FastQCFastQC Report
Sat 31 Dec 2022
SRR5847306.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847306.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131361
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT3390.2580674629456231RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC2990.22761702483994486No Hit
TGCTGGGGGTGTGGGACCGGCGCCATCGCTGAGCGAGCTGGATCTGCGGCCG1820.13854949338083603No Hit
AGAAAGGGGAAACCAAGGGCGAGGGACCAAAGAGAAAGAAGTGACAGAGTAT1780.1355044495702682No Hit
CAGGCGGGGGGGGCTCGCGCCGCAGAGGCCGGTGAGGCGCCGGCGGCCACGC1760.13398192766498426No Hit
TTCGCAGGGCTACCTGGGCAGTGCCAACATGGACTGGCGTTCACTGACCCAG1750.1332206667123423No Hit
AAATTGGGGCGATCTTTACTGGGGGCCGGACAGAGGATCGCCTCGCTGGCCT1750.1332206667123423No Hit
GACAACGGGAGTAAAGCTTTGTGCAATGATCGAGTCATCAACAGTATTCCTT1740.13245940575970036No Hit
TAACTTGGGGATAGCAGATGCTTTTCATTTTTAAACATAGAATTAAAATTGG1730.1316981448070584No Hit
ACTAACGGGTCTGCAGGGGCTGAGCTATAATTTGTTTTTAATGCAAAGACAC1710.13017562290177448No Hit
TATAGTGGGAGTGGTATAAAAGACACGAATATCTCCTGGTCTATAAGGATAC1670.12713057909120667No Hit
AGACTGGGGGTGGAGTTTGCCATCCAGATTTGTTCCATATTAGTTTATCCTT1660.12636931813856472No Hit
GCTGCTGGGGTGCCATTTTGTCATAGAATGTAAAAATTGGTTAACTTTACAA1650.12560805718592277No Hit
TGCTGCTGGGACGAAAATTAATGTTTCAAAGAGCTGCAAGGAACTTTATATG1650.12560805718592277No Hit
TTTGCAGGGAAGAAGAAAGCCTGGCCCAAGATGTGAATAAGAGTGTAATGAT1630.12408553528063884No Hit
AACGCAGGGGCGAGAAGTGGGACGCACCAAACTGGAGGTCAAGGTGGTCATC1630.12408553528063884No Hit
TGAATCGGGTAGCACCATCACCCTTGAAAACTCTCAGTCAGTGTCATGAATG1620.12332427432799689No Hit
GGTTCTGGGTTCACCTCCCTGCTCATGGAACGTCTCTCAGTTGATTATGGCA1610.12256301337535494No Hit
GGGCAAGGGGACCCGTGCTGAGGCGGCGGCGGCGACGTGGGCTGCGGCGGGC1600.12180175242271299No Hit
GACTTGGGGATTACTTATCAGGCTTAAGAGTCTGTTCTATCAATCTTAAGGT1590.12104049147007104No Hit
CAGTACGGGGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAA1580.12027923051742906No Hit
AAAGCAGGGGTGATTGCTCTGGTGAGACTGAATGGACTTGCTGGTGAAATGA1550.1179954476595032No Hit
TCGACCGGGTGCCAAAGCCATGCAGGTAGTAGGCGGTAGATCTAACTTTTGT1550.1179954476595032No Hit
TACTCAGGGCTAAGGTAACTAGGCCCCCTCAAGAAAAAGTGGCATAGGGGAA1540.11723418670686124No Hit
ACTTAAGGGAATAATGGAAACACAACATACCAAAACCTATGGGATACAGTGA1540.11723418670686124No Hit
AAACTCGGGGCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAAC1540.11723418670686124No Hit
ACTCCGGGGAACGTGCTGGGCATCATCCCCTATGCGGGCATCGACCTGGCCG1530.11647292575421929No Hit
TATACGGGGCTGATAACCAGTGTAAGCTGGAGCTACAGGGCGTCAAGGGTGG1530.11647292575421929No Hit
TACACAGGGATGCCCACAGATTTCTTGCATGTTTCCAGTTGTATTTGATCTC1530.11647292575421929No Hit
CGTTAAGGGGGAGGTGGGGTTGATGGGTTTCCTTATCCCCAAAGTCACTCCT1520.11571166480157734No Hit
