FastQCFastQC Report
Sat 31 Dec 2022
SRR5847276.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847276.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205137
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT5960.2905375432028352RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC5930.2890751059048344No Hit
AATACCGGGGGGCAGAGAGAAGTGGTCAAAATCTGGATATATTTTGGAGGTT3340.16281801917742775No Hit
TGGGCAGGGTACCCTTTAATAGAAATTTTATCTTTTGTTATTTTGGTTAATT3160.1540433953894227No Hit
ACACTCGGGATGACTCCACATGAACCTTCACATTTGTTCGCTCATAATCTAC3010.14673120889941843No Hit
CTGCCAGGGGTGGCTTAAAATAAAGAAAATGCAGCAAAAAGAATGCTATAGA2900.14136893880674867No Hit
TTGCTTGGGCAGCTACATGATATGCTCATCTACATGCACGTTTGTATGTGAA2870.1399065015087478No Hit
GATGCTGGGATCTTTTGTAATGTCATTCATAGAAAACTGGTAAATGTGTTTC2850.1389315433100806No Hit
GCTCACGGGCACATCCACACTGATCTTACCCCATAATGAACTTGGCTCTGAA2840.13844406421074695No Hit
CACATCGGGACTGCGTATGTGTCTAATATTTGTAAAATAATTAGTTCAAAAC2780.13551918961474527No Hit
GATTTAGGGGAACTAATAAAATTTTATTTCTTAAATATAAATTTGACCTAAA2750.1340567523167444No Hit
TCATGAGGGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC2720.13259431501874355No Hit
CTAATGCGGGGAGCATGGTATGTGTTTCCATTTGTTTGTGTCATCTGTGATT2710.13210683591940994No Hit
GCTTTGGGACTGAAGACAAACTGATTCTGGGCTGTGAAGATTCTTCGCTAAT2690.13113187772074272No Hit
TTCACAGGGATATGAGGTAACTCACCATCACAAAAATGACCAAAAAACGTTT2690.13113187772074272No Hit
AAGCTAGGGTAACCCAAAGAGGGAGTCCTGGGAACCCCAATTTGAAGACAGT2690.13113187772074272No Hit
TCTTAAGGGAACCTGAACATGAAAAAAATTTTATTTGTGCTTAATCAAATTA2670.1301569195220755No Hit
TTCAAGGGGGGATATCAGACTGGGAATTTTTATTTGGGACTACATCCCAGAC2670.1301569195220755No Hit
TTATTGGGGAAGCCTTCTCTGAAGTATTGAGGGAAAGTCTGCTAAATGGCTA2630.128207003124741No Hit
CAGACGGGGATCTGAAACTGTGCTGTGAGTTACTTTGCAGTCTAAAAGCAAA2600.12674456582674018No Hit
ATAAGGGTAAACGCAAAACTTTTGTACTTTATTACGAGTAAAGTGTAATGAG2590.12625708672740657No Hit
TTTATGGGGAACAGCCTTGGTGTGACTTTGTTCCAGTAAACTTTCTTTATAG2580.12576960762807296No Hit
GACTTGGGGCAGGAAACATGATATAAACAAAATTTACATAAGCCTATAATCT2560.12479464942940571No Hit
TTCCTAGGGTCGAAGCTGCCGCTGCAGACCATCATGAGGCTGCTGCAGGTGC2560.12479464942940571No Hit
TAGTTTGGGTAACAACAGAACATCAAAGTATGAGGCAAACACTGAAAGAAGT2560.12479464942940571No Hit
AATTTAGGGCGATCTAAATGGAGGGCAGTCAAGGTGAAAATTAAGCTCAATA2540.12381969123073848No Hit
TTTACTGGGAGAACTGGACTTCCTCCTCCGGGAAGCCTCCCTTGACCTTCCC2540.12381969123073848No Hit
