FastQCFastQC Report
Sat 31 Dec 2022
SRR5847317.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847317.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199176
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT8370.42023135317508137RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC7530.3780575973008796No Hit
GTCTGGGGCAGAAGTGAAAGATGAGAAGCTTAATGTAGAGTAAAATGTCTGA3100.1556412419166968No Hit
AAGCGAGGGGTGTGCATGACTTTAATGACTTTTTTAATGGGGTGGGAGGTGG2980.14961641964895367No Hit
TTTAGTGGGGGCGTCCCGCAGGAGCCGCAGGCAGAGCGTCCGGCGGCCGGGA2970.14911435112664176No Hit
TGGCCAGGAATGCTGCGGGACAGTCTACTTTGATTGCTTTCTTTCCTCCATG2930.14710607703739406No Hit
ATCGCGGGGATTGGAGTAGCGAGGAATCTGATTCCAAGCAAAAACCAGGCTC2800.14057918624733903No Hit
TAGACTGGGATCATCTTATAAGTGAGCACCTTCCCTGAGACTCTTTAACTTC2770.13907298068040327No Hit
AGTTTAGGGGCAGAAAGTCTCCAGACTGAAGGGTTTGAACATCACCTTATAA2760.13857091215809134No Hit
TATCCGCGGGTGGCTCACTGCAACCTCTGCCTCCCGGGTTCAAGTGATTCTT2760.13857091215809134No Hit
TATGTTTGGGGAAACAAAACAAAACAAAACAAATAAACAAACAAACACGTTC2740.1375667751134675No Hit
TTCTATGGGATTGGCTCCTTCGTACTTCTTTTACCACCTCTATTTATTAATT2730.13706470659115555No Hit
TTCTACGGGAAAAATTAAGGAAGTATCTTAGTAAAATAAGAAGACAAAGTCA2710.1360605695465317No Hit
CTGTCGGGGAGGGGAATATGTCACATTAAATACAAAGGGAATTTTTGTAGGT2700.1355585010242198No Hit
CTACTTGGGCCGATCTAAGTAAGGGCTACCAGGCTTCGGAATAATCTCCCAT2690.13505643250190785No Hit
TTTCAGGGGCAGGTCAAGCATTTAAACCCTGAAACAAAAGTCAGAACAAACT2680.13455436397959594No Hit
GTTTACGGGCACCTTCTGTCCAGGTCATTTTGTTCTTTACATGTATCCATGG2660.1335502269349721No Hit
TAGTAAGGGGTGGGTAAAAAAATGAAAGCATTTTGGCTGGTTAACAAATCAA2660.1335502269349721No Hit
TTTTCAGGGAACTTTATAAACATAATTCAACCACATGGGAATTCTGAGTAAA2650.13304815841266016No Hit
TCATATGGGACCTTTCTCAGAGGTATTTGCATTTTAAATGAGGGTGGCTCTC2610.13103988432341246No Hit
GTGCTCGGGCAAATGGCCTAGACATGGTTAATGCAATTTGTTGCTAGTGGAA2610.13103988432341246No Hit
TTGCTGGGGTGCCCGAGAAAATGAAATGGATGAAAACCTAGAGCAGGTGAGC2600.13053781580110055No Hit
GGCATGGGGTACCATGAGGAGCAACAGTGATAATTTATTTACTTATTTATTT2580.1295336787564767No Hit
AATCGCGGGATAATCTCATTTTGGTTCACTGAGGAAAACCCTGCTTCACAAG2570.12903161023416476No Hit
AACAACGGGAACTAATTAATATTAAAGACAGAATTGAAAGATACCTTGTTAT2570.12903161023416476No Hit
TCATACGAGGGGCGCCGCTGTGGGAGAGACCCCAGCAGGAGCCCAAAGGCAG2570.12903161023416476No Hit
GGCTCTGGGCGGTTATGTTGTGTAAGTCAACTCTTCAGCCTCATTCACTGTC2560.12852954171185285No Hit
TCAACCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2560.12852954171185285No Hit
TGATCAGGGCAGCTGGTTATTGGTAGAGCTGTCACCCCCTAGTTCTGACTTC2550.1280274731895409No Hit
ACTTCTGGGAGCTCATAGTATGCAGAAATATGAATAAGGTAGAATATCTGTC2530.12702333614491707No Hit
TAGTGCGGGATCATCAGTCATTACGACACAAAAACCAAAAGCAGAATGAGAC2520.12652126762260515No Hit
ACTGCTGGGAAACTCATAATCTTCTGAGATTACAACTTACGACTCTTCTTTG2520.12652126762260515No Hit
TCGGCCGGGTCTGCTTCCGGGGGAATCTGTAACATAATAAATTTTTTGTCTT2510.1260191991002932No Hit
CATTGTGGGTGTAAGGGCAGACCTGCATCTGTGTGTGTGTTTTCAGTGGGTG2510.1260191991002932No Hit
CAAAAAGGGATTTCTATGGGTGGGTCTAATTTGGTAACGGTTTGATTGTGCC2510.1260191991002932No Hit
GACTTTGGGGCAAGGACCCCGGTTTCTTCGTCGGAGGTCCGCGCAGGGCTCT2490.12501506205566934No Hit
GCATTTGGGACATCCCAGACATTAGGAGTTCATAAGTGTATTTAATGAAATT2480.12451299353335743No Hit
AGTTGTGGGTGGCATTCCTTGACTAAAAAGGACCACCTGATGCTCAGTCCCG2480.12451299353335743No Hit
TCTCACGGGCTTGCCACTTTGGGAGGCTGAAGTGTGAGGATTGCTGGAGGCC2480.12451299353335743No Hit
GATTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2470.1240109250110455No Hit
CTATATGGGCAGGGGTTTGAAGCACACAGGAGCTGGACATGTCAATTCTGTA2470.1240109250110455No Hit
AGTTGCGGGCTACAGGGGGTATTATAACTTTGTTATGTGTTGTGTATGTGGC2460.12350885648873358No Hit
AACAACGGGGCTACTGGTTTTCTTCCTTCTACTCTGCCTACTATCTTCTTTC2430.12200265092179781No Hit
TTCAGTGGGATGCTGGTAGGGCAGGGGTGAAATATAAACAAGTAAATACACA2430.12200265092179781No Hit
GCTTGGGGGGAGAAATGAGTTGGAAAGCTATGCCTATTCTCTAAAGAATCAG2420.12150058239948588No Hit
TCTCAAGGGGGCAGCTTTTAGGCTGCAGAGTGATGAGCCTCGAAGCAGAATT2420.12150058239948588No Hit
AACGGCGGGCTGATGAACACTAAATATTAATTTCAATTAGCTAGATTGTACA2410.12099851387717396No Hit
GTAGGCGGGCAGAGAGTTCCACTGAGAAGGCAGAAGAATAAGGTGCCCATAG2400.12049644535486204No Hit
ACTTGTGGGCTTGGATAGATCCTAAAAGAAACAAAAAAATTACCTTTGAAGA2380.11949230831023819No Hit
CTCATTGGGATCCACATCTAGAAAGGGCTGCTCACTGCACCAGAAAGACAGT2380.11949230831023819No Hit
ACACCTGGGGAGTAGTCAAATTCATAGAGACAGAAAGTAGAACGGTGATTGC2360.11848817126561434No Hit
CAATCGGGATTCTTAAAAATTGAAAACATAAGCTAAAGAGATGACTAAATGG2350.11798610274330242No Hit
CAATAAGGGATGGAAGGGGCCAGTTGCTGTTTTTTCATGCAGTGCCCTGGGA2340.11748403422099049No Hit
TAGAATGGGACTTCTAGAGATTTTTGAGAATTGTAGCATCTTCCATGTGAAT2330.11698196569867857No Hit
GCAAGTGGGGGGGAAAAGGCTCTTAGAGCTTTTTAGTGGACTGACACATAAT2330.11698196569867857No Hit
TAAAATGGGGAATTGGAATCAGTGAGTTAAAAATTATGGACATGTCATACAT2320.11647989717636664No Hit
