FastQCFastQC Report
Sat 31 Dec 2022
SRR5847290.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847290.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196147
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC5400.27530372628691746No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT5010.2554206793884179RNA PCR Primer, Index 23 (95% over 21bp)
GTTCTGGATACACAGTAGATGCCTAATAAATGTTTATCTCAAAAGTGATGTT2270.11572952938357457No Hit
CGTGAAGGGAACACTAAAATTCAAACAAATCAAATAGAAAATATACGTTGCA2170.11063131223011313No Hit
CGGATAGGGATGAAGGGAAAGATAGTCTTTTCCAAAACAAATGCTGAAAGAA2170.11063131223011313No Hit
TACAGGAGGGGTAATCCAAGTAAGATGTTCACAAAGATTTGTTTTTAATGTC2170.11063131223011313No Hit
TTGTCGGGGATTGTAGTTTTGATGTGCATTTCTCTGATGATCAGGAGATCCA2140.10910184708407471No Hit
GGAGTAGGGACCTGGTCTACATGCAATGAAAATTAGAAATTCAACATACACA2110.10757238193803625No Hit
TAAAGGGTAAGAAGTAACTTAAGATGGATCAAATACCTAAATGTAAGCTAAA2100.10706256022269013No Hit
AATTTCGGGAGAGGAATAATTCACAGTGGAGATGGGGTAGATAGTTTTGTAG2090.10655273850734398No Hit
AAGCCTGGGATGGTCTTTCCATGTTTTCTATTTGTTAGAGGCTATCCACTAC2090.10655273850734398No Hit
CAAAAGCGGGAAATGGATCTGCTAAATATTCAACAGGTATATGTGGACTTTT2080.10604291679199784No Hit
ACTATTGGGAAGTTTTTATGATCATCTTAATATAAATAACTGGCAAAGATTT2080.10604291679199784No Hit
GTATGCGGGCAAGCTTTATAGGTGCAAAGAAGAAAAGAAATTTGAAGTAAAA2060.10502327336130557No Hit
CGTATAGGGAATCTTTTCATGACAAAAGAACTACTCAGTGCTGAAGCAGTGT2030.10349380821526712No Hit
TGACGCAGGGACTTCTTGAGTTTCTTGTTAATGATGGAAGATCCACCTTTAT2020.10298398649992097No Hit
GTCAGGGGGTCTGGATTGGAGTTCTTTGTTAAGTTATATATGCGTTGCAGAT2000.10196434306922869No Hit
TGGCTGGGAGGAAAACTTCCTACTTGGGCTTTTCAGGCTTATGTGACATTAT1990.10145452135388254No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)