FastQCFastQC Report
Sat 31 Dec 2022
SRR5847307.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847307.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131930
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT3550.2690820889865838RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC2870.21753960433563255No Hit
GACAACGGGAGTAAAGCTTTGTGCAATGATCGAGTCATCAACAGTATTCCTT2050.15538543166830895No Hit
AAAGCAGGGGTGATTGCTCTGGTGAGACTGAATGGACTTGCTGGTGAAATGA1900.14401576593648147No Hit
TGCTGGGGGTGTGGGACCGGCGCCATCGCTGAGCGAGCTGGATCTGCGGCCG1880.14249981050557115No Hit
AGAAAGGGGAAACCAAGGGCGAGGGACCAAAGAGAAAGAAGTGACAGAGTAT1770.13416205563556433No Hit
TGCTGCTGGGACGAAAATTAATGTTTCAAAGAGCTGCAAGGAACTTTATATG1750.132646100204654No Hit
GGTTCTGGGTTCACCTCCCTGCTCATGGAACGTCTCTCAGTTGATTATGGCA1720.1303721670582885No Hit
CAGGCGGGGGGGGCTCGCGCCGCAGAGGCCGGTGAGGCGCCGGCGGCCACGC1720.1303721670582885No Hit
TAACTTGGGGATAGCAGATGCTTTTCATTTTTAAACATAGAATTAAAATTGG1680.12734025619646783No Hit
AACGCAGGGGCGAGAAGTGGGACGCACCAAACTGGAGGTCAAGGTGGTCATC1630.123550367619192No Hit
TAGATAGGGTGTCAGGATGCCTTTGTTAGGATCTGTATTTGCCCTTAATTTT1620.12279238990373684No Hit
AGACGAGGGCCCTCCTCCTACAAGGTTTTGAGTTGGCTGCTGGTTAGCAAAC1620.12279238990373684No Hit
TATAGTGGGAGTGGTATAAAAGACACGAATATCTCCTGGTCTATAAGGATAC1610.12203441218828168No Hit
ACGCTGGGACTGCTGGCAGGAGAAGGCCCATTTTCACAGTCCACAGAAGCGT1610.12203441218828168No Hit
TTTACTGGGAGTCCCACAGGTCCCTAAAGCTCTGTCCATCTTGTTTTCCCAT1600.12127643447282649No Hit
GCTGCTGGGGTGCCATTTTGTCATAGAATGTAAAAATTGGTTAACTTTACAA1580.11976047904191617No Hit
AGACTGGGGGTGGAGTTTGCCATCCAGATTTGTTCCATATTAGTTTATCCTT1570.11900250132646101No Hit
GCTCAAGGGTGGATGAAGAGAAGAAAATCCGGCTTCGGTTGCAGATGGAAGT1570.11900250132646101No Hit
TTCGCAGGGCTACCTGGGCAGTGCCAACATGGACTGGCGTTCACTGACCCAG1540.1167285681800955No Hit
TTCTACGGGAGGTTTTCTAAATGTGGTAGGAAAATGTACTCTGGAAAAATGC1540.1167285681800955No Hit
AGCGAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGA1540.1167285681800955No Hit
ACTAACGGGTCTGCAGGGGCTGAGCTATAATTTGTTTTTAATGCAAAGACAC1530.11597059046464034No Hit
TACACAGGGATGCCCACAGATTTCTTGCATGTTTCCAGTTGTATTTGATCTC1530.11597059046464034No Hit
CGTTAAGGGGGAGGTGGGGTTGATGGGTTTCCTTATCCCCAAAGTCACTCCT1520.11521261274918518No Hit
TGCACGTAGGGGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTAT1500.11369665731827483No Hit
GACTTGGGGATTACTTATCAGGCTTAAGAGTCTGTTCTATCAATCTTAAGGT1490.11293867960281967No Hit
CTATGCAAGGGAGTGTGGCTGCTGGAGGACGCTATGATGGGCTAGTGGGCAT1490.11293867960281967No Hit
TTTGCAGGGAAGAAGAAAGCCTGGCCCAAGATGTGAATAAGAGTGTAATGAT1480.11218070188736451No Hit
ATTTAAGGGGACCGCGAAGGGAGGAGTGGCAACATGGCGTCTTCGGGAGCTG1470.11142272417190935No Hit
GATCTTGGGATGATGTATGCAGGATCCCAGCATTGATACCCAATGACAAACT1470.11142272417190935No Hit
AACCTAGGGGACCTGTCCTGCAGCACTGGATGCTTTGTGAGTTGGGGATTGT1460.11066474645645419No Hit
AAACTCGGGGCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAAC1460.11066474645645419No Hit
TCAAGCCGGGGGTTTCAAGGACATTTTGGCTGTTAAGATTCCTTTTCCTTAT1450.109906768740999No Hit
TACTCAGGGCTAAGGTAACTAGGCCCCCTCAAGAAAAAGTGGCATAGGGGAA1450.109906768740999No Hit
ACTTAAGGGAATAATGGAAACACAACATACCAAAACCTATGGGATACAGTGA1450.109906768740999No Hit
GTCGATGGGGATGTAAACACCCGAGCCGGGCTCCAAGGCCCGGGAGGTCAGA1450.109906768740999No Hit
TAAGTAGGGGAACTTTTGGAAATTAATTTATACATAATGAAAGAACAAACTC1430.10839081331008868No Hit
AATGGGATTGTTTTAAAGTAAACCAGATCTCCAAATACCTATAAAACAAACC1430.10839081331008868No Hit
CATTCCGGGAATGCCCAAGCTGATTCCCCCACTGAAAGCCCTGTTTGCTGGC1430.10839081331008868No Hit