CTCGCGGGGGTGGAAGTCCTAGCTAGGGCAATCAGACAAGAGAAAGAAATAA2320.11647989717636664No Hit
GAATCTGGGGGCTTGTAAAATGTGACATGTTAACATTTTTCTCCTTAACCTC2320.11647989717636664No Hit
ATAAAGGGGTGGCGTTGAAGAGTCATCTACTTTTCTAGCTAACTCCTGTGCT2320.11647989717636664No Hit
TTCACTGGGAGGATATTTTGAACCAATAGAATTCTAGGTTGGCAATTATTTT2320.11647989717636664No Hit
ATAGCTGGGATTGTAGGCGTGCGCCACCACGCCCGGCTAATTTTTGTGTTTT2310.11597782865405472No Hit
CTAAGCGGGATGAACTTTATGCTTCACTGTTAGTTGTTTGTGGAAGTTATTT2310.11597782865405472No Hit
TTGGGTGGGCATCTATGGGTTCTGGTAGTCCTGGGAGAGGCAGGGAAATGTC2300.1154757601317428No Hit
GCACAAGGGCGATCTTGCACCGGGGCTTTTCTTCCTTTTGGTGCGAGCTTGC2290.11497369160943087No Hit
TGTAACGGGAGATTCTCGGATTCTCGACTCTTTGCAACAGCACTTGCTTGCG2290.11497369160943087No Hit
TGCTGTGGGGAGAGTGAGGTTCAGGAAAGCAGCCTAAGCTGAAAATATTGTT2280.11447162308711895No Hit
GTTTGTGGGATAGGCCTATATGCTCCATGAATATACAGAAATCCCATGCAAA2280.11447162308711895No Hit
ATATCCGGGACTAGATTTCTTTCCCACTTGTTTTCTATGTCTCATGCAATTC2280.11447162308711895No Hit
TGTTTAGGGTTATTGCCATTTTACAGATGAAGGGACCAAAGCTTGAAGAGTT2280.11447162308711895No Hit
CACCAAGGGGCTCTTCCGATCTACTTGTGGGAGAACAAGCCATCGTCATCAT2270.113969554564807No Hit
ATTGGCGGAGACGTTTTTTAAAATCCCGATATGAACAACCGAAGCCCTCCAC2270.113969554564807No Hit
GAGTGAGGGGCTGTCTATTTTTCATGTTATCAGTTTCTTTTGGTGCCTAGTT2270.113969554564807No Hit
AAGCCTGGGGAAGAGCATCTTGGGCCCTGAGTTAATTTAATAGATCATGGAG2270.113969554564807No Hit
CAGCACGGGCACAGGCTCTGCTTCCCTGCCACCTTGGGTAGCTCCTGCCTTT2270.113969554564807No Hit
ACCGCGGGGGAATAGACTTCCTAGCTTATTCAGAGTGTTGATAAAACTTTGT2270.113969554564807No Hit
TGATCCGGGATCCCTACTACATGGGGCTTATACTGTAGTTGTGAACCCAGGA2260.11346748604249507No Hit
AACGATGGGGGAAACCAAAACACCTACCCTGCAACCAACTCCTGAAGTTCAC2250.11296541752018315No Hit
CAATACGGGGTTCTACTTTAATTCCTTTTTTAGGGAACTACAACCATCAAAT2240.11246334899787122No Hit
GAAATAGGGACAGGCTAGGTTCCTTGTAGCATGTGGGGGTTTCTAGAGGGGA2240.11246334899787122No Hit
CGTACGGGGATTATCACACACTTGGAAAATACAGAAAAGTGGAGGAAAAAAA2230.1119612804755593No Hit
CGACAGGGGCTGGAAGCTGAGCACACACAGGTGCACAGCCATGCTGTGGTCT2230.1119612804755593No Hit
ACTAAAGGGCTGGCCTACTGTTTTGGCTGGCATCTGGATGGACTCCAGCATT2220.11145921195324737No Hit
ATAGGAGGGTGGTGTGAGATGGTATCTCATTGTGGTTTTGATTTGCATTTCT2210.11095714343093545No Hit
GCACTAGGGGGAGCCAAATAACACATGTTATAAACAGGAAATATCTTCCTTT2200.11045507490862352No Hit
TTATACGGGAGGCAGACAAATTTGGCTTCAGATCCTGTTCCTCTGATTAGCT2190.1099530063863116No Hit
