FastQCFastQC Report
Sat 31 Dec 2022
SRR5847275.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847275.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203197
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT5950.29281928374926797RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC5920.29134288399927166No Hit
CTAATGCGGGGAGCATGGTATGTGTTTCCATTTGTTTGTGTCATCTGTGATT3180.15649837349960874No Hit
TGGGCAGGGTACCCTTTAATAGAAATTTTATCTTTTGTTATTTTGGTTAATT3140.15452984049961369No Hit
TTGCACGGGTAACATTTCCTGTCACTGTGTGTGGGAAAAATAGATATAACCA2930.1441950422496395No Hit
CTCCACAAGGGAAATGTGCTACTTTTTGATACTTACATTTTCATATGCTTTA2900.14271864249964322No Hit
TTCACAGGGATATGAGGTAACTCACCATCACAAAAATGACCAAAAAACGTTT2850.14025797624964936No Hit
AAAACGGGGTATGAAAAAATGTTCAGTCTCACTAGTAGTCAGGAAAATACAA2820.13878157649965306No Hit
GATGCTGGGATCTTTTGTAATGTCATTCATAGAAAACTGGTAAATGTGTTTC2810.13828944324965425No Hit
CAGACGGGGATCTGAAACTGTGCTGTGAGTTACTTTGCAGTCTAAAAGCAAA2800.1377973099996555No Hit
AATACCGGGGGGCAGAGAGAAGTGGTCAAAATCTGGATATATTTTGGAGGTT2790.13730517674965673No Hit
CACATCGGGACTGCGTATGTGTCTAATATTTGTAAAATAATTAGTTCAAAAC2770.1363209102496592No Hit
GATTTAGGGGAACTAATAAAATTTTATTTCTTAAATATAAATTTGACCTAAA2710.1333681107496666No Hit
GATAGAGGGATTGTTGAAGACGTGACTTCATAGCAATATATAGAGAATAAAT2710.1333681107496666No Hit
TGAGGTGGGATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA2700.1328759774996678No Hit
AAGCTAGGGTAACCCAAAGAGGGAGTCCTGGGAACCCCAATTTGAAGACAGT2680.13189171099967026No Hit
GGCCATGGGCTGTGCCGTTGTACCTTTCCACAGCAAAGTGTAAGGAATCCAG2660.13090744449967273No Hit
ATGTTGGGGCAGAGAGGAATGAGAAGAAGCAGCAGATGGGCAAAGAGTACCG2650.13041531124967395No Hit
AAGCAGGGGAAAGAGTATATCAGAATCTTTTAAACAGGTAGCTAATTTTTTA2640.1299231779996752No Hit
TCATGAGGGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC2620.12893891149967765No Hit
ACACTCGGGATGACTCCACATGAACCTTCACATTTGTTCGCTCATAATCTAC2610.12844677824967887No Hit
AAAATAGGGGTCTTAGAGGAAAAAATGTAAAGGTAAGTAATTAAGCATATGA2600.12795464499968012No Hit
TTACACAGGGGATTTCACTTATCACATAGTATTTCACATAATATCTATTGTT2590.12746251174968135No Hit
GCTCACGGGCACATCCACACTGATCTTACCCCATAATGAACTTGGCTCTGAA2590.12746251174968135No Hit
CACTGGTGTTTATTACAGAGTTTGACATACATGGGTTCATTTGCCATTTATT2590.12746251174968135No Hit
GCTTTGGGACTGAAGACAAACTGATTCTGGGCTGTGAAGATTCTTCGCTAAT2570.12647824524968382No Hit
ATCATCGGGGTAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAAGTT2560.125986111999685No Hit
AGCTTGGGGACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT2530.12450971224968874No Hit
TTTATGGGGAACAGCCTTGGTGTGACTTTGTTCCAGTAAACTTTCTTTATAG2520.12401757899968996No Hit
TCGTCAGGGGGACTTGATGTTTGCGGTTTGAGAGGCAAGAAAATAAAATAAC2520.12401757899968996No Hit
AAATATGGGGGTAACCAGCAGGCAGCTGAGAGCAAGAAGGCACGTGCTCTGC2510.1235254457496912No Hit
TAGGCTGGGATCACACCAAAACCTCAGGCTACAAAAGCAACAATAAATAAAT2490.12254117924969364No Hit
GGTCAGGGAAATGTTTCTTTCCATTTTGCTTTGTTTTTCTATATCGATCTAG2480.12204904599969488No Hit
ACATCAGGGACACTTGTTTTTCATTGCTTTAATTGATCAAATGATAGGTAAA2480.12204904599969488No Hit
TTGCCACGGGATCTCAGATAATATATTCTAATGATGAGAATAACTACTAAAA2470.1215569127496961No Hit
CCGGCTGGGAGTTCCTGACTTTTAAAATGGATGAAATTTTCTCTTTGTCTGA2460.12106477949969735No Hit
CTTTACGGGCTAGAAGAGAAAATTCCTAATATCCTGTCTGCATTTCCTTTCC2460.12106477949969735No Hit
TGGTTTGGGATTCTAACTAACTCCTTTTCTCTTTAAAAAAGCAAACTTCATT2440.12008051299969981No Hit
AATTTAGGGCGATCTAAATGGAGGGCAGTCAAGGTGAAAATTAAGCTCAATA2440.12008051299969981No Hit
ATAAGGGTAAACGCAAAACTTTTGTACTTTATTACGAGTAAAGTGTAATGAG2430.11958837974970103No Hit
ATCCCTGGGGCTGTGAAAGATGCCGCTTCCTTGTCACTTCAGAGAATGATGC2430.11958837974970103No Hit
TATGCCGGGAATCTAGTAATGGGACCAAGATAAGTAGAATTGTGTGAGAGGT2430.11958837974970103No Hit
CTGCTGAAGGGTTACAGGGTCTGTTTCACCCAGCCCGGAAAGTCAGAGATGT2420.11909624649970225No Hit
TTTTTTGGGGATCTTGCTCTGGGGGATCAGATACAGCTGGCACTATTGGTTT2420.11909624649970225No Hit
CCGCGAGGGTAAAATGCCAATTTTACTTTTTACTGTACTTTTTCTAGGAGAT2420.11909624649970225No Hit
CTGTCGGGTGTTGCAACATTTTTTAAAACACTGTAAAATACACCTGAAGTTT2420.11909624649970225No Hit
TTCAAGGGGGGATATCAGACTGGGAATTTTTATTTGGGACTACATCCCAGAC2410.11860411324970349No Hit
TTGCTTGGGCAGCTACATGATATGCTCATCTACATGCACGTTTGTATGTGAA2410.11860411324970349No Hit
TGCATAGGGGGTCAATTGATGTGATTATTTTTGTTAAATGTTGTCTTGTGCC2410.11860411324970349No Hit
TGCTCTGGGCGAAATGCAGCTTTTTCTGAACTTCAAAGATAGAATCCCATTT2400.11811197999970471No Hit
TATGATGGGGTACAATTAGTAGTAATTTGAAATGATGCCTATGGTTTCCTAA2380.11712771349970717No Hit
ACAATGGGATGTTATGATACTTTAGTTCTTAGAAATGACAGGTTAAAAAATG2380.11712771349970717No Hit
GACTTGGGGCAGGAAACATGATATAAACAAAATTTACATAAGCCTATAATCT2370.11663558024970842No Hit
TTATTGGGGAAGCCTTCTCTGAAGTATTGAGGGAAAGTCTGCTAAATGGCTA2360.11614344699970965No Hit
