FastQCFastQC Report
Sat 31 Dec 2022
SRR5847273.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847273.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284071
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT8580.30203716676464687RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC8010.28197176058098156No Hit
ACTATGGGTATTCTAAAGCATAAAATTACTGTGTTTTGAGATCGTTTTCCCT3390.11933636309232551No Hit
TTTCAAGGGAGTGAACCATTTTTATAGTATTCTTATGACAAAAAGTTGCCCT3370.11863231375254778No Hit
ACGCATGGGGTATTTGTGTCTACCTATAATGGCTAAAAATTGTCCACTATGT3250.11440801771388139No Hit
TCTTAAGGGGAGACGAGGCTGCGCCCGGATTCCGGTCCGCAGGGAGACCGAA3150.11088777101499273No Hit
TGCAATGGTATGATTTTGCTCTGTGTACAAAGACAATGTTATCTCCAAAGTC3140.11053574634510385No Hit
TCTCTTGGGAACAACAGCCTTTTTGGTAGTCACATAGCAGAATGATCAGAGT3050.10736752431610407No Hit
TGTGATTGGGTCATGAATAGAAGACTCATTCTTACCATAGACATTAGCAACC3050.10736752431610407No Hit
AATATTGGGGTCCGAGGTTACTGTCCCCGGCGCGTCCTCTGTTGCCCCAGTC3030.10666347497632633No Hit
CATCTAGGGGACCTGGATTTAAATGGTCATGAGAGAAATTTCTGATATGATA2970.10455132695699315No Hit
TCTATGGGGGGGAAAAGGGAGTGCAGGCCGCGGTGGGGTGGGGCGGCGAAGG2950.10384727761721542No Hit
AATAGTGGGATGGCTTTTGGGTATCTTGTCTTGCTTAGAAAATATTTTTATA2900.1020871542677711No Hit
GCTGCCGGGTATTACTTTCAGTGTACTTGGCAATCTACTGCTTTCTGAGATT2890.10173512959788222No Hit
TGCTTGGGGATGGAAGGTTTGGCATGGCTGCGGCCCTGGCAGGTACCATGAG2880.10138310492799336No Hit
GAAAATGGGTTGCAGAAATGACTACATTAGGTTATTTGGAAATAGCGGTGCT2880.10138310492799336No Hit
CAAAGCGGGCACAGAAATTCTAGTATTCTGCACTTTTATAACTGCTTTCATC2870.10103108025810449No Hit
GCAGCGGGGATCCTCCACTGCTCCCCGCGGGCACTAACATTGCCGATGAGAC2850.10032703091832676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)