FastQCFastQC Report
Sat 31 Dec 2022
SRR5847286.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847286.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151959
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT4680.3079778098039603RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC3820.2513835968912667No Hit
CAAAGAGGGGGTGATGAATGCTGGTCAAGGGGCCAAGGTGGAAAAGTGAGGG2040.1342467376068545No Hit
GCATTGGGGATGAAAAGATTCTCTGGAATCCTTGAAAGACTTAATAGAGATA2030.1335886653636836No Hit
GTAAATGGGACAGAAACAACAGCCAAGCCAAGAGCCAAATCAGGAATGCAAA1980.13029830414782934No Hit
AGTTAAGGGAGGTGATTGATTTGAAAGGAAAATGTAAACATGTATAAAATGG1960.12898215966148766No Hit
ATTACAGGGATTCAGGCCCAGTTTATAAACTGGGATCAAGATGTGCATTATA1850.12174336498660823No Hit
GCGATGGGCCGATCTACAAGTGGGAGGGTGGAAGCCTGGCGCACGTCCGGAG1810.1191110760139248No Hit
CCTGCCTCAGCCCACCAAGTAGCTGTGCCACCACACCCGGCTAATTTTTTTG1810.1191110760139248No Hit
AATCAAGGGGATGCCACACATTCTCTCTCAATTTCAGCTTCGGAACGCTATG1800.11845300377075396No Hit
GCCAGCGGGCAATGAAGCATCAATCAACCAAACAACCCTGGAGCTGAGCACA1790.11779493152758311No Hit
TGACGCGGGCACTGGGCTCAGATACCTGCTATACAACTTCCCGATGACCTGC1780.11713685928441224No Hit
GGGTACGGGGAGCCAGCTTTTGAGATTCAAAACTGATCAATGGCTTCTTTGT1770.11647878704124139No Hit
TTTGCTGGGGAAATGAACATGTGTATATTTTCTACACCTATTATTTAATTTC1750.11516264255489969No Hit
ACAATGGGGAGTGCCACTGGAAAGCGGCAGACCTGTGACATCGAAGGCCTGG1730.11384649806855797No Hit
CGACTTGGGCGATCTGTGGTGGGGATTTATTCTGTTCAAGGTTGAAGTGGAC1720.1131884258253871No Hit
TCCTGGGACCTCCTCAGTGGCTACTAAACCATTTTCCCCAGACCTCTTTCCT1710.11253035358221625No Hit
GACAATGGGATCTACATATGGGGGTATGGAAGGCTTTGTTACAAGAAATGCC1710.11253035358221625No Hit
AAACTTGGGTTGGAGAAAGGCTGATGTTGCATACAAACACTGTGACTGTCAC1670.10989806460953283No Hit
TTCTTTGGGCGATCTGTCATGGGACGGATGGTGCCCAGGCCCCTGGAAGCAG1670.10989806460953283No Hit
TCAGCGTGGGATTGAAGTAGAAAAGCCGAAGATACACGTACAGTCATCATCT1660.10923999236636198No Hit
ATACATGGGGGCTCACAACAAGCCCCAGTGAGATGAACCCGGTACCTCATTT1640.10792384788002028No Hit
CAACACGGGTCTTCTTAACGGGTGGATCTTGAAGACTTAGTTTGGAAAAAGG1630.10726577563684941No Hit
TGCGCTGGGATACAAAAAGCAAAACAAAACCCTCAGCACTGTTACAAGAGGC1630.10726577563684941No Hit
AGCTGGGATCATGGAGATTGATGATATAAGTTTATTTTTATGATGACTTTAT1610.1059496311505077No Hit
GTCGAAGGGACCGATTTTCTTTTTAATACTACCTGGTGTTTTTACTTAGATA1610.1059496311505077No Hit
TTTGCCGGGCACATACACATATACACACACAACCCCTTAGAAGTTTATTACA1610.1059496311505077No Hit
AATTTTGGGAGGCGTGGGATCTGGTTCCTGATGCTTAATCCTTTGCTCTTTC1600.10529155890733684No Hit
CAAATAGGGCCGGGACACTACCAAGATCATGATAGTGAATAAAATTTTTCCA1590.104633486664166No Hit
TACCATGGTACTGGAAACATCAGATTGTCAATTTCTTCCTTATTTGCAAATA1590.104633486664166No Hit
CGATGCGGGATGTTCATCCTTGTAAATTTACGTTTAAAATATGTTAAAACTT1590.104633486664166No Hit
TAGTTAGGGGAAGGGAAGAGGAAACAGAGACGCAGGGAGGAGATGGCCATGT1580.10397541442099514No Hit
ACTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1580.10397541442099514No Hit
CTAGCCGGGCGATCTATCGTTGGGGTTTGTTAAAGAGTCATTTTTAATGAAA1570.10331734217782429No Hit
GATAGAGGGACAGGATACCAGTGTCCCCATATGAAAATTATATATTTTAAAG1570.10331734217782429No Hit
TGCGTGGGGATTCACCTTAGGTAAACCTAGTCAAGTCCAATGACTAAATTTC1550.10200119769148257No Hit
TCTAAAGGGCTGCCTTTGTGCTCTGTTCCTGTCCAGCAGGGGTCTCCCAACA1550.10200119769148257No Hit
GATGCACGGGGATCTAAATAAATGGAAAAACATCTGTGTTCATGGATCAGAA1550.10200119769148257No Hit
AACCTAGGGTACAGACAAATTCAGGGAACATATGTGGGACTGGATACTAAGA1550.10200119769148257No Hit
AATCAAGGGTCTTCTCTATTGTAGAGAATGATTTTTGTTTGCTTCATTATGT1540.1013431254483117No Hit
ACGCGTGGGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTT1540.1013431254483117No Hit
ATAAACGGGAAGTACCTTATAATAACAAAATATTCCTAAATTCATTTATTCC1540.1013431254483117No Hit
TTGTTGGGGAAGTTTCTCTTCGGTGTAACAATAGGATATGTGCATGTTTTTG1540.1013431254483117No Hit
GGACAAGGGGGGAAAGAGAAGTAAAGAGTAAAGCCAGAGGCCATTACCTGAA1540.1013431254483117No Hit
CACAACGGGTGGTTTCTTATGTTAAATGTATTGTGCTTCCCTTCTGTCTCTA1540.1013431254483117No Hit
CAAAGAGGGAACGTTTTCTTTTTCAAATACGTAGCCAGTTGTCACATCATTT1530.10068505320514087No Hit
CTGAACGGGAGCCACAGTGCAGCTCCACATTAACTCTACAGACCAAACCATT1530.10068505320514087No Hit
ATGCAAGGGGTAGTCAACGATTAAGTGAGAAGGAGGAATTACTGGCTGACTT1530.10068505320514087No Hit
GAGCATGGGGTCTGATCTCATATGCAGAAATGAACCACAGAGCATCAAGGAA1520.10002698096197No Hit
ATCTTGGGGTCTGTGTGCGGGGAAAATATCATTCTATAACAAGAATCAATTT1520.10002698096197No Hit
GCCATTGGGTCCCATTTTCCTCAACAAGTCAAAACATGCTTTCATTGTTTTA1520.10002698096197No Hit
CACATGGGGAGAACGGCTTCCTCCCAATTCACCTGTTGGGCCTCCGAGTGGA1520.10002698096197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)