAGCCCAAAGGCAG2670.13176595996683643No Hit
ACCTAAGGGAGACTTTGTCAAATGGACTGGTCCTGGCAGATTCATAGCCACA2660.13127245449879585No Hit
TTGCTGGGGTGCCCGAGAAAATGAAATGGATGAAAACCTAGAGCAGGTGAGC2660.13127245449879585No Hit
GACTTTGGGGCAAGGACCCCGGTTTCTTCGTCGGAGGTCCGCGCAGGGCTCT2640.13028544356271468No Hit
TAGTGCGGGATCATCAGTCATTACGACACAAAAACCAAAAGCAGAATGAGAC2640.13028544356271468No Hit
AACAACGGGGCTACTGGTTTTCTTCCTTCTACTCTGCCTACTATCTTCTTTC2620.12929843262663349No Hit
TGATCAGGGCAGCTGGTTATTGGTAGAGCTGTCACCCCCTAGTTCTGACTTC2620.12929843262663349No Hit
ACTGCTGGGAAACTCATAATCTTCTGAGATTACAACTTACGACTCTTCTTTG2590.12781791622251173No Hit
GGCTCTGGGCGGTTATGTTGTGTAAGTCAACTCTTCAGCCTCATTCACTGTC2580.12732441075447115No Hit
GCTCATGGGGAAGCTCACTGGCATGGCCTTCCGTGTCCCCACTGCCAACGTG2580.12732441075447115No Hit
CTGTCGGGGAGGGGAATATGTCACATTAAATACAAAGGGAATTTTTGTAGGT2580.12732441075447115No Hit
GAAATAGGGACAGGCTAGGTTCCTTGTAGCATGTGGGGGTTTCTAGAGGGGA2570.1268309052864306No Hit
TATCCGCGGGTGGCTCACTGCAACCTCTGCCTCCCGGGTTCAAGTGATTCTT2570.1268309052864306No Hit
ACACCTGGGGAGTAGTCAAATTCATAGAGACAGAAAGTAGAACGGTGATTGC2530.12485688341426823No Hit
GCACATGGGATGCCTCTGATCCTTTGCTCTGAAAACAAAGCCTAAAACAACT2530.12485688341426823No Hit
TGTTTAGGGTTATTGCCATTTTACAGATGAAGGGACCAAAGCTTGAAGAGTT2530.12485688341426823No Hit
GAGTGAGGGGCTGTCTATTTTTCATGTTATCAGTTTCTTTTGGTGCCTAGTT2520.12436337794622765No Hit
AACAACGGGAACTAATTAATATTAAAGACAGAATTGAAAGATACCTTGTTAT2510.12386987247818707No Hit
ATTTATGGGATGGGGACTCATTTTATGATGGACAACTTGAGAAAATTGAGTT2510.12386987247818707No Hit
CAATACGGGGTTCTACTTTAATTCCTTTTTTAGGGAACTACAACCATCAAAT2500.12337636701014647No Hit
GAATCTGGGGGCTTGTAAAATGTGACATGTTAACATTTTTCTCCTTAACCTC2500.12337636701014647No Hit
ACTTGTGGGCTTGGATAGATCCTAAAAGAAACAAAAAAATTACCTTTGAAGA2480.12238935607406529No Hit
GCTTGGGGGGAGAAATGAGTTGGAAAGCTATGCCTATTCTCTAAAGAATCAG2480.12238935607406529No Hit
ACAATAGGGAGAATTGTCTTGTTGAACATATGTATTTCTGCTGGGTATAAAG2470.12189585060602472No Hit
GGCATGGGGTACCATGAGGAGCAACAGTGATAATTTATTTACTTATTTATTT2470.12189585060602472No Hit
ATAGGAGGGTGGTGTGAGATGGTATCTCATTGTGGTTTTGATTTGCATTTCT2460.12140234513798413No Hit
CTCGCGGGGGTGGAAGTCCTAGCTAGGGCAATCAGACAAGAGAAAGAAATAA2450.12090883966994354No Hit
