FastQCFastQC Report
Sat 31 Dec 2022
SRR5847284.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847284.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150086
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT4590.305824660527964RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC3540.23586477086470423No Hit
CAAAGAGGGGGTGATGAATGCTGGTCAAGGGGCCAAGGTGGAAAAGTGAGGG1970.13125807870154443No Hit
GTAAATGGGACAGAAACAACAGCCAAGCCAAGAGCCAAATCAGGAATGCAAA1960.13059179403808482No Hit
ATTGTGGGCGGCAGCCGAGTGACAGTGCGTGGTCCCCACCATCAGATCAGCC1940.1292592247111656No Hit
TTAATGGGTGGCGGACCATCCATGTCAATTTATGAGAAAAAAATTCATTCTG1930.128592940047706No Hit
ATTCCGGGGGATGGTCATAGATCTGGAAAAACAGATTGAAAAACGAGACAAA1800.11993123942273098No Hit
TGTAATGGGGCCATTAATATTGGTTCAATGAAAAATTTTAGAAGCTATGTTA1780.11859867009581172No Hit
CACAACGGGTGGTTTCTTATGTTAAATGTATTGTGCTTCCCTTCTGTCTCTA1780.11859867009581172No Hit
GATGCACGGGGATCTAAATAAATGGAAAAACATCTGTGTTCATGGATCAGAA1740.11593353144197327No Hit
AGTTCTGGGGCATGAAAGAACGTGAGCACGGAGAAAAGGAGAGGCAGGTGTC1720.11460096211505404No Hit
GCCATTGGGTCCCATTTTCCTCAACAAGTCAAAACATGCTTTCATTGTTTTA1710.11393467745159443No Hit
GCAAATGGGAAAGAACAACATGCTTAAAGCAATTGCATTTCTGTATTCCTAC1690.11260210812467518No Hit
GCATTGGGGATGAAAAGATTCTCTGGAATCCTTGAAAGACTTAATAGAGATA1680.11193582346121557No Hit
GTATGCTGGGGCCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAA1680.11193582346121557No Hit
TCCGAGGGGGTTTGCCATTATTTTTCAGGAAGCTGGCCATGTTAATTTTCTG1670.11126953879775595No Hit
CCTGCCTCAGCCCACCAAGTAGCTGTGCCACCACACCCGGCTAATTTTTTTG1670.11126953879775595No Hit
ATGCAAGGGGTAGTCAACGATTAAGTGAGAAGGAGGAATTACTGGCTGACTT1670.11126953879775595No Hit
GACAATGGGATCTACATATGGGGGTATGGAAGGCTTTGTTACAAGAAATGCC1660.11060325413429634No Hit
TTTGCTGGGGAAATGAACATGTGTATATTTTCTACACCTATTATTTAATTTC1650.10993696947083673No Hit
GGGTACGGGGAGCCAGCTTTTGAGATTCAAAACTGATCAATGGCTTCTTTGT1640.1092706848073771No Hit
TGCGTGGGGATTCACCTTAGGTAAACCTAGTCAAGTCCAATGACTAAATTTC1640.1092706848073771No Hit
TCCTGGGACCTCCTCAGTGGCTACTAAACCATTTTCCCCAGACCTCTTTCCT1640.1092706848073771No Hit
TGCGCTGGGATACAAAAAGCAAAACAAAACCCTCAGCACTGTTACAAGAGGC1630.10860440014391749No Hit
TCAGCGGGGATGTCAAGTATGATTACTGAAAACAAAAAGTTAGTGCCCATTA1620.10793811548045788No Hit
GCGCCTGGCTATATTTTACTATTTGGAAATCACAATGCATCTTAAAATTGAT1620.10793811548045788No Hit
TTCTTTGGGCGATCTGTCATGGGACGGATGGTGCCCAGGCCCCTGGAAGCAG1620.10793811548045788No Hit
