FastQCFastQC Report
Sat 31 Dec 2022
SRR5847274.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847274.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284035
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT8880.31263752706532644RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC7930.2791909447779323No Hit
TCTTAAGGGGAGACGAGGCTGCGCCCGGATTCCGGTCCGCAGGGAGACCGAA3410.12005562694738325No Hit
TAGGCGTGGGTTCCCAGGCAGGGAGGGAAACCCGGGGAACTGGTTTTTCTGT3160.11125389476649004No Hit
ACTATGGGTATTCTAAAGCATAAAATTACTGTGTTTTGAGATCGTTTTCCCT3160.11125389476649004No Hit
AATATTGGGGTCCGAGGTTACTGTCCCCGGCGCGTCCTCTGTTGCCCCAGTC3130.11019768690478286No Hit
TTTCAAGGGAGTGAACCATTTTTATAGTATTCTTATGACAAAAAGTTGCCCT3130.11019768690478286No Hit
TGCAATGGTATGATTTTGCTCTGTGTACAAAGACAATGTTATCTCCAAAGTC3110.10949354833031141No Hit
CACTTCGGGTGGGCAGGGATAACTATTAATTTTCTTTTGAAAATTTCCCCTT3030.10667699403242557No Hit
ACGCATGGGGTATTTGTGTCTACCTATAATGGCTAAAAATTGTCCACTATGT3010.10597285545795411No Hit
GAAAATGGGTTGCAGAAATGACTACATTAGGTTATTTGGAAATAGCGGTGCT3000.10562078617071839No Hit
AAGTAAAGGGCTTTTGGCATTGAAAAGAGATAATGGGTGATTACACTGTCAA2930.1031563011600683No Hit
GTGGTTGGGATGGCAAAACCACATCTCTACTAAAAATATAAAAATTAGCCAG2860.1006918161494182No Hit
CATCTAGGGGACCTGGATTTAAATGGTCATGAGAGAAATTTCTGATATGATA2860.1006918161494182No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)