FastQCFastQC Report
Sat 31 Dec 2022
SRR5847271.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5847271.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221587
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGC6250.28205625781295834No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTTGTGGATCTCGTATGCCGTCT6130.27664077766294953RNA PCR Primer, Index 23 (95% over 21bp)
CGAATAGGGCTATGGAGATCTGGATAAATTACATTTTATATTTGATTTTGAT2810.12681249351270607No Hit
CTTCATGCGGGCTTGAGTTCTCGGCAATAATGAGTGATTATTGTTATTTTTA2780.12545862347520387No Hit
ACACAAGGGCCTCTGCCTCCCGGGTTCAAGTGATTCTCCTGCCTCAGCCTCC2730.1232021734127002No Hit
AAGTTAGGGAGGAAAGGTTGGCCAAAGTTTTTATGTTTCTTTTTTATTTTCT2720.12275088340019946No Hit
TTTTGTGGGACTTCACCTCTGAGCCTCCGATGCTCTGTGAAATGGAAGGAAT2650.11959185331269434No Hit
CAATGCGGGATGGGGGTTCCTTAAGATTTCAAAACTGCTGTATGATCTGGCT2640.1191405633001936No Hit
CCGCTAGGGATGCCCGGCTAATTTATTTTTAGTAGAGATGAGGTTTCACCTT2590.11688411323768992No Hit
TTCACTTGGGATTCCTCACAGTTCTGGATGCTGGGAAGCCCAAGATCAGAGT2550.11507895318768699No Hit
ATTAAAGGGTGCATTTTTGTACCTGAGTTGGAGTGACATGGCACAGATGGCA2510.11327379313768407No Hit
CACAACGGGAATGTATCGCAAATTCACAGGTTTCAATATGAGGAGATAATTT2500.11282250312518334No Hit
GACAGGGGCAAAATTTAAACTTAGAGTTGTGCTTCTAGAAATGGAAGCTTTT2470.11146863308768114No Hit
TGCTCTGGGAGTCAGTCTAGCTAAACGTTTGTCAATTTTGTTTCTCTTTTCA2470.11146863308768114No Hit
TCTAGTGGGTTCAAATGAGAGAATAAGACCACTTGGTGTCTAAAGTCTTTCT2470.11146863308768114No Hit
GCTGCAGGGATGGCAGGATGGGGGGTACTGTGTAGATGATTAACTGACTTTT2460.11101734307518041No Hit
ACGAATGGGGTAGTTGCAGCTGATGCCTGAATGTATCCTAGCTGACAAATTA2450.11056605306267966No Hit
TTCGTAGGGGCTTGGAGAATAAATGAGTGAATGAATGCATGGATAAAATTGG2440.11011476305017895No Hit
GATTAAGGGGTCAGGGCTTGCTTTGACCCTGCTTCGTTCCACCCCGGGTCCT2440.11011476305017895No Hit
TTGCTTGGGGCCCCAGTCACTGAGCCGCCGCCGAGGATTCAGCAGCCTCCCC2430.1096634730376782No Hit
CACGTAGGGAGGAACTGTGAAGTTAACTGTCTCATGGGAGATTTCTGTTAAA2410.10876089301267673No Hit
GGTGGTGGGTCTTGCGAAGGGATTGTAGTTTTGATTTGCATTTCCCAGATCA2400.10830960300017599No Hit
AGCAATGGGGAAAGAAATGAAATGGGAAGCAAACAAACAGAAGATCAAAAAA2400.10830960300017599No Hit
CACAAAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2390.10785831298767527No Hit
TGGGAAGGGAGGAAAAGCTGGTTTCTATTATATGATTTTCCTACATATGGCA2390.10785831298767527No Hit
ATTCTTGGGGAATGAGGGAGAGAGGGAACGGGAGAAGGAGGGATGGAAGTAG2390.10785831298767527No Hit
AATCAGGGTCGCTTTGTGTGGTCTGATAAATACATTGATATTTAGGTTGAAA2380.10740702297517453No Hit
TCGTCAGGGATCCAGACTTTATTTTTCCACTTAGACAGCACAGGCAGAGTGC2380.10740702297517453No Hit
GGAACGGGGGACTTAAGAATGTATTTTCAGCCCAAGGAACAATTTTCCTGTT2380.10740702297517453No Hit
TCCAACGGGACTGGTCTGCTTGAGTGAATACACTGGAAAAAGAGGGGAAATG2380.10740702297517453No Hit
CACAGTGGGATAGGCATATTTTCAGCCAATTCAGCTTAATTCAAACATAACT2370.10695573296267381No Hit
AAGACTTGGGAGCAACGTGAAAACAGACAAATGCAAGTATCAAATAAATGTC2370.10695573296267381No Hit
GCGTTAGGGACATTAAAATAAGTACATGTGTTGATACACACATACCACATAA2350.10605315293767234No Hit
AAGAATGGGATTGGTAAGATGTGAAGTCCCAATGAAAGGCAGCTTCTGATTT2350.10605315293767234No Hit
TGCTATGGGGAGCCTGACCAACATGGAGAAACCCCGTCTCTACTAAAAATAC2330.10515057291267088No Hit
AGAAAAGGGATTGAGGAAATTGGGTGTTGGATGCAAAGCTACAGCACGTCTT2330.10515057291267088No Hit
ACAACTGGGAACCCTGATGCTGCTAGCCGGGCTATTGATTATGTACAGAGAC2330.10515057291267088No Hit
AGTGAGGGGAGAAGACACTTCCCTGAGCCAGCAGAAGGGACAGGTGCAGCAG2300.10379670287516866No Hit
AAGCATGGGAGATCTGACTTGTGGCAAACTTAGTGTTTGCTGTATGCCAAAT2280.1028941228501672No Hit
GGGATGCTAGGGTAATTTAAAAACACAAAAAAAATTTTAGCTCTTCCCACTT2280.1028941228501672No Hit
CCTGTAGGGATAAATGAATGAGCATGGCTATGTTCCAATAAAACTTAATTGA2280.1028941228501672No Hit
TAAGGGGAATAGAAGTGGTAAGAGTGGGCATTTTTGTCTTGTTTTGGGTGTC2280.1028941228501672No Hit
AAATAAGGGAACAACTTTTATCTATCAATTTCCATTACAGAATCAAGAATAT2270.10244283283766646No Hit
TCATGCGGGATGCATATATGTAGTGAAGTAATTACTACAGTCAAGTAAATTA2260.10199154282516573No Hit
AGCTACGGGGATTTCAGACATCTGGCCTCCAGGATTGTGAGGGAATAAATTT2250.10154025281266499No Hit
TAGTCCGGGTCTCTACACGGGACTCTAAAGGAATCAACGTTAATTCAAAAAA2240.10108896280016427No Hit
AAGGTTGGGACTCTTGGTGGGTTCCAGTAATCCATAGCTTAACAAGCCCTGC2240.10108896280016427No Hit
ATAACTGGGTGAAAGAGTTGTCCATGCTCTTTATTCATGCCCTCACTGAATC2240.10108896280016427No Hit
CATTGTGGGGTACAAAGCATTGTAATTTTATGTATCTCTACAGAGGAATATG2230.10063767278766353No Hit
TCAAACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2220.10018638277516281No Hit
TATAATGGGTGACAGCACATTGCCAGGAAGCAGGCCTGTAGTGTAAATTGTT2220.10018638277516281No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)