TCAACCGGGCTCTCAGCCCTCCTAATGACCTCCGGCCTAGCCATGTGATTTC2360.11614344699970965No Hit
GTATGTGGGCGATCTGTGGACGGGCCTATCTCACGTGCAAAGACACACATAG2340.1151591804997121No Hit
TTCGAGGGGCTTAGGTAGGGGTGAAGTCTCAGGGTATGGCTGAATTATAGTG2340.1151591804997121No Hit
CTGCCAGGGGTGGCTTAAAATAAAGAAAATGCAGCAAAAAGAATGCTATAGA2340.1151591804997121No Hit
TCTCGTAGGGAATCTTACCTGCCAACATATTCACCATAATTTTTTTATTTTT2320.11417491399971456No Hit
TAGTTTGGGTAACAACAGAACATCAAAGTATGAGGCAAACACTGAAAGAAGT2320.11417491399971456No Hit
TTGCTAGGGACTAGGAATTGGAATCAAACAATGCCACCCCAAGCGTAATTTT2310.11368278074971579No Hit
AATACAGGGCGATCTTTGTGTGGGAGTTTATCAAAGGTTTATGTAGTTTTGT2300.11319064749971702No Hit
ACTGTTGGGCGCTCTTCCGATCTGCCCACGGGACGGGAATAGTTTTCAACGT2300.11319064749971702No Hit
TGACACCGGGATAATCACAATATTTTATACTCACCTTTATTGACTAAAAATA2290.11269851424971825No Hit
ACAACTGGAAATGTGCTGGGTCACACCTGAAGTCAGCAGGGCTCTGGGCCTC2280.1122063809997195No Hit
GCAGCTGGGTTAAAAGCTTTTCCTGGAAGATACCCATTTAATGCAAAAGAGA2270.11171424774972072No Hit
ATGTATGGGGAGGGCGAGAAGATCCGAAAGAAATACCCGGACCGGGTGCCGG2260.11122211449972194No Hit
GTAGTTGGGGTGAATGGTATATTATTGGGTTTTAGTTTGCATTTCCCGAATT2260.11122211449972194No Hit
GCTCCTGGGAGGTCTTTTAGGTTTAGATTTCATACATCTCTTCATGTTAGCA2260.11122211449972194No Hit
TCCCTAGGGCTCAAGGAGCTAAAGAAAAACATGAATGGATGACTAAAATAAA2250.11072998124972318No Hit
CTGACAGGGAGAATATGAAATTCTAGTGGAGGAACACTGGAACAGCTAAGAG2240.1102378479997244No Hit
GCTAACACCTGGCAACCGCTGATTTTTTTTACTGTCTCCATAGTTTTGCCTA2240.1102378479997244No Hit
TTCCTAGGGTCGAAGCTGCCGCTGCAGACCATCATGAGGCTGCTGCAGGTGC2240.1102378479997244No Hit
CGCCACGGGATCAGTGAAACAGGAATTTATAAGCAAAGGAAAATGGATTGCT2240.1102378479997244No Hit
TGTGCTGGGGCGGAAATTGTCTATAAGTAGGCATTTATTTCATGATTGATAT2240.1102378479997244No Hit
ATCAGCGGGCTGGAGGATAATGAAAAGTCCGTGTTTTGAAGTTTTCAGATTA2240.1102378479997244No Hit
GGTTTTGGGAGATAATCTTTGGAATGTATGTTTTAAATGCTACTCATGAGTG2230.10974571474972564No Hit
CACTCGGGACCGAGGAGATGGAGCTGTACGAGCTGGCGCAGGCGGCGGGCGG2220.10925358149972686No Hit
AACACGGGGGGTAAAAACTATTTTTATTTGCCTATGATATACTTGGTTTCTA2220.10925358149972686No Hit
TTTACTGGGAGAACTGGACTTCCTCCTCCGGGAAGCCTCCCTTGACCTTCCC2210.10876144824972811No Hit
ACGCGGGAGTACTGTATTAGGCATTAAAAGGATGAAGATGACTAAGGGAACA2200.10826931499972933No Hit
CGAATAGGGAGGCCTTATTTCATATGTTTCCTCAACTGTACAATGAACTATT2200.10826931499972933No Hit
