FastQCFastQC Report
Sat 31 Dec 2022
SRR5847272.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847272.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281082
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT7820.27821062892679005RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC7330.2607779936104055No Hit
TGCAATGGTATGATTTTGCTCTGTGTACAAAGACAATGTTATCTCCAAAGTC3350.11918230267324126No Hit
TTTCAAGGGAGTGAACCATTTTTATAGTATTCTTATGACAAAAAGTTGCCCT3280.11669192619947204No Hit
ACTATGGGTATTCTAAAGCATAAAATTACTGTGTTTTGAGATCGTTTTCCCT3210.11420154972570282No Hit
CTCTCACGGGGCATTCAGCTAATTTCAGAGATGTCCTTTATTTGAGGTAGAT2990.10637465223671384No Hit
AGACAAGGGACTGAGGAGATCATCTCAAAGGTGATGCCAAACGAGTCTGGGG2940.10459581189830726No Hit
TCTCTTGGGAACAACAGCCTTTTTGGTAGTCACATAGCAGAATGATCAGAGT2910.1035285076952633No Hit
AATATTGGGGTCCGAGGTTACTGTCCCCGGCGCGTCCTCTGTTGCCCCAGTC2900.103172739627582No Hit
TGTAGTGGGGTACTGAATTGGACATAAAAATATTGACATTCTAAGGAGAGAT2880.10246120349221935No Hit
CAGCTAGGGGGTCAGTAGCAAAAATCAATCGTGTCTATACATTGGCTTCAAG2880.10246120349221935No Hit
ACAAGCGGGAGAACACAATAGCAAACGTGAGAAGAAGATTGTAAGTTCCGGA2870.10210543542453804No Hit
TCGTTGGGGAAATATAGTAAATCTGTTCACCTATCTGCTATGCTAGCAAATT2850.10139389928917542No Hit
ACGCATGGGGTATTTGTGTCTACCTATAATGGCTAAAAATTGTCCACTATGT2820.10032659508613145No Hit
GCTGCTGGGTCTGTATCTTTTAAAATACACCTACCTCATACTTTGTGTGAAG2820.10032659508613145No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)