GCACTAGGGGGAGCCAAATAACACATGTTATAAACAGGAAATATCTTCCTTT2440.12041533420190295No Hit
GACGCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2440.12041533420190295No Hit
TGATAAGGGGTTAGATAAATATCTCAAAGTGAGATTTCTGAGCAGAGTGTAT2440.12041533420190295No Hit
CTATATGGGCAGGGGTTTGAAGCACACAGGAGCTGGACATGTCAATTCTGTA2430.11992182873386238No Hit
CGAATAGGGAATCCTGGTGGAAAAGCCACAGAACCTTCTTCCTGCCCTTCTT2420.11942832326582177No Hit
TCAACCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2410.1189348177977812No Hit
TTCTATGGGATTGGCTCCTTCGTACTTCTTTTACCACCTCTATTTATTAATT2410.1189348177977812No Hit
GCATTTGGGACATCCCAGACATTAGGAGTTCATAAGTGTATTTAATGAAATT2400.11844131232974062No Hit
TTCAATGGGATGTAGACTGTACCCCTAAAGCACACATCTTTGTCTTTGAATA2400.11844131232974062No Hit
CGGTTTGGGAGAGAAGGGGTTCTATAACTGCTTTTGTTTTGTTTTGGATAAA2400.11844131232974062No Hit
GTTTGAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2390.11794780686170002No Hit
TAGACTGGGATCATCTTATAAGTGAGCACCTTCCCTGAGACTCTTTAACTTC2390.11794780686170002No Hit
CAATCGGGATTCTTAAAAATTGAAAACATAAGCTAAAGAGATGACTAAATGG2380.11745430139365944No Hit
GTCTGCGGGATTGTAACAGACATTGTTTTGCCAACATTGCCTATTTCAGTGG2370.11696079592561887No Hit
AACGGCGGGCTGATGAACACTAAATATTAATTTCAATTAGCTAGATTGTACA2370.11696079592561887No Hit
TCTCAAGGGGGCAGCTTTTAGGCTGCAGAGTGATGAGCCTCGAAGCAGAATT2360.11646729045757825No Hit
CATTGTGGGTGTAAGGGCAGACCTGCATCTGTGTGTGTGTTTTCAGTGGGTG2360.11646729045757825No Hit
TTCACTGGGAGGATATTTTGAACCAATAGAATTCTAGGTTGGCAATTATTTT2360.11646729045757825No Hit
TCCAGCAAGGGATGGCCCACAGCTCCTGTGCTGGCCAGACTCCCAGAAAATT2350.11597378498953768No Hit
GTTGCCGGGATTGCTTAATCTTTTATATGGAATTTCACTTTATAAAACAAAG2330.1149867740534565No Hit
GGCACTGGGGAAAATGCTAAACTGAAGAAAGAAATTGAAGAACTGAAACAAG2330.1149867740534565No Hit
TCGGCCGGGTCTGCTTCCGGGGGAATCTGTAACATAATAAATTTTTTGTCTT2310.11399976311737535No Hit
TCAAGGGTTATGTAAACTGGAAAAAATATTTGACTAGTCTTTTTTTCCTGAT2310.11399976311737535No Hit
TTATACGGGAGGCAGACAAATTTGGCTTCAGATCCTGTTCCTCTGATTAGCT2290.11301275218129417No Hit
TGCTGTGGGGAGAGTGAGGTTCAGGAAAGCAGCCTAAGCTGAAAATATTGTT2290.11301275218129417No Hit
ACTTCTGGGAGCTCATAGTATGCAGAAATATGAATAAGGTAGAATATCTGTC2290.11301275218129417No Hit
CTAAGCGGGATGAACTTTATGCTTCACTGTTAGTTGTTTGTGGAAGTTATTT2290.11301275218129417No Hit