CAGCCTGGGAAACAGAGCGAAACCTTGTCTCAAAAAATCAAAAAAACGGGAA2200.10826931499972933No Hit
CAAAAGGGGAGGCCTCTTTAACAGGGAAGAGACAAATGACAATATTCAAATT2190.10777718174973054No Hit
AAACACGGGACAGTTCTCTACAAAAAGAACCAATGAACCCAATAGGAACAAA2180.10728504849973179No Hit
TTTGTGGGGATGGTGAGTTGGTTTGGGAGAGGAATCTCCCAGATCCCCGTTC2170.10679291524973301No Hit
GACAAAGGGAGAAAAGAGCAAAATGTGAAAAGACACAAGACTCTCAGGTATA2170.10679291524973301No Hit
GGTACTGGGATTTGAATGATGAGACTACCTCCAAGCAAATTAAATCCATGCT2170.10679291524973301No Hit
TGATCTGGGATGAGAACTACCTAATGACAATTTTTAGAGGTGGTTATCTCAA2160.10630078199973425No Hit
CTGTTCGGGATCTCAATACAGAAGAGAAGGCAAAAATTGCATTTTAAAAACT2150.10580864874973547No Hit
TTGCAAGGGCTTGTTTTCTGTGTAGTTTACCTTAAGTCCACTCTTGGCATTT2150.10580864874973547No Hit
ACATCCGGGAATATTTGTATTGTTGGTGTAAATCAGCACATAAACTTCTGAA2150.10580864874973547No Hit
CAAAATGGGCTTGACACGGGGTGGAGTGTGTGTCTTTCCTCCCTGAAGCTGT2150.10580864874973547No Hit
ACTTCTGGGCACATCCAGTTATGATATGCTTGTAAGCAGGCATTAATTCTTA2150.10580864874973547No Hit
TGCGATGGGGTATGAGGATAAGTATCAGATTCATGTAGTGATTTTCTCTTGG2150.10580864874973547No Hit
AGACATGGGCTTGCTGCACATCAGGAGATCTAGAAAGGAGACTTTTCTTCCT2140.10531651549973671No Hit
ACCTATGGGGATCTTAGGAGGGGACATTAAAATTGGCAAATGCTACAAATCA2140.10531651549973671No Hit
TGTTGAGGGTTGGATGGATGGATGGAGGGATGGAAGGAGGGAGGGAAGGATG2140.10531651549973671No Hit
GGTGGCGGGGCATAGGCTTAATTCTTGGTTATTTAAATGAATTTTTGAACAT2130.10482438224973793No Hit
TTGAGGGAATCCATGCTGCGTGCCTGTCCTATAATTGCCCATATAGTCATCC2130.10482438224973793No Hit
GAAAAGGGGCTCCAGCCTAGGTGACAGTGAGACTGTATCTCAAATAAATAAA2120.10433224899973918No Hit
AGTTTGGGATTCTTTTTACCCTGAGTGAGCACTTTTCACTTTCCAGCTAGGT2120.10433224899973918No Hit
CTTTCGCGGGGAGTGTGCTATTATTTTCTGGTTAGAAAGCTTCGTATGATAA2120.10433224899973918No Hit
TCTGCGGGAAAAATCAGAAAGTATGAGATCCTGTTATGTTAAGGGAAATCCA2120.10433224899973918No Hit
CTTAAGGGGATCCTGATGCTTAATATATGAAAGGTAGTTTTTAATTCAAAAG2120.10433224899973918No Hit
AACTTAGGGTAAGTGATATCAGCCTGTCAGAAAGAACAGATACTGTGTGATT2120.10433224899973918No Hit
AAGCTAGGGGATTCTCAGGGTGATGTAGGGGAAAGCATAAAGAAGGCTGAGT2110.1038401157497404No Hit
TATTATGGGCAAAGCAGGTGGCATTTTACTGAACAATTAAATGTTTGAAGTA2110.1038401157497404No Hit
TTGTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2110.1038401157497404No Hit
TACGCCAGGGGCTCCTAATTGTTAGATGTTAGGGGCCACAATGCTAACTGTG2100.10334798249974161No Hit