GGCAGTGGGCCTGCATCACAGGTTGCAGCAGATGTTCTCAATATTTCTATTA1520.11571166480157734No Hit
TATAAAGGGGGAAGGTTCGGGAGGCTTCTGGAAAAAGCGCCGCGCGCTGGGC1520.11571166480157734No Hit
TGCACGTAGGGGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTAT1500.11418914289629341No Hit
CTAGATGGGCTCGAAGTAGAGTCTAACTATTACTATTCCCATTTTACAGTGA1500.11418914289629341No Hit
ATCATGAGGCAAGCAACTACTTCACTGTATGGCCTGCAACCAACTTCTGCAA1500.11418914289629341No Hit
GTAGGTGGGACGGCGGGGTAATGATGAAGCTTATGTTTATCATTTAAAAGAA1500.11418914289629341No Hit
TGTTACGGGCGCATTTTCCCATGGTGTATGACTAGTTCATCTCTTTCTTGAG1490.11342788194365146No Hit
AATGGGATTGTTTTAAAGTAAACCAGATCTCCAAATACCTATAAAACAAACC1490.11342788194365146No Hit
GCCACCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGT1480.1126666209910095No Hit
CTTTCGCGGGATGAACTCTAATTGCCATTTGAAGCTACTGATTGGCAGTGGC1480.1126666209910095No Hit
TTCTACGGGAGGTTTTCTAAATGTGGTAGGAAAATGTACTCTGGAAAAATGC1470.11190536003836755No Hit
TATTCTGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT1460.1111440990857256No Hit
TCTGCAAGGGGAGAGAAAAATGGGGAGCATGAAAACAGAAAATTAGGGAGAA1460.1111440990857256No Hit
TGAACCGGGCAGAGAAATGAACACATAATCATGCAAAAACCTGTACACCAAT1450.11038283813308364No Hit
ATTGCAGGGTTATGCTTGTGGTCCCAGCATTTTGGGAGGCTGAGGTGGAAGA1450.11038283813308364No Hit
GTCGATGGGGATGTAAACACCCGAGCCGGGCTCCAAGGCCCGGGAGGTCAGA1450.11038283813308364No Hit
GTAAATGGGAAGAAAATGAGGAAGAGAATTAACCCCAAAGAAGCAGAAATTG1440.10962157718044169No Hit
TATATCGGGACAATCAGGTTCTGCTGCAGTTTGTTTATTGATGGGTATTTAT1440.10962157718044169No Hit
GATCTTGGGATGATGTATGCAGGATCCCAGCATTGATACCCAATGACAAACT1440.10962157718044169No Hit
TTTCTTGGGGCTTCCTCAGGCGGCGGCCATGGCGGGACAGGAGGATCCGGTG1430.10886031622779974No Hit
TACGCTGGGGCATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAA1430.10886031622779974No Hit
AATAATGGGGATCTGTGCGCGGGGTACTATCCCTATGAGGCATAATTATAAC1420.10809905527515778No Hit
TCCATAGGGAGCCTTATAAAAGGGAAGGTAAATGGCATAAATATGGTCGCAC1410.1073377943225158No Hit
TAGATAGGGTGTCAGGATGCCTTTGTTAGGATCTGTATTTGCCCTTAATTTT1410.1073377943225158No Hit
CATGTCGGGACTGCCATCACAGTCCTCATCGTGATGCAAGCTTATATGATTC1400.10657653336987385No Hit
TCATTAGGGGGTTTAGCAAACTTCTAAATAGCCCATTTTAAGGGAGAACTTA1400.10657653336987385No Hit
GCTCAAGGGTGGATGAAGAGAAGAAAATCCGGCTTCGGTTGCAGATGGAAGT1400.10657653336987385No Hit
CGAGGAGAGGATTCAGTGATTGGTATGAGATGCAAGATGCTGGAATTACTTC1390.1058152724172319No Hit
ATTATGGGGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGC1390.1058152724172319No Hit
AAACTTGGGGTAGTTATCTGAAAATAAAAGGAGAGTGCTGAAGTTGAATATA1380.10505401146458995No Hit
TAAGTAGGGGAACTTTTGGAAATTAATTTATACATAATGAAAGAACAAACTC1380.10505401146458995No Hit
