FastQCFastQC Report
Sat 31 Dec 2022
SRR5847285.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847285.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153020
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT4590.29996078943928894RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC3860.25225460724088355No Hit
CAAAGAGGGGGTGATGAATGCTGGTCAAGGGGCCAAGGTGGAAAAGTGAGGG2110.13789047183374722No Hit
GTAAATGGGACAGAAACAACAGCCAAGCCAAGAGCCAAATCAGGAATGCAAA2000.13070186903672723No Hit
GCAAATGGGAAAGAACAACATGCTTAAAGCAATTGCATTTCTGTATTCCTAC1980.12939485034635995No Hit
GCGATGGGCCGATCTACAAGTGGGAGGGTGGAAGCCTGGCGCACGTCCGGAG1950.12743432231080903No Hit
ATTGTGGGCGGCAGCCGAGTGACAGTGCGTGGTCCCCACCATCAGATCAGCC1860.12155273820415632No Hit
ATACATGGGGGCTCACAACAAGCCCCAGTGAGATGAACCCGGTACCTCATTT1820.11893870082342177No Hit
CGACTTGGGCGATCTGTGGTGGGGATTTATTCTGTTCAAGGTTGAAGTGGAC1810.11828519147823813No Hit
GGACAAGGGGGGAAAGAGAAGTAAAGAGTAAAGCCAGAGGCCATTACCTGAA1800.1176316821330545No Hit
TTGTTGGGGAAGTTTCTCTTCGGTGTAACAATAGGATATGTGCATGTTTTTG1790.11697817278787087No Hit
CTAGCCGGGCGATCTATCGTTGGGGTTTGTTAAAGAGTCATTTTTAATGAAA1780.11632466344268723No Hit
TTCTTTGGGCGATCTGTCATGGGACGGATGGTGCCCAGGCCCCTGGAAGCAG1780.11632466344268723No Hit
AGTTAAGGGAGGTGATTGATTTGAAAGGAAAATGTAAACATGTATAAAATGG1760.11501764475231996No Hit
GGTTCAGGGTGCTGAATATTAGAGTGGTTGTTTCAGAGAGTTTGCAGCAATT1750.11436413540713633No Hit
TCCTGGGACCTCCTCAGTGGCTACTAAACCATTTTCCCCAGACCTCTTTCCT1740.11371062606195267No Hit
TAGTACGGGAGAGCTACCCCACCAAGAAGGCTCCAGGAACTGGGACTGGGGA1730.11305711671676905No Hit
CATTCCGGGGCCTCATTAGCTTACTATTGCCATCCCCCTACATCTTGGACTC1730.11305711671676905No Hit
TGCGTGGGGATTCACCTTAGGTAAACCTAGTCAAGTCCAATGACTAAATTTC1720.1124036073715854No Hit
AAACGAGGGAATATGTCAAAGTCTGCTTTGTAGTAAAGTAACATAAAAAATA1720.1124036073715854No Hit
ACAATGGGGAGTGCCACTGGAAAGCGGCAGACCTGTGACATCGAAGGCCTGG1710.11175009802640178No Hit
ATTACAGGGATTCAGGCCCAGTTTATAAACTGGGATCAAGATGTGCATTATA1700.11109658868121815No Hit
TGTAATGGGGCCATTAATATTGGTTCAATGAAAAATTTTAGAAGCTATGTTA1700.11109658868121815No Hit
GAGGCAGGAGAATGGTGTGAACCTGGGAGGCAGAGCTTACAGTGAGCGGAGA1690.1104430793360345No Hit
TGCGCTGGGATACAAAAAGCAAAACAAAACCCTCAGCACTGTTACAAGAGGC1690.1104430793360345No Hit
ATCGACGGGACACTCAAAGCAGGAAAATGTATTTCTGACCCAACAAAAGTCT1680.10978956999085088No Hit
TGACGCGGGCACTGGGCTCAGATACCTGCTATACAACTTCCCGATGACCTGC1680.10978956999085088No Hit
GTTCTTGGGACAGAAGAAAAACTGTAATCCTGGGGTTGGTATGTAGGAGGTC1680.10978956999085088No Hit
TCCGAGGGGGTTTGCCATTATTTTTCAGGAAGCTGGCCATGTTAATTTTCTG1680.10978956999085088No Hit
TTTGGGGGGGCATATATAATTGCATATTACTAATTATTAGTCATGTGAGCTA1680.10978956999085088No Hit
GTGCTGGGATTACAGGCATGAGTCACCGCACAGGGCCAAACAAACAAACAAA1670.10913606064566723No Hit
CACATCGGGTAAATTATAAAAATAAATGTAACATACCAAAACATTCATATTA1670.10913606064566723No Hit
AAGTATGGGAAGAGTCAATTGAATGAAGGCCATCACTTAAAACCAAGACTTA1670.10913606064566723No Hit
TCTAAAGGGCTGCCTTTGTGCTCTGTTCCTGTCCAGCAGGGGTCTCCCAACA1670.10913606064566723No Hit
TCAGCGTGGGATTGAAGTAGAAAAGCCGAAGATACACGTACAGTCATCATCT1660.1084825513004836No Hit
TGTATTAGGGGAAGATGGGCTCCCTGGAGCGAAAGAAATTACGGTATTCAGA1660.1084825513004836No Hit