CTACTTGGGCCGATCTAAGTAAGGGCTACCAGGCTTCGGAATAATCTCCCAT2280.11251924671325358No Hit
AGTTGCGGGCTACAGGGGGTATTATAACTTTGTTATGTGTTGTGTATGTGGC2280.11251924671325358No Hit
CAAAAAGGGATTTCTATGGGTGGGTCTAATTTGGTAACGGTTTGATTGTGCC2280.11251924671325358No Hit
GTTAGTGGTCAGAATCAGGAAAGTAAAGTTTTAAGGATTGACATGAAAGGTT2280.11251924671325358No Hit
ACTTCAGGGTTAATGACAGGCCTGCCAGAGCCTCTTTGTTGCTTTCAAAAAG2270.11202574124521299No Hit
CGCTCCGGGATGTGGACCAAGGTCAGGTTAGTTAATTTGTTTGCTTTTATTG2270.11202574124521299No Hit
TAGAATGGGACTTCTAGAGATTTTTGAGAATTGTAGCATCTTCCATGTGAAT2270.11202574124521299No Hit
CATATAGGGCGCTCTTCCGATCTTGTTGCAGGGGGTCTAACAGGTTAATTGT2260.11153223577717242No Hit
GTAGGCGGGCAGAGAGTTCCACTGAGAAGGCAGAAGAATAAGGTGCCCATAG2250.11103873030913183No Hit
TCTCACGGGCTTGCCACTTTGGGAGGCTGAAGTGTGAGGATTGCTGGAGGCC2250.11103873030913183No Hit
ATTGAGGGACAAGCGACATCCCAGATTATAATTCTCTGCTAAGATTTAAGTA2240.11054522484109124No Hit
ACTAAAGGGCTGGCCTACTGTTTTGGCTGGCATCTGGATGGACTCCAGCATT2240.11054522484109124No Hit
GAACGCGGGGGGCTTAGGTGATTCTCCCACCTCAGCCTCCCAAGTAGCTGGG2240.11054522484109124No Hit
GCACAAGGGCGATCTTGCACCGGGGCTTTTCTTCCTTTTGGTGCGAGCTTGC2230.11005171937305065No Hit
CTCTGTGGGTTTGGACCTTATTTTGAGGTTGTCTGTCTCGAAGAAAAAGAAA2230.11005171937305065No Hit
GTTAATGGGACCCCTGCCCAGCAGCCGGGCCGTGGTCTGGCTGAAGCTGCGG2230.11005171937305065No Hit
TTAGCGCGGGAACCACCACCACCGTTACTACGATCAATGTTTGCGCATGTTC2230.11005171937305065No Hit
CAATAAGGGATGGAAGGGGCCAGTTGCTGTTTTTTCATGCAGTGCCCTGGGA2220.10955821390501008No Hit
TGACCCATTACTCAATTGTCCCTGGCATATTATCTGATCTTCACGTTTCTTG2220.10955821390501008No Hit
AATTCAGGGATTTCTGGAATGCAGAAAACATTTTAAAGGCTAGATTTTTAGA2220.10955821390501008No Hit
TATGCAGGGGGTGAAATAATCTAGCCAAATATGTCACATATACATGTTCCAA2220.10955821390501008No Hit
TTGGGTGGGCATCTATGGGTTCTGGTAGTCCTGGGAGAGGCAGGGAAATGTC2210.10906470843696947No Hit
GTTTGTGGGATAGGCCTATATGCTCCATGAATATACAGAAATCCCATGCAAA2210.10906470843696947No Hit
AAACTTGGGCTTGATACATAATGCCAAATTATTCTTCATAAGAGCTGTTGCC2210.10906470843696947No Hit
TAACGAGGGTCCTCAGCAAAACTCTAAGCTCCTTGTGTTAAACATAATTTTT2210.10906470843696947No Hit
CAACAGGGGATTATTAGGCATTATATTTCTATATCAAAATATCTCATGTACC2210.10906470843696947No Hit
GTCAGGGGGGACTTTCGGGTCTTGTTTTTGTATATCCTTTATGAGAATAATA2200.1085712029689289No Hit