CGAATAGGGAATCCTGGTGGAAAAGCCACAGAACCTTCTTCCTGCCCTTCTT2190.1099530063863116No Hit
CTGTCAGGGACCCCTAGTCCTTTTAGGGTACCTATTTCTGTGTGCTAGTTCT2180.10945093786399968No Hit
TGATAAGGGGTTAGATAAATATCTCAAAGTGAGATTTCTGAGCAGAGTGTAT2170.10894886934168775No Hit
TCCAGCAAGGGATGGCCCACAGCTCCTGTGCTGGCCAGACTCCCAGAAAATT2160.10844680081937583No Hit
GTTGCCGGGATTGCTTAATCTTTTATATGGAATTTCACTTTATAAAACAAAG2160.10844680081937583No Hit
GACGCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2160.10844680081937583No Hit
TTCAATGGGATGTAGACTGTACCCCTAAAGCACACATCTTTGTCTTTGAATA2160.10844680081937583No Hit
ACTTCAGGGTTAATGACAGGCCTGCCAGAGCCTCTTTGTTGCTTTCAAAAAG2150.1079447322970639No Hit
GCACATGGGATGCCTCTGATCCTTTGCTCTGAAAACAAAGCCTAAAACAACT2140.10744266377475198No Hit
ACCTAAGGGAGACTTTGTCAAATGGACTGGTCCTGGCAGATTCATAGCCACA2130.10694059525244005No Hit
CTTTCCGGGCTCTGGAATTGTTGAATGTATTCTTTTATATAACTACATTAAA2130.10694059525244005No Hit
GTCTGCGGGATTGTAACAGACATTGTTTTGCCAACATTGCCTATTTCAGTGG2130.10694059525244005No Hit
TGGCCGGGGGAGGCAGAAATCTGCATGAGACTTGGCAAGGGTACTGGGATGT2130.10694059525244005No Hit
GATTCAGGGAGTTGGACTGTGTTACCATTTTACTGATGAAGAGGCTGAAAGA2130.10694059525244005No Hit
GTATACGGATTATGGTTTTGGCTCACATTTCTCTAGCGATGTTGGGCATCTT2120.10643852673012813No Hit
CGGTTTGGGAGAGAAGGGGTTCTATAACTGCTTTTGTTTTGTTTTGGATAAA2120.10643852673012813No Hit
AAACTTGGGCTTGATACATAATGCCAAATTATTCTTCATAAGAGCTGTTGCC2120.10643852673012813No Hit
GACACTGGGATTCTTGAATTTCTTGTTACATAGTATGTGAACTAAGGAATCT2120.10643852673012813No Hit
CAACAGGGGATTATTAGGCATTATATTTCTATATCAAAATATCTCATGTACC2120.10643852673012813No Hit
TATTTTGGGCGCTCTTTCGATCTTATTTTGGGAGCCACGGCGGGGGTGGCTG2110.1059364582078162No Hit
ACAGACGGGGATGATTATAGAGTTCCCCAAAATGGCCTTCTTACTATTTCTA2110.1059364582078162No Hit
CATCGTGGATCCAGCTTTTTCTTGTCATTTTAATAAGAGTATGTGTCTTATA2110.1059364582078162No Hit
GTTAATGGGACCCCTGCCCAGCAGCCGGGCCGTGGTCTGGCTGAAGCTGCGG2110.1059364582078162No Hit
GAATTAGGGCTGCTGCTTAGATAATGTCACTGTTAGCATAATGATATATGTT2110.1059364582078162No Hit
ATTGAGGGACAAGCGACATCCCAGATTATAATTCTCTGCTAAGATTTAAGTA2100.10543438968550428No Hit
TAGATGGGGTGCTGGTCAGTGGGACATTTTGTCAACTATGGGTATTGGGTGC2100.10543438968550428No Hit
GACTGGGGTCAGAAGCCATATGCAGAGGCTGTGGAACAAGGAAGTGGTGAGG2090.10493232116319236No Hit
AAAAATGGGCTGTTATTCACATCTAATATTAATATGGTGTATTCGTTAGAGT2090.10493232116319236No Hit
TTGCCGGGCCTGCTTATCTTTGTTTGACATTCTGCAATTTATCAATAATTTG2090.10493232116319236No Hit