CACTGGTGTTTATTACAGAGTTTGACATACATGGGTTCATTTGCCATTTATT2540.12381969123073848No Hit
AAAACGGGGTATGAAAAAATGTTCAGTCTCACTAGTAGTCAGGAAAATACAA2530.12333221213140487No Hit
TTTGTGGGGATGGTGAGTTGGTTTGGGAGAGGAATCTCCCAGATCCCCGTTC2520.12284473303207126No Hit
GCTAACACCTGGCAACCGCTGATTTTTTTTACTGTCTCCATAGTTTTGCCTA2520.12284473303207126No Hit
TTACACAGGGGATTTCACTTATCACATAGTATTTCACATAATATCTATTGTT2510.12235725393273764No Hit
TTGCACGGGTAACATTTCCTGTCACTGTGTGTGGGAAAAATAGATATAACCA2490.12138229573407039No Hit
GCGTGGGGTGCTATGAACAATAGCCTCTTAGAAGATAATAACAAAAATTTCC2480.12089481663473678No Hit
ATGTATGGGGAGGGCGAGAAGATCCGAAAGAAATACCCGGACCGGGTGCCGG2480.12089481663473678No Hit
CTGTCGGGTGTTGCAACATTTTTTAAAACACTGTAAAATACACCTGAAGTTT2480.12089481663473678No Hit
GTTGCTGGGCTTGGAAATAACCCCTACATACGTACCTGAGTTTCTGATTGTT2480.12089481663473678No Hit
TTGAATGGGGGATGAGAAATACAACCATATTGATGAGTCTGAAATGAAAAAA2470.12040733753540317No Hit
TACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGG2470.12040733753540317No Hit
CTCCACAAGGGAAATGTGCTACTTTTTGATACTTACATTTTCATATGCTTTA2450.11943237933673594No Hit
TTGCTAGGGACTAGGAATTGGAATCAAACAATGCCACCCCAAGCGTAATTTT2440.11894490023740233No Hit
CTCACAAAGGGATATAAGTGGAATCATACATTTGTCCTTTCATAACTGGCTT2430.11845742113806872No Hit
ACGTAGGGGCAGGATTTTATAAATTGTACTTATATGTTAGTTATGTTTCTAC2420.11796994203873509No Hit
TATGCCGGGAATCTAGTAATGGGACCAAGATAAGTAGAATTGTGTGAGAGGT2410.11748246293940147No Hit
AAAATAGGGGTCTTAGAGGAAAAAATGTAAAGGTAAGTAATTAAGCATATGA2390.11650750474073424No Hit
TCAAAGGGAAGTTCTAGATGTTATTTATAGTCTACAGTGAATTTTGGTCTGT2380.11602002564140063No Hit
AAAGCAGGGATCTCAGGCTTGTCGTCAGTGTACACAGAGCTGCTCATGAAGC2370.11553254654206702No Hit
ACTTCTGGGCACATCCAGTTATGATATGCTTGTAAGCAGGCATTAATTCTTA2370.11553254654206702No Hit
CGAATAGGGAGGCCTTATTTCATATGTTTCCTCAACTGTACAATGAACTATT2360.11504506744273339No Hit
GATCTGGGCCTTGGATTGGTAGTATAGTGAAATGATTAGGGCAGGGCTTTGA2350.11455758834339978No Hit
CTTTACGGGCTAGAAGAGAAAATTCCTAATATCCTGTCTGCATTTCCTTTCC2350.11455758834339978No Hit
TACTGTGGGATAGCAAAACCTTGTCTCTACCAGTGCACCTGTGGTCCCGCCT2350.11455758834339978No Hit
ATCAGCGGGCTGGAGGATAATGAAAAGTCCGTGTTTTGAAGTTTTCAGATTA2350.11455758834339978No Hit
TTGCAAGGGCTTGTTTTCTGTGTAGTTTACCTTAAGTCCACTCTTGGCATTT2340.11407010924406615No Hit
AGCTTGGGGACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT2340.11407010924406615No Hit
TTGCCACGGGATCTCAGATAATATATTCTAATGATGAGAATAACTACTAAAA2340.11407010924406615No Hit