CGATAAGGGAGACTTCGGTTGAGGAAACGGGTATTTCATGTCTCAGGGAGTA1660.1084825513004836No Hit
AGGCCAGGGGGCTTCTAGAACCAGAGGCATTCAAACAGCCCTAAATTATCAA1640.10717553261011632No Hit
AGCTAAGGGATAGATCCAACTCTGTCCTAAATAACTAGGATAAACTAGCTTT1640.10717553261011632No Hit
AAATGCGGGCCCGGCTCTCCGTGCCCGCCGCGCTGGCCCTGGGCTCAGCCGC1630.10652202326493268No Hit
GTCATTGGGAATAGTTTTCTGAAATAGAAAAATTTACTGTTATTTTTATTAA1620.10586851391974905No Hit
GCCATTGGGTCCCATTTTCCTCAACAAGTCAAAACATGCTTTCATTGTTTTA1620.10586851391974905No Hit
CTGAACGGGAGCCACAGTGCAGCTCCACATTAACTCTACAGACCAAACCATT1620.10586851391974905No Hit
CACAACGGGTGGTTTCTTATGTTAAATGTATTGTGCTTCCCTTCTGTCTCTA1620.10586851391974905No Hit
GACAATGGGATCTACATATGGGGGTATGGAAGGCTTTGTTACAAGAAATGCC1620.10586851391974905No Hit
GAAAAGGGACTCAGATGCTGGAGAGCATGATCAAGAAGCCCCGCCCCACTCG1610.10521500457456542No Hit
AATTTTGGGAGGCGTGGGATCTGGTTCCTGATGCTTAATCCTTTGCTCTTTC1610.10521500457456542No Hit
AACCTAGGGTACAGACAAATTCAGGGAACATATGTGGGACTGGATACTAAGA1610.10521500457456542No Hit
TGCTTTGGGAAGTGAGAATGAAAAAACAAAATTCCAAGACACTTTTTAAAAG1610.10521500457456542No Hit
AGCTGGGATCATGGAGATTGATGATATAAGTTTATTTTTATGATGACTTTAT1600.10456149522938178No Hit
GCGACCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1600.10456149522938178No Hit
ATTCCGGGGGATGGTCATAGATCTGGAAAAACAGATTGAAAAACGAGACAAA1600.10456149522938178No Hit
CTCGGTGGGAGATAATGTGTCGATAGCTTCATCTTGCCCGTAGAAAACAGTT1600.10456149522938178No Hit
CTGTATACGGGAGGCTCAACAATAAAAGTATGAGCAACAACAAAAATCTATA1600.10456149522938178No Hit
GCATTGGGGATGAAAAGATTCTCTGGAATCCTTGAAAGACTTAATAGAGATA1590.10390798588419815No Hit
TTTGCTGGGGAAATGAACATGTGTATATTTTCTACACCTATTATTTAATTTC1590.10390798588419815No Hit
GTAAGCGGGGGAGGCCTGGTACTCCTCCAGGTTATCTGGCCAGTGTAGACTC1590.10390798588419815No Hit
GTTTGCGGGTTATTAAACCCCGATCATAACCTCCAGCAGGCATTTCATTTAA1590.10390798588419815No Hit
AAATGTGGGTCAGCTCACTGCAACCTCCACCTCCCGGGTTCGAGTGATTCTC1590.10390798588419815No Hit
ATATAAGGGGGACGAACATAGAAATGAAAAAAGACAAAAGAAACTGTTTTAA1580.10325447653901451No Hit
CACGCTGGGAGAGATTTACCCGAGCGTGTTGCGGCAGCGGCTGGGCTTGCAA1580.10325447653901451No Hit
CAACACGGGTCTTCTTAACGGGTGGATCTTGAAGACTTAGTTTGGAAAAAGG1580.10325447653901451No Hit
TAATGAGGGAATTTGAAGGAGAAAAAAAGTTATTTTATGCTGTCCATCTCAC1570.10260096719383087No Hit
TTTGCTGGGGACACAAATAACTTTTTCATGTACTTCAGTCTTGGAAGTTATC1560.10194745784864723No Hit
CAAAGTGGGCGAAAGAGATTTTCCAAGGGCTGGGTAAATCTAAGGAAGATGT1560.10194745784864723No Hit
TTTGCCGGGCACATACACATATACACACACAACCCCTTAGAAGTTTATTACA1560.10194745784864723No Hit
CCTGCCTCAGCCCACCAAGTAGCTGTGCCACCACACCCGGCTAATTTTTTTG1560.10194745784864723No Hit
CCGCGTGGGAAGGATTTTCAGTGCCATTTAGCAAAACGTCTTTAGTCTATGC1550.1012939485034636No Hit
GTCAAAGGGACTGTGGTATAGATTCCATAATATGCTTGAATATTATGATATA1550.1012939485034636No Hit
TGCTACGGGAATGTAAATCAGTTACTTAGTCATTGACTGTGACAGATGACAG1540.10064043915827996No Hit
TTAATGGGTGGCGGACCATCCATGTCAATTTATGAGAAAAAAATTCATTCTG1540.10064043915827996No Hit
TGACATGGGTTTCTGTGCCTAGAAGGTAAAGATTTTAACAGAAGAATACGAA1540.10064043915827996No Hit
TGCATGGGACTTGACAAAGGTGGATAAATTAGATGCTTCAGAATCACTGAGA1540.10064043915827996No Hit
TTATTAGGGCGATCTAATTGTGGGAACTGTTAGGAGGTAGGGCCTTTGGGAG1540.10064043915827996No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)