GATCTGGGCCTTGGATTGGTAGTATAGTGAAATGATTAGGGCAGGGCTTTGA2100.10334798249974161No Hit
TCAAAGGGAAGTTCTAGATGTTATTTATAGTCTACAGTGAATTTTGGTCTGT2100.10334798249974161No Hit
CTATTAGGGAGTGGGGAAAAGTAGAAAGCTTTTCTCCTGGGATCGAGAACAA2090.10285584924974286No Hit
TGTGCTGGGAATTTATGCATGTAAAAAAATACAGGAGATGATTCACTGGAAT2090.10285584924974286No Hit
ACACTCGGGTAGAAAAAGATCCTGGAAGTTTATTTGATCTGACATGTTTTTG2090.10285584924974286No Hit
ACTATCGGGCAAGCTCTAAACTGATATATACACTGCAGAGACTAGGAGACAA2090.10285584924974286No Hit
AAGGCTGGGACCTAACCCTTAGAAAATGAATGTTAGAAGGTGCCTGCCGAGG2090.10285584924974286No Hit
CCCAGGGGGGGGATAGATAGATAAGATCAATAAGATAGGTAGATAGATGGAT2090.10285584924974286No Hit
TAAACAGGGGCATAAAAAGAATCATGTTCCCTGTGTACATTTAAGAAAAAAA2090.10285584924974286No Hit
CGTACGGGGGGCCTTGTAGGACTGACCCCTGTGCTTGCTGTCACGTTTAAAG2090.10285584924974286No Hit
CGACTGGGTGGGCAAAACACAATTATTTTGGGGCTCTGAGATAGAGAAGTGA2090.10285584924974286No Hit
GCTACAGGGGTCCTCTTCCTTCACTTCTTGTTGTAATTGCAGCCATTTTCAT2090.10285584924974286No Hit
TCATGCGGGATGTACATATATTGTCTGTCCATATGTATTTGTAAATAGGTTG2080.10236371599974409No Hit
TCTTTTGGGGTGCATAGGTGAGGATCACCAGCTAGTTTTGACCAAAAAGTCT2080.10236371599974409No Hit
TATACAGGATGCTAAACGCAGCGTATACATAACATGAACAAGACATAAAGCA2070.10187158274974532No Hit
ATATAAGGGAAGAAACTGAAGTTTAAAAATATTTGTGATCCAGAGTGATTCA2070.10187158274974532No Hit
TTGCAGGGTATAAAATTTTAGCCCACATTTCAGGCTCCGTAAATCATTTGAG2070.10187158274974532No Hit
TGGCCTGGGGGACCAGTACATCAGAGAGCAACGCGAGAAGGATTCTGTCATG2070.10187158274974532No Hit
AGTCTAGGGAGCATCAGCAGGATGCCCTTTGGACTGAAAAACTCCAAGTCTT2060.10137944949974655No Hit
GCTAGAGGGCATTCCTCATGGGTTATGAGTTGCTCCACAGGATTCTGGATGT2060.10137944949974655No Hit
TGATACGGGACTATTCATCCCCATCTTAAGTTTTACAAGGTGATGTAATCAG2050.1008873162497478No Hit
TGGCCCGGGGAAGTAACCCATCAAAATATGCTCTGCAGTGATTCCGCTTAAT2050.1008873162497478No Hit
GTTGCTGGGCTTGGAAATAACCCCTACATACGTACCTGAGTTTCTGATTGTT2050.1008873162497478No Hit
TGCCTGGGGCCCTATAGTTTTCAAAGCAAAAGAAAACTGAGTAATCTTGATT2040.100395182999749No Hit
TGTTTGGGGTGTCTTCAAAGGTGTGAGAGCTGAGGAATATGTGTTCCTATGG2040.100395182999749No Hit
CGCATAGGGATTGTAGTCCACAGGGAATGGATGCTTCACTTGATCTCTGGAC2040.100395182999749No Hit
AGTGCTGGGTAAAGACAGGAGTAAGAAATTATAAAAGTATTAATTTGGGGAA2040.100395182999749No Hit
AAATGTGGGTAGAGAAGTCACCTCAAGATTGGTGACAGATCTTCTCATGTGA2040.100395182999749No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)