AGACATGGGCTTGCTGCACATCAGGAGATCTAGAAAGGAGACTTTTCTTCCT2330.11358263014473254No Hit
TCTCTAGGGGAGGCTCAGGCTGCCATGGCTCCAGGCTCCCTCCCCTGCCTAG2330.11358263014473254No Hit
CCGGCTGGGAGTTCCTGACTTTTAAAATGGATGAAATTTTCTCTTTGTCTGA2330.11358263014473254No Hit
TATTATGGGCAAAGCAGGTGGCATTTTACTGAACAATTAAATGTTTGAAGTA2320.11309515104539893No Hit
ATGTTGGGGCAGAGAGGAATGAGAAGAAGCAGCAGATGGGCAAAGAGTACCG2320.11309515104539893No Hit
ATCCCTGGGGCTGTGAAAGATGCCGCTTCCTTGTCACTTCAGAGAATGATGC2320.11309515104539893No Hit
TCAACCGGGCTCTCAGCCCTCCTAATGACCTCCGGCCTAGCCATGTGATTTC2310.11260767194606532No Hit
CAAAATGGGCTTGACACGGGGTGGAGTGTGTGTCTTTCCTCCCTGAAGCTGT2300.11212019284673169No Hit
AAGGCTGGGACCTAACCCTTAGAAAATGAATGTTAGAAGGTGCCTGCCGAGG2300.11212019284673169No Hit
TGCTCTGGGCGAAATGCAGCTTTTTCTGAACTTCAAAGATAGAATCCCATTT2300.11212019284673169No Hit
GATAGAGGGATTGTTGAAGACGTGACTTCATAGCAATATATAGAGAATAAAT2290.11163271374739808No Hit
ATTTACGGGAGTATAACTCCTTTTAAAATGCTAATGATTCTGGAAAATGGTC2290.11163271374739808No Hit
GGTCAGGGAAATGTTTCTTTCCATTTTGCTTTGTTTTTCTATATCGATCTAG2290.11163271374739808No Hit
GATAGGGGGCAGAATAAATACAAAGATGCAGCTAACCTACTGAATGATGCCT2290.11163271374739808No Hit
TGTGCTGGGGCGGAAATTGTCTATAAGTAGGCATTTATTTCATGATTGATAT2290.11163271374739808No Hit
GTGGGTGGGGAACTGAATTATAATAGTGACACAACCTATCAAAACCTCTTGG2280.11114523464806447No Hit
ATTTTCGGGATCTAAGGAAATTGTAGTTCCATCCCTTCTATTTTTAGTCTTT2280.11114523464806447No Hit
TAGGCTGGGATCACACCAAAACCTCAGGCTACAAAAGCAACAATAAATAAAT2280.11114523464806447No Hit
ATCATCGGGGTAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAAGTT2280.11114523464806447No Hit
CTGACAGGGAGAATATGAAATTCTAGTGGAGGAACACTGGAACAGCTAAGAG2270.11065775554873086No Hit
ACACTCGGGTAGAAAAAGATCCTGGAAGTTTATTTGATCTGACATGTTTTTG2270.11065775554873086No Hit
CAGCCTGGGAAACAGAGCGAAACCTTGTCTCAAAAAATCAAAAAAACGGGAA2270.11065775554873086No Hit
TGTTGAGGGTTGGATGGATGGATGGAGGGATGGAAGGAGGGAGGGAAGGATG2260.11017027644939723No Hit
AAGCAGGGGAAAGAGTATATCAGAATCTTTTAAACAGGTAGCTAATTTTTTA2250.10968279735006362No Hit
TATGATGGGGTACAATTAGTAGTAATTTGAAATGATGCCTATGGTTTCCTAA2250.10968279735006362No Hit
GTTAAAGGGTGGAATCGTCTTTTTCCTCCTTTACGTCAGCGACAAAACGTAA2250.10968279735006362No Hit
ATCCTTGGGATAGAAACAAGGCAAATATAAACACCAACTCTCTGAAAGGAAA2240.10919531825072999No Hit
AAATGTGGGTAGAGAAGTCACCTCAAGATTGGTGACAGATCTTCTCATGTGA2240.10919531825072999No Hit
AAATATGGGGGTAACCAGCAGGCAGCTGAGAGCAAGAAGGCACGTGCTCTGC2240.10919531825072999No Hit
