FastQCFastQC Report
Sat 31 Dec 2022
SRR5847291.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847291.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197332
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT5390.27314373745768555RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC5310.2690896560111893No Hit
GTTCTGGATACACAGTAGATGCCTAATAAATGTTTATCTCAAAAGTGATGTT2540.12871708592625625No Hit
ACTATTGGGAAGTTTTTATGATCATCTTAATATAAATAACTGGCAAAGATTT2320.11756836194839154No Hit
CGGATAGGGATGAAGGGAAAGATAGTCTTTTCCAAAACAAATGCTGAAAGAA2300.11655484158676747No Hit
TGAAAAGGGTCATATTAAGTGAAATAAACCAGGAACAGAAAGTTCTCATTCA2190.11098047959783512No Hit
TTGTCGGGGATTGTAGTTTTGATGTGCATTTCTCTGATGATCAGGAGATCCA2170.10996695923621105No Hit
ACTCGGGGGAATAGGTTTGATTTATATTGCCTTCATCATTCTGGTTTCAGAC2140.10844667869377496No Hit
GGCTCTGGGATAGCCAATTTAGGCTAATGCTTGGTTTTGGAGCTTTTATACA2120.10743315833215089No Hit
TGACCTGGGCATTTTACAGATGATCGTAGATTCCTGCTAAAATCTCACATGA2100.10641963797052682No Hit
CGTGAAGGGAACACTAAAATTCAAACAAATCAAATAGAAAATATACGTTGCA2080.10540611760890277No Hit
TAAAGGGTAAGAAGTAACTTAAGATGGATCAAATACCTAAATGTAAGCTAAA2080.10540611760890277No Hit
TACAGGAGGGGTAATCCAAGTAAGATGTTCACAAAGATTTGTTTTTAATGTC2080.10540611760890277No Hit
TGACGCAGGGACTTCTTGAGTTTCTTGTTAATGATGGAAGATCCACCTTTAT2070.10489935742809073No Hit
CACTTTGGGGGCAACAAAAGCCAAAATTGACAAATGGGATCTAATTAAACTA2050.10388583706646666No Hit
TAGCCGGGACAAACAGACATGGCAGAGCAGTTTGCTTTGGGTTGGCAGAAAG2050.10388583706646666No Hit
TGCTATGGGAACATCCATTGTCCCAATTGTAAAATAAAGAGAAAATCTATAC2050.10388583706646666No Hit
TGACTAGGGTTTCATGGAAAAGATGAGCTTGTTCTTTTGCATCAGCAAGTTG2050.10388583706646666No Hit
GCACTTGGGGATAGAGCTGTAGAAATCCAGAATAAACCAGAGAAAGACACAG2030.10287231670484259No Hit
TGAACTGGGAACTCTGTAAGATAGAAGAAGCAAGTAGAACCATTCCCAAGTT2020.10236555652403058No Hit
CATTATGGGCCACTTAACCAGCTATGTGATTTTAAATAAGTTTCTTAATTTA2020.10236555652403058No Hit
GCGCATGGGGGTGCTATATCAGGTGAGAAGACTTTTTCAAAGTGAAAATAAA2020.10236555652403058No Hit
TCATGAGGGGATCTTAGCTCGGGCCACCTTTCTGGGTTTTGCTTTCCAGGAC2020.10236555652403058No Hit
AATACCGGGGGAAAGAAGGTAATTTCCCAAGTGGAAAATTGGTTGTCTTTGA2000.10135203616240651No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)