CATCAGGGGATGTATCTTTAACACTTTTGAATAGAACAAACAGCTTTTCCAT1420.10763283559463352No Hit
ATTGAAGGGCGATCTATGCATGGGGCATCAGGGATAAGGAAAGGTTAAAAAA1420.10763283559463352No Hit
GACACCGGGAACCAGAAAATTAATTAATCTCTAAAATTAAACTTTACACTGA1410.10687485787917836No Hit
GTAGAGGGGAGTCAAGATGGCGGGAGCAGCTACCCAGGCTTCCCTGGAGTCG1410.10687485787917836No Hit
AAATTGGGGCGATCTTTACTGGGGGCCGGACAGAGGATCGCCTCGCTGGCCT1410.10687485787917836No Hit
CAGTACGGGGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAA1410.10687485787917836No Hit
TCTGCAAGGGGAGAGAAAAATGGGGAGCATGAAAACAGAAAATTAGGGAGAA1400.10611688016372317No Hit
TATAAAGGGGGAAGGTTCGGGAGGCTTCTGGAAAAAGCGCCGCGCGCTGGGC1400.10611688016372317No Hit
TCTGCTGGGATCAGCTGAGAGATTATTTGCTGCTGTTATTCAAAAGGCCATT1400.10611688016372317No Hit
CGTCAGGGCCCGCAGGAGCTGACATGGACCCAAATCCTCGGGCCGCCCTGGA1390.10535890244826801No Hit
AAACTTGGGGTAGTTATCTGAAAATAAAAGGAGAGTGCTGAAGTTGAATATA1390.10535890244826801No Hit
GTGCACGGTGGTGGTTTGCACCAGGACAGTTGCTGTAGAGGAGGTGAACAAA1390.10535890244826801No Hit
TGTAAAGGGGTTTCTAAACAATAGCCATTTTATCGAATAGTTTAGAGACCAC1390.10535890244826801No Hit
TCAGGTTGGGACACTTAGAATACTACAGCATAAATCTGTTAGCATTATTGCC1390.10535890244826801No Hit
CATGTCGGGACTGCCATCACAGTCCTCATCGTGATGCAAGCTTATATGATTC1380.10460092473281285No Hit
AATAATGGGGATCTGTGCGCGGGGTACTATCCCTATGAGGCATAATTATAAC1370.10384294701735769No Hit
TTTGGCGGGGAAGGAAATTTACCTGTTCGAGGCACAATTCCTGATATGATTG1370.10384294701735769No Hit
TGTTACGGGCGCATTTTCCCATGGTGTATGACTAGTTCATCTCTTTCTTGAG1370.10384294701735769No Hit
TGAATCGGGTAGCACCATCACCCTTGAAAACTCTCAGTCAGTGTCATGAATG1360.10308496930190253No Hit
ATTGCAGGGTTATGCTTGTGGTCCCAGCATTTTGGGAGGCTGAGGTGGAAGA1360.10308496930190253No Hit
ACGTTAGGGCGCTCAGGCTGACTCTCGGGGGCGAGGTCGAGCCAGGGGCGGC1360.10308496930190253No Hit
GCCACCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGT1360.10308496930190253No Hit
CGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCA1360.10308496930190253No Hit
GTAGGTGGGGGCAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAG1350.10232699158644736No Hit
TAGTAGGGGGGGGGCAATAATGTTGTAGAACAAGTATCTGGTGAGTACTGAT1350.10232699158644736No Hit
TCTCAAGGGATAGTTCTCTCAATGTAAACTTGGAACCTGAAACCCTACTTAA1350.10232699158644736No Hit
GTAGGTGGGACGGCGGGGTAATGATGAAGCTTATGTTTATCATTTAAAAGAA1350.10232699158644736No Hit
GGAGTTGGGCTTTGTTTTATATAATGCATTCTGCATTTTTAAGAAGTGTTCA1350.10232699158644736No Hit
TAGCTTGGGAGGTGCTGTGTCATTCTCTGACAGCAGCTGCCTGGGCCCAAGG1340.1015690138709922No Hit
ATTATGGGGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGC1340.1015690138709922No Hit
ACAGTAGGGTCTCCTTCCTCATATCCAGTCAGTCATCAAGTCCTGTCTGTTT1340.1015690138709922No Hit
TATCAGGGATGGCACAAGGAACTGAAAGTAACTATTGGCTGGCTTAGGTGAG1340.1015690138709922No Hit
AAATCCGGGTCTGGAACCGGGTATTACTTTAAAGCCATACCATATATAAATC1340.1015690138709922No Hit
TAAGATGGGGCAACTTCAAATCGATATCAACTTCAACTAAAATGGTTAATGG1330.10081103615553702No Hit
TGAACCGGGCAGAGAAATGAACACATAATCATGCAAAAACCTGTACACCAAT1330.10081103615553702No Hit
TTACGACGGGGGCCAAGAACAAAAGAAAGATGCTTAATCTCACCACTTTTTT1330.10081103615553702No Hit
ACCAATGGGATTCTCTTCTTGCTATATGATTTAGCAAGTTCTAGAAGGTCTC1330.10081103615553702No Hit
GCATCCGGGGTCCTGTGGGTGCTGCTGGGCACCCCTGCAGGCTATGTTGCTG1320.10005305844008186No Hit
ACTCCGGGGAACGTGCTGGGCATCATCCCCTATGCGGGCATCGACCTGGCCG1320.10005305844008186No Hit
GGCATTGGACTTTTTTTAACTTGTTTTCATCTGTTTATCATGACTTTTTTAT1320.10005305844008186No Hit
TTGTAGAGGGGGCCTTGCAAATAATAGCAAGATATGTACCATCATGAGTATA1320.10005305844008186No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)