TGTGCTGGGAATTTATGCATGTAAAAAAATACAGGAGATGATTCACTGGAAT2230.10870783915139638No Hit
TTCCAGGGGAATTCTATGTACAGTTGACCTGTTTAGAAGTTGTATTGCCATT2230.10870783915139638No Hit
CCGCGAGGGTAAAATGCCAATTTTACTTTTTACTGTACTTTTTCTAGGAGAT2230.10870783915139638No Hit
GCAGCTGGGTTAAAAGCTTTTCCTGGAAGATACCCATTTAATGCAAAAGAGA2230.10870783915139638No Hit
TCATAAGGGTCGAGGGATGGCATTACCTCCGTGTTTCTCCCTTTCGAGCCGG2220.10822036005206277No Hit
ATGCTGGGATTATAGGCGTGAGCCACCACGTCTGGCACAAAAAAAATGTATT2220.10822036005206277No Hit
CTTAAGGGGATCCTGATGCTTAATATATGAAAGGTAGTTTTTAATTCAAAAG2220.10822036005206277No Hit
GGCGGTGGGAAATGTAAGACATAGTATTTCTGAGACTATATTTTCTCAAGTC2220.10822036005206277No Hit
TCATGCGGGCCAGCTTTCTTCTGTGTCTCAGAGGTCTCCTTACTCTGCTAGC2220.10822036005206277No Hit
ACAACAGGGATGATGAACCTTTTAAAAACCAGCTACTAATTTCTTGACATAA2210.10773288095272916No Hit
TCTCGTAGGGAATCTTACCTGCCAACATATTCACCATAATTTTTTTATTTTT2200.10724540185339554No Hit
ATATTGGGGCCTGTATTTTCTCCTGTTTCAGGGGGAAGGTGGCTCTGCCATA2200.10724540185339554No Hit
TGGCCCGGGGAAGTAACCCATCAAAATATGCTCTGCAGTGATTCCGCTTAAT2200.10724540185339554No Hit
TCGTCAGGGGGACTTGATGTTTGCGGTTTGAGAGGCAAGAAAATAAAATAAC2200.10724540185339554No Hit
TATACAGGATGCTAAACGCAGCGTATACATAACATGAACAAGACATAAAGCA2190.10675792275406193No Hit
ACAATGGGATGTTATGATACTTTAGTTCTTAGAAATGACAGGTTAAAAAATG2190.10675792275406193No Hit
CAGCTGGGGAATGAATGAATTAATCAATGAATGGAGAGACAAGGAGAATCAG2190.10675792275406193No Hit
CGGCCGGGATGAGACACCATTGCAAGAAAATTTTATCCCTCCAGAAGTATTT2180.10627044365472829No Hit
ACATCCGGGAATATTTGTATTGTTGGTGTAAATCAGCACATAAACTTCTGAA2170.10578296455539468No Hit
TTAGCAGGGGAACGATACGCCTACTGTGACCATTAAAGTTGTAACTAATTAA2170.10578296455539468No Hit
TAAACAGGGGCATAAAAAGAATCATGTTCCCTGTGTACATTTAAGAAAAAAA2170.10578296455539468No Hit
TGAGGTGGGATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA2170.10578296455539468No Hit
GACTATGGGATAACCGTAGTGGATCTACCAATACACCCAATGAGTGGCATAC2160.10529548545606107No Hit
CGCATAGGGATTGTAGTCCACAGGGAATGGATGCTTCACTTGATCTCTGGAC2160.10529548545606107No Hit
GTGAGAGGGGGTGGGCGGAAGAGGTGGCCCCGGATGCTGCGGCGCCCGCTGG2160.10529548545606107No Hit
GCTACAGGGGTCCTCTTCCTTCACTTCTTGTTGTAATTGCAGCCATTTTCAT2160.10529548545606107No Hit
CACACAGGGCAAGAAATTCTGTCAGTGTCTGAGTATTTATTTGAGAATGGTC2150.10480800635672746No Hit
TGGGCGGGGACAAAATTGTTAAATTACTAGTAATCCTTACCTAAGATTATTT2150.10480800635672746No Hit
TTAAGCGGGTAGAGAATATCAACAAAAATAAATTTTATAAAAGAACTAAGTG2150.10480800635672746No Hit