TAGTACGGGAGAGCTACCCCACCAAGAAGGCTCCAGGAACTGGGACTGGGGA1610.10727183081699825No Hit
CGATAAGGGAGACTTCGGTTGAGGAAACGGGTATTTCATGTCTCAGGGAGTA1610.10727183081699825No Hit
TCTACGGGGCACCGCGCCCGGTCCCAGAAACGATTCTTGTTCATTTCTTTTC1600.10660554615353864No Hit
CGTGAAGGGATGGAGATGCACAATGATTATTCAAACAAGCTATATATATGTA1600.10660554615353864No Hit
AGGAAGGGGGGGATCTGTGGGGCGATGCAAGTGGCCTCTTGAGAGCTCCATT1590.10593926149007901No Hit
TATTCGTGGGGGAAGTACAGCAGAGGCCTTAGAGTTGATCTCTCTAATGTAT1590.10593926149007901No Hit
GCGATGGGCCGATCTACAAGTGGGAGGGTGGAAGCCTGGCGCACGTCCGGAG1590.10593926149007901No Hit
CGCCCCGGGCCGGAGGCGGGAGCTGCTTCACACTGAGGTTCAGAACCTCATG1590.10593926149007901No Hit
CTAGCCGGGCGATCTATCGTTGGGGTTTGTTAAAGAGTCATTTTTAATGAAA1580.1052729768266194No Hit
AGCTAAGGGATAGATCCAACTCTGTCCTAAATAACTAGGATAAACTAGCTTT1580.1052729768266194No Hit
AAACGAGGGAATATGTCAAAGTCTGCTTTGTAGTAAAGTAACATAAAAAATA1580.1052729768266194No Hit
TAATGAGGGAATTTGAAGGAGAAAAAAAGTTATTTTATGCTGTCCATCTCAC1570.10460669216315979No Hit
GCGCGTGGGCATTTGAAATAGCAAATACTATTTCCACATTGTGACTATCTAT1570.10460669216315979No Hit
CGACTTGGGCGATCTGTGGTGGGGATTTATTCTGTTCAAGGTTGAAGTGGAC1570.10460669216315979No Hit
TCACGCGGGTATCTTCAGGAAAACGGCATTGACTGCCCCAAATGCAAGTTCT1560.10394040749970017No Hit
TCAGCGTGGGATTGAAGTAGAAAAGCCGAAGATACACGTACAGTCATCATCT1560.10394040749970017No Hit
TTTCCTGGGTTCATTATTGCAGCCCTAGCAGCACTCCACCTCCTATTCTTGC1550.10327412283624056No Hit
ATATAAGGGGGACGAACATAGAAATGAAAAAAGACAAAAGAAACTGTTTTAA1540.10260783817278095No Hit
CTGTATACGGGAGGCTCAACAATAAAAGTATGAGCAACAACAAAAATCTATA1540.10260783817278095No Hit
AGACATGGGAATGATGATTTGTGCAAATACTATCAAGGAAAGGACATGAATT1530.10194155350932133No Hit
CTAAACGGGAATCTCAGAGTGATCCTGCTGACTGCCAGCAAAGGTTTGTAAT1530.10194155350932133No Hit
TGTATTAGGGGAAGATGGGCTCCCTGGAGCGAAAGAAATTACGGTATTCAGA1530.10194155350932133No Hit
TTGATCTGGGAGGCTGAATGTGGATTATTTTTAATTTAATGTTCATATTATA1530.10194155350932133No Hit
TATGATGGGCTGAAATAAATTATAGTACATAAAATTTTGAATTGACAATAAA1530.10194155350932133No Hit
GCCAGCGGGCAATGAAGCATCAATCAACCAAACAACCCTGGAGCTGAGCACA1520.10127526884586172No Hit
TGACGCGGGCACTGGGCTCAGATACCTGCTATACAACTTCCCGATGACCTGC1510.10060898418240208No Hit
CTACGAGGGGACTGTGCATTTGTGATTTCCTTTAGGATGCCATTTCCATTTA1510.10060898418240208No Hit
AAATGTGGGTCAGCTCACTGCAACCTCCACCTCCCGGGTTCGAGTGATTCTC1510.10060898418240208No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)