CTTCTTGGGACCTCTAAATTTCAAAATTATGAAGAATTACAGAATTACTCAT1380.10505401146458995No Hit
GACACCGGGAACCAGAAAATTAATTAATCTCTAAAATTAAACTTTACACTGA1380.10505401146458995No Hit
GCTCATGGGCGATCTAAGGCGGGGGGTCAATATTCCTAGGAATGGGAACATT1380.10505401146458995No Hit
GTACCTGGGTACCTACACTTATAACAATAATAGCTATCATTTATTGGGTGTT1380.10505401146458995No Hit
GCATCCGGGGTCCTGTGGGTGCTGCTGGGCACCCCTGCAGGCTATGTTGCTG1360.10353148955930602No Hit
TTGCGTGGGAGACTGTAAATATGTAAGTCTAACCTGAAGTTTGTTTGAAAGT1360.10353148955930602No Hit
ATTTAAGGGGACCGCGAAGGGAGGAGTGGCAACATGGCGTCTTCGGGAGCTG1360.10353148955930602No Hit
GAACACGGATGTTTAAAAACAAAAACACATATTCAGAGCATTGGACTTTTTT1360.10353148955930602No Hit
GTGCACGGTGGTGGTTTGCACCAGGACAGTTGCTGTAGAGGAGGTGAACAAA1360.10353148955930602No Hit
TCGCGAGGGAGTTGTTGGATTTATAACTTTATTGAGGAATTAATTTGATTCT1360.10353148955930602No Hit
AACCTAGGGGACCTGTCCTGCAGCACTGGATGCTTTGTGAGTTGGGGATTGT1360.10353148955930602No Hit
ATGGCGGGCTCTTTAAAAGTGTTCTTTAACACTGACTACTAAACTTTAAGTT1350.10277022860666407No Hit
GTTAATGGGATTGTGACTTTTTTTTTCTTGAAACTCTTTTTTCTTTTATCTT1350.10277022860666407No Hit
AAGACAGGGACTTATAATTGAGAAAGAATGTGGTGGGGGGAAGGGTGGTAGT1350.10277022860666407No Hit
TGCTGAGGGGGCCATCCACAGCGAAGCCTTCAAAAATCCCCACGCCCCAGAG1350.10277022860666407No Hit
AGGACTGGGTGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCC1340.10200896765402212No Hit
GTACTTGGGGAAGAGCCTGGTCTCAGTTACCAAGGAGGGTCTGGAGCTGCCT1340.10200896765402212No Hit
CAACACGGGGCGAACGGGCGGGCGAGCAAGCGAGCGGCGTCTCCACCAGCAT1340.10200896765402212No Hit
TTTACTGGGAGTCCCACAGGTCCCTAAAGCTCTGTCCATCTTGTTTTCCCAT1340.10200896765402212No Hit
TATCAGGGATGGCACAAGGAACTGAAAGTAACTATTGGCTGGCTTAGGTGAG1340.10200896765402212No Hit
AGACTGGGGGGTGTATTATGAAATCATAAAACATTAATGAGACATGATATCT1330.10124770670138017No Hit
TCCAGTGGGTCTGACATCTCCTGCATCGCAGAGGCCACTGCCAGCCCCCCTT1330.10124770670138017No Hit
TAAGGAAGGGGTCTTTCAGTTCTCCAAAAGCCCAATTTAATTGTATAGTTTT1330.10124770670138017No Hit
TTACGACGGGGGCCAAGAACAAAAGAAAGATGCTTAATCTCACCACTTTTTT1330.10124770670138017No Hit
GGCATTGGACTTTTTTTAACTTGTTTTCATCTGTTTATCATGACTTTTTTAT1330.10124770670138017No Hit
GATAGCGGGGTTCGAGGTTGCAGTGAGCCGAGGTCGCACCACTCCACTCCAG1320.1004864457487382No Hit
AGCTTGGGGGTTAGCTGTGTTTCCAACTGTAATAATTAGTGAGCCTGGAGGG1320.1004864457487382No Hit
GTAGAGGGGAGTCAAGATGGCGGGAGCAGCTACCCAGGCTTCCCTGGAGTCG1320.1004864457487382No Hit
ACAGTAGGGTCTCCTTCCTCATATCCAGTCAGTCATCAAGTCCTGTCTGTTT1320.1004864457487382No Hit
ATTGAAGGGCGATCTATGCATGGGGCATCAGGGATAAGGAAAGGTTAAAAAA1320.1004864457487382No Hit
CGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCA1320.1004864457487382No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)