ATAACGGGTTAATAATCTAGTATTTCTTGGAGGAGCTAATTTGCAAGTTCTT2140.10432052725739384No Hit
CTGTTCGGGATCTCAATACAGAAGAGAAGGCAAAAATTGCATTTTAAAAACT2140.10432052725739384No Hit
ACATCAGGGACACTTGTTTTTCATTGCTTTAATTGATCAAATGATAGGTAAA2140.10432052725739384No Hit
AGCGAGGGAAGGTGGAAGGCTGTCTCTGCCACCTACCAGCTCTGTGATCTTG2140.10432052725739384No Hit
TTTTTTGGGGATCTTGCTCTGGGGGATCAGATACAGCTGGCACTATTGGTTT2140.10432052725739384No Hit
GACTGAGGGACTGTTGTACACTCATGATTGCAGTTTTTAGCTTAAGAAACTT2140.10432052725739384No Hit
CGTACGGGGGGCCTTGTAGGACTGACCCCTGTGCTTGCTGTCACGTTTAAAG2140.10432052725739384No Hit
AGCCTTGGGACAATTTATTCAGTCCATAATCACAGGATTTCTAATGCATTTT2140.10432052725739384No Hit
CAAAAGGGGAGGCCTCTTTAACAGGGAAGAGACAAATGACAATATTCAAATT2130.10383304815806023No Hit
GGCCATGGGCTGTGCCGTTGTACCTTTCCACAGCAAAGTGTAAGGAATCCAG2130.10383304815806023No Hit
CACTCGGGACCGAGGAGATGGAGCTGTACGAGCTGGCGCAGGCGGCGGGCGG2130.10383304815806023No Hit
GGAATTGGGCTGAGAACAGCCCATTTAAAAATTTCTAAATTGTGAAAAAATC2130.10383304815806023No Hit
TCATGCGGGATGTACATATATTGTCTGTCCATATGTATTTGTAAATAGGTTG2120.10334556905872662No Hit
CTGCTGAAGGGTTACAGGGTCTGTTTCACCCAGCCCGGAAAGTCAGAGATGT2120.10334556905872662No Hit
GTAGTTGGGGTGAATGGTATATTATTGGGTTTTAGTTTGCATTTCCCGAATT2120.10334556905872662No Hit
AGTCTAGGGAGCATCAGCAGGATGCCCTTTGGACTGAAAAACTCCAAGTCTT2110.10285808995939298No Hit
ACAACTGGAAATGTGCTGGGTCACACCTGAAGTCAGCAGGGCTCTGGGCCTC2100.10237061086005937No Hit
TGCATAGGGGGTCAATTGATGTGATTATTTTTGTTAAATGTTGTCTTGTGCC2100.10237061086005937No Hit
AAGCCTGGGTGACAGAATGAGACTCTGTCTCAAAACAACAACAAAAAGATTT2100.10237061086005937No Hit
GCTCCTGGGAGGTCTTTTAGGTTTAGATTTCATACATCTCTTCATGTTAGCA2100.10237061086005937No Hit
TAATCCAGGGTAGGCTGCCTTAGTAACAAAATAAAACAGATGTGTCATAATA2090.10188313176072575No Hit
ACCTATGGGGATCTTAGGAGGGGACATTAAAATTGGCAAATGCTACAAATCA2090.10188313176072575No Hit
TGTGCTGGGATATCAGTATGTTTAAAAGTGTCAGTACTTATGAAATTATAAA2090.10188313176072575No Hit
GACAAAGGGAGAAAAGAGCAAAATGTGAAAAGACACAAGACTCTCAGGTATA2080.10139565266139214No Hit
GAAAAGGGGCTCCAGCCTAGGTGACAGTGAGACTGTATCTCAAATAAATAAA2070.10090817356205853No Hit
TTGCAGGGTATAAAATTTTAGCCCACATTTCAGGCTCCGTAAATCATTTGAG2070.10090817356205853No Hit
GCAACTGGGTGCCTTGGGTTATTCCTGAAAGCTTCCTAATTGGTACCTGCTG2070.10090817356205853No Hit
TGTTTGGGGTGTCTTCAAAGGTGTGAGAGCTGAGGAATATGTGTTCCTATGG2070.10090817356205853No Hit
CGACTGGGTGGGCAAAACACAATTATTTTGGGGCTCTGAGATAGAGAAGTGA2060.10042069446272492No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)