FastQCFastQC Report
Sat 31 Dec 2022
SRR5847298.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847298.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234554
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC7200.3069655601695132No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT5950.25367292819563936RNA PCR Primer, Index 23 (95% over 21bp)
GTTAGCGGGTACTGCATACGCCAAAGAAAGATCACCTACTGCATACGCCAGA2500.10658526394774763No Hit
TTCAGGGGATAATTACAATAGTTGCTTTAAAATCTGTTTCATTTAATGTCTT2430.10360087655721072No Hit
AGTACTGGGAGCTGACATTTTACTTTGGGGACTAAAAATCTCCTTTTTTTTT2400.10232185338983774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)