CTTTCCGGGCTCTGGAATTGTTGAATGTATTCTTTTATATAACTACATTAAA2200.1085712029689289No Hit
CACCAAGGGGCTCTTCCGATCTACTTGTGGGAGAACAAGCCATCGTCATCAT2190.10807769750088832No Hit
ATTGGTGGGAGGAAAAAGAAGATAAAGTAGGAGAGGGATCAGTTTCACATTC2190.10807769750088832No Hit
TTTTGGGGGATCAAAACAAAATGGAGGAAAACTAAGCATTGGCCTCATGTTC2190.10807769750088832No Hit
TTCGCCGGGAAGGTGTTTTGCAAGAGCTGTATTTATATTACAGTTTTTTAAA2180.10758419203284772No Hit
TGGCTCTGGGATATACTGACTAACCTATTGAATGTGATTCTCTTCTTCTTTG2170.10709068656480714No Hit
ATTGGCGGAGACGTTTTTTAAAATCCCGATATGAACAACCGAAGCCCTCCAC2170.10709068656480714No Hit
ATATCCGGGACTAGATTTCTTTCCCACTTGTTTTCTATGTCTCATGCAATTC2170.10709068656480714No Hit
CTCATTGGGATCCACATCTAGAAAGGGCTGCTCACTGCACCAGAAAGACAGT2170.10709068656480714No Hit
TCGAGAGGGATCTGACCTGGAATATAAAAATACTTTGTTAAGTAAGATACAT2170.10709068656480714No Hit
CTTACCGGGTACATCCGGAGCCAACCTGTGGCGCAAATCTATGTCCATGGGG2160.10659718109676657No Hit
TGATCCGGGATCCCTACTACATGGGGCTTATACTGTAGTTGTGAACCCAGGA2150.10610367562872595No Hit
TGGCCGGGGGAGGCAGAAATCTGCATGAGACTTGGCAAGGGTACTGGGATGT2150.10610367562872595No Hit
TACAAAGGGGATAGTTCTGACGGAGTCTGGCAGGTCTGCTCACCAGGTGGCC2140.10561017016068538No Hit
AAAAATGGGCTGTTATTCACATCTAATATTAATATGGTGTATTCGTTAGAGT2140.10561017016068538No Hit
GCTAAAGGGGAATGCTCAAGGCATTTTGCTTGTTGACTTTCTGTAGGGCCAA2130.1051166646926448No Hit
TTTCCGGGGCCTACCTTCCATACCAAGTCACTGTGCATCCTACATGTATTGA2130.1051166646926448No Hit
TATTTTGGGCGCTCTTTCGATCTTATTTTGGGAGCCACGGCGGGGGTGGCTG2130.1051166646926448No Hit
ACAGACGGGGATGATTATAGAGTTCCCCAAAATGGCCTTCTTACTATTTCTA2130.1051166646926448No Hit
AGTCGAGGGGGGCCCAGGCCGCCCCCGCCGTGGGGCCCAGGAGCCAGAAGCA2130.1051166646926448No Hit
CGTACGGGGATTATCACACACTTGGAAAATACAGAAAAGTGGAGGAAAAAAA2120.1046231592246042No Hit
ATACGAGGGATGCCCAGGCTGAGATGTCTAATAAAGCTAGTGACCATGGCTG2120.1046231592246042No Hit
GATTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2110.10412965375656362No Hit
CGCAATGGGATCCTCTGAAATCTAGGCAGAGGTTCCCAAACCTCAATTCTTG2110.10412965375656362No Hit
ATTTGAGGGAAGCTTTTACATTTTTTAGTAAAACTCAACTGATTTCTTGTCT2110.10412965375656362No Hit
GCTCTAGGGTATGAGCTCAGGAGTTCGAGACCACCCTGGGCAACATGGTGAA2110.10412965375656362No Hit
AAACCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2100.10363614828852305No Hit
GTATATGGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAG2100.10363614828852305No Hit
TTCTATGGGCAGTTTGGTCTGTTATTGATATAATTTAATGAGTTTTGTTTAT2090.10314264282048245No Hit
CAGTCGGGTACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAATG2090.10314264282048245No Hit
AGTTGTGGGTGGCATTCCTTGACTAAAAAGGACCACCTGATGCTCAGTCCCG2080.10264913735244187No Hit
TCAACTGGGCGACCGGAGCCGTCTTCCCAAATCGCCACCCTACACTGCTTTT2070.10215563188440129No Hit
GGACTTGGGAAATTCAGTTTATTAATAGCCAGGTATATCCTGTATATCTCCA2070.10215563188440129No Hit
GTCAAGGGTATGGAAGGGGATTTTGAGAATAGCAGTATTTGAGTAAAATTTA2070.10215563188440129No Hit
CTGTCAGGGCAACTGCTGCTTGCAAGTTTACTGGACAGAAACTGTTATTCAA2070.10215563188440129No Hit
TATGTAGGGCCAAGGCAGTTGGACCTTTGCTACTACCACTGCCCTGAAACTG2070.10215563188440129No Hit
GTCTAAGGGAGGCCAGGTCACCGACAAAGGAAAGCTCATTAGACTAACAGCA2060.10166212641636069No Hit
ACCTGTGGGGGCATTTTGCTTGTTGACTTTCTGTAGGGCCAAAGAATGGTAA2050.1011686209483201No Hit
TGCAGAGGGAGAAAGACCTTGGGCTTTGGGCTTAAATTGCACCTGCAGCATT2050.1011686209483201No Hit
TGTCCAGGGGAAACTGAGGCAGGATTTCCTGCCTCTAAACTATTGGCAGCAA2050.1011686209483201No Hit
AGCTTAGGGGATTTCTGATTAAAAACAGAGTTGGTTTTTATCCCTTATCTTG2050.1011686209483201No Hit
ATCACAGGGTATGACCCCAACCTCAGGTGGCAGCACCCCTCCGGGCATCACT2050.1011686209483201No Hit
TTACACGGGTAAAAGTCCTATGTTTGCCAGACCCTAATAAAGCTACAAGGTT2040.10067511548027953No Hit
GTAAAAGGGAATCCAAATGGGACAATCTGATAAGAATTTCATGCATTGGTAG2040.10067511548027953No Hit
CTCGTGGGGAGGGACCAGCTACAGAAGCAAATCCAGCTGATGCAGAAGCCAG2040.10067511548027953No Hit
TTACGAGGGTAGTGGTCATTTGGTGGCTCTAACTCCTTGAGGTCTTGAGTTT2040.10067511548027953No Hit
TAGTTAGGGACTAAATGAAGAATAAACTGTTTAATATCTGAATATAATTACC2030.10018161001223894No Hit
GTATACGGATTATGGTTTTGGCTCACATTTCTCTAGCGATGTTGGGCATCTT2030.10018161001223894No Hit
TTTTCTGGGGTTATTCCCCCCTACTTGAGATAATCTAAATATAAACCAGCTA2030.10018161001223894No Hit
TAGCTAGGGATAGAGAGTATGGCCAAGACCATACTTAATAAATGTCATGGAA2030.10018161001223894No Hit
CGACAGGGGCTGGAAGCTGAGCACACACAGGTGCACAGCCATGCTGTGGTCT2030.10018161001223894No Hit
TGTTCGGGGGGGAGTCATTCAGAAGTGATAGCATTTTATTTGTTTGAATACT2030.10018161001223894No Hit
GAATCAGGGCAAGGTTTGTAACTTAGAGGAATTCTTTGATTCTGTGACAAGG2030.10018161001223894No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)