GACTATGGGTCCGCTGAGGGTTCCACCTAAGGGTGACCCCAAACGAAGGGAG2080.10443025264088043No Hit
CTTATAGGGGACGCTCTTCCGATCTGTGGGAGGGTTGCTTGAGCCCGGGAGG2070.10392818411856851No Hit
AAGTGGGCGTGTGGTGTGGTATGTAGAAACTTAATGTACACTAGTGTTTCCT2070.10392818411856851No Hit
TTCGCCGGGAAGGTGTTTTGCAAGAGCTGTATTTATATTACAGTTTTTTAAA2070.10392818411856851No Hit
TTAGCGGGATAACATTTCTTCTTTCAACAGAGTATATGTTTCCCATTTAACC2070.10392818411856851No Hit
GCAATGGGGTAAGTCTCCAGTCACCTTAGAGCTGAAGTCTCACTCTGACAAC2060.10342611559625658No Hit
CACTGCGGGAACCTTGCCTCAGAAAAATGTATGTACACACATATATACAACT2050.10292404707394466No Hit
GTATATGGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAG2050.10292404707394466No Hit
CAAGTGGGGATGTCCAATGCCGATTTTGCCAAGCTGTTTCTGAGAAAGTGAA2050.10292404707394466No Hit
GTTTGAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2050.10292404707394466No Hit
ACCCTGGGAATATATGACATGACATCTGTGTTACCTTCAGCAAGTGCGTCCA2050.10292404707394466No Hit
GCTTATGGATTATCTGCCTCATTTATGGTATGATGGCATTCTGAGTCATATC2040.10242197855163274No Hit
GTTAGTGGTCAGAATCAGGAAAGTAAAGTTTTAAGGATTGACATGAAAGGTT2040.10242197855163274No Hit
CTGTCAGGGCAACTGCTGCTTGCAAGTTTACTGGACAGAAACTGTTATTCAA2040.10242197855163274No Hit
ATTTATGGGATGGGGACTCATTTTATGATGGACAACTTGAGAAAATTGAGTT2040.10242197855163274No Hit
AATACTGGGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTT2040.10242197855163274No Hit
ACCTGTGGGGGCATTTTGCTTGTTGACTTTCTGTAGGGCCAAAGAATGGTAA2030.1019199100293208No Hit
AATAGCGGGATGACTGTAATTGCAGTGTGTAAACTACTCATATCTTCAATAG2030.1019199100293208No Hit
CTTACCGGGTACATCCGGAGCCAACCTGTGGCGCAAATCTATGTCCATGGGG2030.1019199100293208No Hit
TGAATAGGGAGGTGGGGTGATTGAATAAATTCTACTAATCTCCATTTCACTC2020.10141784150700886No Hit
TAGCGCGGACGAGGCTCCTGCTTACCATCTTATTTTGGAAGGGATTCTGATC2020.10141784150700886No Hit
AACTTAGGGAGGTTGACATATGGAGAAAAATCAGACCATATGGGATTATGAA2010.10091577298469695No Hit
TCGAGAGGGATCTGACCTGGAATATAAAAATACTTTGTTAAGTAAGATACAT2010.10091577298469695No Hit
TGCTCGGGATTATAGGAAATTATAGGAGATTTATTCAGTTTCTTCATGGCTG2000.10041370446238501No Hit
TTCTATGGGCAGTTTGGTCTGTTATTGATATAATTTAATGAGTTTTGTTTAT2000.10041370446238501No Hit
GCTCATGGGTCATTTTTTCCCTGATGTAGATAAAATTCCATCAGCTAAGTTC2000.10041370446238501No Hit
GAGAAAGGGACTGCAACAGTATCTTTTTGTCAATGTTCCACATAATGCAAAT2000.10041370446238501No Hit
TGTCGTTGGGCAACTGGTAGTCCATTAAATTCTCCTGTCTAGAATGACCCCC2000.10041370446238501No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)