FastQCFastQC Report
Sat 31 Dec 2022
SRR5847292.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847292.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195652
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC5530.28264469568417394No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT5260.26884468341749634RNA PCR Primer, Index 23 (95% over 21bp)
GCACTTGGGGATAGAGCTGTAGAAATCCAGAATAAACCAGAGAAAGACACAG2390.12215566413836812No Hit
GTTCTGGATACACAGTAGATGCCTAATAAATGTTTATCTCAAAAGTGATGTT2350.12011121787663812No Hit
CGGATAGGGATGAAGGGAAAGATAGTCTTTTCCAAAACAAATGCTGAAAGAA2250.11500010222231308No Hit
GCGCATGGGGGTGCTATATCAGGTGAGAAGACTTTTTCAAAGTGAAAATAAA2240.11448899065688058No Hit
TGACTAGGGTTTCATGGAAAAGATGAGCTTGTTCTTTTGCATCAGCAAGTTG2230.11397787909144808No Hit
ACTATTGGGAAGTTTTTATGATCATCTTAATATAAATAACTGGCAAAGATTT2220.11346676752601557No Hit
AAAGTAGGGGAAAATACTAAGAAAATCAGATGAATTGAATGTTGAGAAAGTA2190.11193343282971807No Hit
AATTTCGGGAGAGGAATAATTCACAGTGGAGATGGGGTAGATAGTTTTGTAG2180.11142232126428557No Hit
CATTATGGGCCACTTAACCAGCTATGTGATTTTAAATAAGTTTCTTAATTTA2120.10835565187169054No Hit
TGATACGGGCAACAGGAAAAATGTTTCTAGTTCATCAATTCACTTAGGAAAT2120.10835565187169054No Hit
TAGCCGGGACAAACAGACATGGCAGAGCAGTTTGCTTTGGGTTGGCAGAAAG2090.10682231717539303No Hit
TGAAAAGGGTCATATTAAGTGAAATAAACCAGGAACAGAAAGTTCTCATTCA2050.10477787091366303No Hit
AAGCCTGGGATGGTCTTTCCATGTTTTCTATTTGTTAGAGGCTATCCACTAC2050.10477787091366303No Hit
TTGTCGGGGATTGTAGTTTTGATGTGCATTTCTCTGATGATCAGGAGATCCA1990.10171120152106802No Hit
AATACCGGGGGAAAGAAGGTAATTTCCCAAGTGGAAAATTGGTTGTCTTTGA1990.10171120152106802No Hit
GACTCTGGGATTGGTTATTATTTTTTAAGCACTTATTATGGGTGCTTATTAT1970.10068897839020301No Hit
TAGCAAGGGGAGAATCAAGTAGAAAACATCCTGCCATTCACTGTAAAAAATG1970.10068897839020301No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)